Improvement of phosphine fumigation by the use of Speedbox

Today, phosphine is turning to be a major fumigant for controlling insects in stored products. However, few limitations, such as low temperatures and relatively long exposure time, limit the phosphine use. In order to improve phosphine application, a special devise, containing a heater and a ventilator, called "Speedbox" has been developed by Detia Degesch GmbH Germany. For studying the effectiveness of phosphine fumigation using Speedbox, we have conducted two kinds of experiments: one in a fumigation room (Pilot) and other in commercial warehouse. For pilot fumigation, adults, pupae and late larvae of Sitophilus oryzae, Rhyzopertha dominica, Oryzaephilus surinamensis, Trogoderma granarium and Callosobruchus maculatus, and all stages of Tribolium castaneum Herbst, Plodia interpunctella and Ephestia cautella were used as test insects. One to three Degesch Plates (about 2-6 g of phosphine gas per m3) were used. Exposure time was 1 to 3 days. The phosphine concentrtion was monitored by Bedfont device model 415. At 4 g/m3 for 48 ha maximum of phosphine concentration of 1460 ppm was reached. The total mortality of all tested insects and stages was recorded, except the eggs of E. cautella (98%). The commercial stack fumigation was done at the dosages of 2-4 g/m3, exposure time of 2-4 days and commodity temperatures of 6-17ºC. At a target concentration of 4 g/m3, 2 hours after beginning of the treatment, the concentration of the gas has reached 414 ppm, with a maximum of 1480 ppm. The total mortality of tested insects at adult, late larvae and pupae stages was recorded. The use of Speedbox allows one-day decrease in the plates degassing time, recirculation of the gas and its event distribution in the treated space and controlling major stored product insects for shorter exposure time at low temperatures. Keywords: Fumigation; Posphine; Speedbox; Stored-product insect

Laboratory evaluation of diatomaceous earth against main stored product insects

The sensitivity of the main external and internal stored product insect pests to the commercial formulation of Detia Degesch Diatomaceous Earth – DDDE - Inerto (DE) was studied in laboratory experiments. The tested insects were adults of internal feeders Sitophilus oryzae Rhyzopertha dominica and external feeders Oryzaephilus surinamensis, Tribolium castaneum, and larvae (third instar) of T.castaneum. The DE was applied to wheat grain of 12% moisture content at concentrations of 0.5, 1.0, 2.0 and 4.0 g/kg of grain. The treated and untreated (control) grain were kept at 28°C and 65 ± 5% r.h. The numbers of dead and survived insects were counted two, three and four weeks after treatment. The number of adult progeny was counted nine weeks after treatment. At a concentration of 0.5 g/kg, mortality of S. oryzae and O. surinamensis after three weeks of exposure to DE were 92 and 86%, respectively. In contrast, mortality of T. castaneum and R. dominica adults was 3 and 37%, respectively. Progeny production of O. surinamensis and T. castaneum at a concentration of 2 g/kg was negligible, since only few individuals were recorded nine weeks after treatment, in comparison with the high progeny production in the control grain. The progeny of S. oryzae was also reduced. In contrast, for R. dominica was reduced only twice, in comparison with the control. In the case of T. castaneum larvae, at a concentration of 2 g/kg, after 4 weeks of exposure, 37% of the larvae emerged to adults, compared with 95% in control. Nine weeks after treatment, the number of F1adults was 100% suppressed. DE efficacy was similar at 4 g/kg. Based on the findings of the present study, the efficacy of the tested DE was influenced by DE concentration, insect species, developmental stage and exposure interval to the treated commodity.Keywords: Diatomaceous earth, Stored product insects, Wheat grai