MYOPATHY AS A DESTABILIZING FACTOR OF MEAT QUALITY FORMATION

This review paper is devoted to myopathy of slaughter animals and poultry, and examines a relationship between fast growth of muscle tissue in hybrid pigs, broiler chickens and turkey, and high frequency of detection of spontaneous or idiopathic myopathies. The development of myopathy reduces consumer and technological properties of meat, and leads to emergence of different pathological conditions (PSE or RSE meat, «destructured meat», «white» or «green» meat, punctate hemorrhage, «wooden breast» and others). Two types of myopathic conditions are examined: myopathies caused by stress in animals and nutritional myopathies, which contribution to meat quality deterioration seems to be determinative. It is shown that the basis of the mechanism of the myopathy development is the mechanism of the successive changes in muscle tissue: damage of cell membranes and release of mitochondrial calcium, which causes hypercontraction, dystrophic changes, atrophy and necrosis of muscle fibers. To alleviate the damaging effect of two types of myopathies, different substances-adaptogens (selenium, vitamin E, flavonoids and others) can be used. It is stated that the requirements of animals in adaptogens change with an increase in the indicators of their productivity.This review paper is devoted to myopathy of slaughter animals and poultry, and examines a relationship between fast growth of muscle tissue in hybrid pigs, broiler chickens and turkey, and high frequency of detection of spontaneous or idiopathic myopathies. The development of myopathy reduces consumer and technological properties of meat, and leads to emergence of different pathological conditions (PSE or RSE meat, «destructured meat», «white» or «green» meat, punctate hemorrhage, «wooden breast» and others). Two types of myopathic conditions are examined: myopathies caused by stress in animals and nutritional myopathies, which contribution to meat quality deterioration seems to be determinative. It is shown that the basis of the mechanism of the myopathy development is the mechanism of the successive changes in muscle tissue: damage of cell membranes and release of mitochondrial calcium, which causes hypercontraction, dystrophic changes, atrophy and necrosis of muscle fibers. To alleviate the damaging effect of two types of myopathies, different substances-adaptogens (selenium, vitamin E, flavonoids and others) can be used. It is stated that the requirements of animals in adaptogens change with an increase in the indicators of their productivity

Comparison of the blood parameters with the chemical composition of the muscle tissue of meat-and-egg chicken

Basic blood and muscle tissue parameters have been analyzed in crossbred male Russian White and Cornish hens (♂, RW x CORN, n=95, slaughtered at 63 days of age). According to BW at slaughter, males (n=95) were divided into 3 groups (group 1–1,000–1,799 g, n=31; group 2–1,800–2,099 g, n=28; group 3–2,100–2,650 g, n=36). It has been found that with an increase in the live weight at slaughter, the ratio of albumin to globulin (p=0.038), aspartate aminotransferase (p=0.003) increased in the serum of birds; the levels of globulins (p=0.05), glucose (p=0.02), Ca (p=0.006), Mg (p=0.05) decreased. With increasing BW, the crude protein content in thigh muscle decreased (p=0.019) against a trend towards increasing moisture content in thigh meat (p=0.058). Comparative assessment of biochemical blood parameters of nitrogen, carbohydrate-lipid, mineral metabolism, antioxidant protection parameters, some clinical blood parameters (hematocrit, erythrocytes and hemoglobin) and chemical composition of the breast and thigh muscle tissue has been carried out. The analysis (Pearson correlation coefficients) has revealed patterns between the concentration of some blood metabolites and the composition of muscle tissue in males. Thus, the accumulation and analysis of data on resource genetic populations is of interest for science and practice in order to establish relationships between blood parameters and the quality of chicken products, as well as to identify biomarkers for predicting poultry productivity in vivo

Histological characteristics and functional properties of red and white parts of m. semitendinosus of slaughter pigs

