Pathogenetic Mechanisms of Formation of External Genital Endometriosis and Its Relapses in Patients of Reproductive Age

Purpose: To reveal peculiarities of angiogenesis from the pint of autoparacrine cellular regulation and to amplify the mechanisms of formation of external genital endometriosis.Materials and Methods: 352 patients were divided to 3 clinical groups: I - 148 patients with I-II stages of EHE, II group -184 patients with III-IV stage, 24 ones among them were with relapse. The III control group was presented by 20 patients without endometriosis. We used clinical methods, clinicolaboratory methods, genetic, morphological, biochemical.Results: On the systemic level the disorders of auto–para– and intracrine cellular regulation of angiogenesis with high production of EGF, bFGF, IGF-1, VEGF, solutable receptor of EGF and sFlt and low production of leptin. On the local level we found severe decrease of apoptosis, which was devoted to IL-8, NO, FSH and the simultaneous reinforcement by IL-8 of angiogenesis activity in endometrioid heterotopies and also the capillary vasodilatation with participation of NO. The increase of VEGF production on systemic level and high content of solutable receptor of VEGF, NO and NO- syntaze on the local level (the peritoneal fluid) are typical for relapsing of EGE.Summary: The switсhing of disorder of regulatory control of the cellular metabolism from one growth factors to another is happened in different stages of external genital endometriosis formation

Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products

A collaborative interlaboratory study on the method of direct quantitation of volatile compounds in spirit drinks and alcoholic products was conducted. The discussed method applies ethanol, the major volatile component of an alcoholic product, as an internal standard. In this study 9 laboratories from 4 different countries were supplied with standard solutions for gas chromatographic measurements. Five aqueous ethanol 40% (v/v) standard solutions containing target compounds in concentrations ranging from 10 mg/L to 400 mg/L of absolute alcohol were prepared and sent to the participants for quantification of acetaldehyde, methyl acetate, ethyl acetate, methanol, 2-propanol, 1-propanol, 2-methyl-1-propanol, 1-butanol and 3-methyl-1-butanol. The interlaboratory study was evaluated according to the ISO 5725 standards and the Eurachem guide. The within-laboratory precision varied between 0.4% and 7.5% for all samples and compounds, showing a sufficiently high repeatability of the method. The between-laboratory precision was found to vary within a satisfactory range of 0.5% ÷ 10.0%. Precision of the method was well within the range predicted by the Horwitz equation for all analytes. The analysis of trueness showed that the bias of the method is insignificant at the significance level α = 5%. The determined concentrations of the analytes compared well to the gravimetrical values thus showing very satisfactory accuracy of the method. The results of the interlaboratory study confirmed that “Ethanol as Internal Standard” method is robust and reliable and can be used as a standard reference method for analysing volatile compounds in water-ethanol samples. The possibilities of method validation according to the previously obtained experimental data were shown