Distal radius fractures: a prospective study of the clinical and radiological outcomes in surgically managed patients

Background: Objective of the research was to evaluate radiological and functional outcome in fractures of the distal radius treated by various surgical methods.Methods: 89 patients with distal radius fractures were randomly divided into three groups according to a computer-generated table of randomization. 40 patients were managed by K wire fixation. 22 patients were managed by external fixator. 27 patients were managed by volar plating technique. Anatomical restoration was evaluated by posterior-anterior and lateral radiographs obtained preoperatively and at 09 months of follow up to evaluate radial height (RH), radial inclination (RI) and volar tilt (VT). Functional outcome was evaluated using Mayo scoring system.Results: According to Mayo score, in K-wire group 72.5% (n=29) patients had excellent to good outcome, 17.5% (n=7) had fair outcome while as 10% (n=4) patients had poor outcome. In external fixator group 72.7% (n=16) patients had excellent to good outcome while as 27.3% (n=6) had fair outcome. In volar plating group 81.5% (n=22) patients had excellent to good outcome while as 18.5% (n=5) had fair outcome.Conclusions: In our study we found that volar locked plating provides advantage in dorsally or volarly displaced intra-articular fractures with excellent to good radiological outcome. However, in type B (partial intra-articular fractures) fractures, volar locking compression plate (LCP) and external fixator provide almost equivocal results. Percutaneous pinning can be recommended for extra-articular or simple intra-articular fracture patterns, with a loss of volar and/or RI but without significant radial shortening

Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes

Alterations in genomic CpG methylation patterns have been found to be associated with cell transformation and neoplasia. Although it is recognized that methylation of CpG residues negatively regulates gene expression, how the various MBPs (methyl-binding proteins) contribute to this process remains elusive. To determine whether the two well characterized proteins MeCP2 (methyl-CpG-binding protein 2) and MBD1 (methyl-CpG-binding domain 1) have distinct or redundant functions, we employed RNAi (RNA interference) to silence their expression in the prostate cancer-derived PC3cell line, and subsequently compared cell growth, invasion and migration properties of these cell lines in addition to their respective mRNA-expressionprofiles. Cells devoid of MeCP2 proliferated more poorly compared with MBD1-deficient cells and the parental PC3 cells. Enhanced apoptosis was observed in MeCP2-deficient cells, whereas apoptosis in parental and MBD1-deficient cells appeared to be equivalent. Boyden chamber invasion and wound-healing migration assays showed that MBD1-silenced cells were both more invasive and migratory compared with MeCP2-silenced cells. Finally, gene chip microarray analyses showed striking differences in the mRNA-expression profiles obtained from MeCP2- and MBD1-depleted cellsrelative to each other as well as when compared with control cells. The results of the present study suggest that MeCP2 and MBD1 silencing appear to affect cellular processes independently in vivo and that discrete sets of genes involved in cellular proliferation, apoptosis, invasion and migration are targeted by each protein

The Use of DNA Profiling in Identifying Murderers and Sexual Abusers: A Case Report

A complaint was registered at a Police Station in the Faisalabad District of Pakistan. A boy aged 7-8 years went to a nearby shop and never came back. He was last seen with the accused by eyewitnesses. The naked dead body of the boy was found tied with his clothing in nearby fields. An autopsy revealed three incised wounds on the neck. Evidence material from the dead body and crime scene were submitted for forensic analysis. The evidence material was screened for human biological fluids, semen, and blood. The items positive for semen or blood were subjected to DNA extraction. Short Tandem Repeat (STR) markers were amplified from extracted DNA after quantification. The amplicons were subjected to capillary electrophoresis to generate DNA profiles. Mixture DNA profiles were interpreted by EuroForMix, the probabilistic genotyping software. Post-mortem examination indicated the possibility of sexual abuse of the boy prior to his killing. Seminal material was found on anal swabs collected from the dead body. The DNA profile obtained from anal swabs was consistent with a mixture of the DNA profiles of both the suspect and the victim. The DNA profile of the suspect was also found in nail swabs taken from the victim. The DNA profile generated from blood stains present on the suspect’s clothing matched the DNA profile of the victim. The DNA of the suspect was present on anal and nail swabs collected from the victim. The victim’s DNA was present in blood stains present on the suspect’s clothing. The suspect was confirmed to be the perpetrator

Regulation of voltage and frequency in solid oxide fuel cell-based autonomous microgrids using the Whales optimisation algorithm