A unique muscle of pigs (Sus scrofa domesticus) is m. semitendinosus, which contains the “red” (dark) part located mainly in the depth of the leg cut and the “white” (light) part located in the close proximity to the subcutaneous fat layer. Differences in the characteristics of its “red” and “white” parts can exert a significant effect on quality and economic indicators of meat products. The aim of this research was to study histological features of the microstructure and technological properties of muscle tissue from different parts of m. semitendinosus, obtained from slaughter pigs of Russian production. M. semitendinosus was excised from chilled porcine carcasses (N=20) 24 hours after slaughter in the process of deboning. Histological examination showed that the dark part of the muscle was characterized by a higher package density of fibers, higher number of capillaries and higher sarcomere length. On the contrary, the light part was characterized by a higher diameter of muscle fibers. Analysis of muscle fiber types showed that the proportion of type I, intermediate and type IIb fibers was higher by 9.3, 5.2 and 4.1%, respectively, in the dark part. Significant differences between the dark and light parts of m. semitendinosus were revealed in terms of the number and size of giant fibers: the light part was characterized by a larger number (by more than 5 times) of giant fibers with the fibers of a larger size (almost by 11%). The samples of minced meat from the dark and light parts showed significant (р<0.05) differences in the mean values of lightness, redness and yellowness (L*, a* and b*) by 6.00, 4.68 and 3.01 units, respectively, in raw samples, and by 6.53, 2.99 and 1.81, respectively, after curing with the nitrite mixture and cooking (р<0.05). The dark part of m. semitendinosus had higher pH values (р<0.05) both for raw and cooked samples. The consistency of the samples from the light part was less elastic, looser and more crumbly than that in the samples produced from the dark part of m. semitendinosus, which was confirmed by the structural-mechanical investigations. Therefore, this study showed significant differences between the dark and light parts of m. semitendinosus by microstructural and functional-technological characteristics. Significant variability by muscle fiber diameter, which was observed in the light part of this muscle, apparently should be taken into account in breeding work and quality assessment of pork from slaughter animals

Postslaughter state of muscle tissue of pigs depending on the duration of pre-slaughter fasting

Changes in the muscle tissue microstructure lead to changes in meat quality. One of the causes of the myopathy development is animal stress. Pigs experience the strongest stress during pre-slaughter holding. The study of the postmortem meat structure depending on fasting time is a topical task. The objects of the research were samples of m. L. dorsi obtained after slaughter from pigs that differed in fasting time: 4 (group 1), 8 (group 2), 10 (group 3), 16 (group 4) and 18 (group 5) hours (N = 20, n = 4). Investigation of the microstructure and morphometric measurements were carried out on preparations stained with hematoxylin and eosin. Myopathic changes in muscle tissue were assessed using a semi-quantitative method developed earlier. All studied samples were characterized by the uniform condition of muscle tissue. Statistically significant differences between individual groups were observed regarding the number and area of giant fibers, sarcomere length, diameter of muscle fibers and proportion of muscle fibers, which diameter was lower or higher by 1/3 than the mean fiber diameter. An increase in the pre-slaughter holding time reduced the number and area of giant fibers (r = –0.8437 and –0.5796, respectively), as well as the diameter of “normal” fibers (r = –0.5337), which positively influenced pork quality. Groups 1, 2 and 3 were characterized by the presence of signs of moderate and pronounced myopathy. Only one carcass with pronounced myopathic signs was revealed in each of groups 4 and 5. In group 4, one carcass did not have signs of myopathy. Pre-slaughter holding during 4, 8 and 10 hours led to deterioration of pork quality. The recommended fasting time is 16 hours

EXPRESSION OF TLR2, TLR3, TLR4 AND PROINFLAMMATORY TNF AND IL-6 CYTOKINES IN LIVER BIOPSIES OF NONALCOHOLIC FATTY LIVER DISEASE PATIENTS

Non-alcoholic fatty liver disease (NAFLD) is a group of conditions closely associated with obesity that are among the most common and socially significant liver diseases in the modern Western world. The emergence and progression of NAFLD from simple steatosis to non-alcoholic steatohepatitis with the subsequent development of fibrosis are the leading factors in the pathogenesis of a significant proportion of the most severe liver pathologies, such as cirrhosis and hepatocellular carcinoma, as well as extrahepatic metabolic complications of NAFLD, such as insulin resistance and type 2 diabetes mellitus. The inflammatory component is one of the most important factors in the pathogenesis of NAFLD, particularly in the context of the progression of simple steatosis to non-alcoholic steatohepatitis. At the same time, the role of the most important mediators of the inflammatory response, innate immunity receptors and the Toll-like receptors in particular, in the pathogenesis of NAFLD has been poorly studied. In the present work, we first used the bioinformatics analysis of the publicly available gene expression databases to demonstrate that only TLR1, TLR2, TLR3 and TLR4 were significantly expressed in the healthy human liver. We then used the reverse transcription PCR to measure the mRNA expression levels of TLR2, TLR3, and TLR4, as well as those of the important pro-inflammatory mediators tumor necrosis factor (TNF) and interleukin-6 (IL-6), in the liver biopsy specimens obtained from 20 patients with NAFLD (simple steatosis, n = 10; non-alcoholic steatohepatitis, n = 10), as well as from 4 obese patients with clinical suspicion for NAFLD but no histological signs of NAFLD in their liver biopsies. We found a significant increase in the expression of TLR2, TLR3 and TLR4 mRNA in liver biopsy samples obtained from patients with non-alcoholic steatohepatitis as compared to those obtained from controls without histological signs of NAFLD. We were also able to demonstrate the association between the hepatic levels of TLR2, TLR3 and TLR4 mRNAs with the histological degree of liver damage as evidenced by the degree of steatosis and balloon dystrophy of hepatocytes, as well as with the plasma levels of uric acid, the important endogenous stimulator of innate immunity. Our data indicate the possible involvement of innate immunity, particularly the Toll-like receptors, in the pathogenesis of NAFLD