This study explores the Whales Optimization Algorithm (WOA)-based PI controller for regulating the voltage and frequency of an inverter-based autonomous microgrid (MG). The MG comprises two 50 kW DGs (solid oxide fuel cells, SOFCs) interfaced using a power electronics-based voltage source inverter (VSI) with a 120-kV conventional grid. Four PI controller schemes for the MG are implemented: (i) stationary PI controller with fixed gain values (Kp and Ki), (ii) PSO tuned PI controller, (iii) GWO tuned PI controller, and (iv) WOA tuned PI controller. The performance of these controllers is evaluated by monitoring the system voltage and frequency during the transition of MG operation mode and changes in the load. The MATLAB/SIMULINK tool is utilised to design the proposed model of grid-tied MG alongside the MATLAB m-file to apply an optimisation technique. The simulation results show that the WOA-based PI controller which optimises the control parameters, achieve 62.7% and 59% better results for voltage and frequency regulation, respectively. The eigenvalue analysis is also provided to check the stability of the proposed controller. Furthermore, the proposed system also satisfies the limits specified in IEEE-1547-2003 for voltage and frequency

Search for the standard model Higgs boson in the H to ZZ to 2l 2nu channel in pp collisions at sqrt(s) = 7 TeV

A search for the standard model Higgs boson in the H to ZZ to 2l 2nu decay channel, where l = e or mu, in pp collisions at a center-of-mass energy of 7 TeV is presented. The data were collected at the LHC, with the CMS detector, and correspond to an integrated luminosity of 4.6 inverse femtobarns. No significant excess is observed above the background expectation, and upper limits are set on the Higgs boson production cross section. The presence of the standard model Higgs boson with a mass in the 270-440 GeV range is excluded at 95% confidence level.Comment: Submitted to JHE

Search for anomalous t t-bar production in the highly-boosted all-hadronic final state

A search is presented for a massive particle, generically referred to as a Z', decaying into a t t-bar pair. The search focuses on Z' resonances that are sufficiently massive to produce highly Lorentz-boosted top quarks, which yield collimated decay products that are partially or fully merged into single jets. The analysis uses new methods to analyze jet substructure, providing suppression of the non-top multijet backgrounds. The analysis is based on a data sample of proton-proton collisions at a center-of-mass energy of 7 TeV, corresponding to an integrated luminosity of 5 inverse femtobarns. Upper limits in the range of 1 pb are set on the product of the production cross section and branching fraction for a topcolor Z' modeled for several widths, as well as for a Randall--Sundrum Kaluza--Klein gluon. In addition, the results constrain any enhancement in t t-bar production beyond expectations of the standard model for t t-bar invariant masses larger than 1 TeV.Comment: Submitted to the Journal of High Energy Physics; this version includes a minor typo correction that will be submitted as an erratu

Combined search for the quarks of a sequential fourth generation

Results are presented from a search for a fourth generation of quarks produced singly or in pairs in a data set corresponding to an integrated luminosity of 5 inverse femtobarns recorded by the CMS experiment at the LHC in 2011. A novel strategy has been developed for a combined search for quarks of the up and down type in decay channels with at least one isolated muon or electron. Limits on the mass of the fourth-generation quarks and the relevant Cabibbo-Kobayashi-Maskawa matrix elements are derived in the context of a simple extension of the standard model with a sequential fourth generation of fermions. The existence of mass-degenerate fourth-generation quarks with masses below 685 GeV is excluded at 95% confidence level for minimal off-diagonal mixing between the third- and the fourth-generation quarks. With a mass difference of 25 GeV between the quark masses, the obtained limit on the masses of the fourth-generation quarks shifts by about +/- 20 GeV. These results significantly reduce the allowed parameter space for a fourth generation of fermions.Comment: Replaced with published version. Added journal reference and DO

Search for New Physics with Jets and Missing Transverse Momentum in pp collisions at sqrt(s) = 7 TeV

A search for new physics is presented based on an event signature of at least three jets accompanied by large missing transverse momentum, using a data sample corresponding to an integrated luminosity of 36 inverse picobarns collected in proton--proton collisions at sqrt(s)=7 TeV with the CMS detector at the LHC. No excess of events is observed above the expected standard model backgrounds, which are all estimated from the data. Exclusion limits are presented for the constrained minimal supersymmetric extension of the standard model. Cross section limits are also presented using simplified models with new particles decaying to an undetected particle and one or two jets