Роль кормовых адаптогенов при формировании концепции органического производства продукции свиноводства

One of the priorities of the Strategy of scientific and technological development of the Russian Federation (approved by Presidential Decree № 642 of December 1, 2016) is the transition to a highly productive and environmentally friendly agriculture. Organic agriculture can help to ensure sufficient food for the population by 2050 and simultaneously reduce the impact of agriculture on the environment only if it is implemented as part of a system of agricultural production, which should provide the same volume and composition of products as in the baseline scenario. An important aspect of organic livestock production is to reduce the impact of stress factors on animals. A promising solution can be the use of natural biologically active substances with pronounced antioxidant properties. The action of natural bioflavonoids (Taxifolin) and also in a complex with vitamins on the organism of fattening pigs during the periods of stress of various etiology (climatic, fodder, social etc.) has been studied. A complex including vitamin C, vitamin E and Taxifolin has been developed. The provisions of the method of feeding pigs as a way to reduce the negative impact of stress factors on meat quality by means of the studied nutritional factor have been studied experimentally.Одним из приоритетов Стратегии научно-технологического развития Российской Федерации (утверждена Указом Президента Российской Федерации от 1 декабря 2016 г. № 642) является переход к высокопродуктивному и экологически чистому агрохозяйству. Органическое сельское хозяйство может способствовать обеспечению достаточного количества продовольствия для населения к 2050 году и одновременному снижению воздействия сельского хозяйства на окружающую среду только в том случае, если оно будет реализовано в рамках системы сельскохозяйственного производства, которая должна обеспечивать те же объемы и состав продукции, что и при базовом сценарии. Важным аспектом получения органической животноводческой продукции является снижение воздействия факторов стресса на животных. Поэтому существует острая необходимость в разработке эффективных и устойчивых подходов к управлению для смягчения негативных последствий стрессов и повышения эффективности конверсии корма при получении свинины более высокого качества. Перспективным решением может быть применение природных биологически активных веществ с выраженными антиоксидантными свойствами, которые могут посредством стабилизации свободнорадикального окисления повышать адаптационные свойства организма к воздействию стресс-факторов среды. Изучено действие природных биофлавоноидов (таксифолин), а также в комплексе с витаминами на организм откармливаемых свиней в периоды стрессов различной этиологии (климатического, кормового, транспортного, социального и т. д.). Разработан комплекс, включающий витамин С, витамин Е, таксифолин. Экспериментальным путем изучены положения способа кормления свиней, как способа снижения негативного влияния стресс-факторов на качество мяса посредством изученного фактора питания

DETECTION OF AUTOANTIBODIES RECOGNIZING CANCERRETINA ANTIGEN RECOVERIN IN BLOOD OF PATIENTS WITH NON-INVASIVE FOLLICULAR THYROID NEOPLASMS WITH PAPILLARY-LIKE NUCLEAR FEATURES (NIFTP)

Autoantibodies recognizing the cancer-retina autoantigen called recoverin (RCVRN-AutoAb) may serve as a highly specific biomarker of cancer-associated retinopathy. However, they may also be found in some cancer patients without clinical evidence of retinopathy. In the present study, dot-ELISA and Western blot assays were used to demonstrate the presence of circulating RCVRN-AutoAb in 4/7 (57%) of patients with recently recognized pathological entity, non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP); other thyroid tumors represented by follicular adenomas, and classical and follicular variants of papillary thyroid carcinomas, demonstrated low frequencies of RCVRN-AutoAb (0/15, 1/20 (5%) and 1/15 (7%), respectively), with no significant differences from healthy individuals (0/15). Our data implicate the circulating RCVRN-AutoAb as a potential biomarker of NIFTP capable of discrimination of this novel pathological entity from other thyroid tumors