ISSR Analysis of Variability of Cultivated Form and Varieties of Pomegranate (Punica granatum L.) from Azerbaijan

The article presents the results of a study of genetic polymorphism for the first time carried out on pomegranate varieties and forms of Azerbaijan origin using molecular markers. In total, 102 PCR fragments were identified, of which 80 were polymorphic. The high level of polymorphism (75.5%) and the rich genetic diversity were identified among the studied pomegranate collection. As a result of data analysis and on the basis of the values of the basic parameters (PIC, EMR, MI, RP, MRP) determining informativeness of markers, all 14 ISSR primers were suitable for genotyping pomegranate accessions. The most effective markers (UBC808, UBC811, UBC834, and UBC840) were identified among the set of primers tested. A dendrogram was constructed on the basis of the data obtained, which made it possible to group genotypes into 16 major clusters. The genetic similarity index ranged from 0.032 to 0.94. The study of the genetic relationship of different pomegranate varieties confirms the effectiveness of the ISSR method, which makes it possible to determine the level of genetic diversity, as well as to establish the relationship among the studied pomegranate accessions

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18–49, 50–69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population

The investigation of the transient regimes in the nonlinear systems by the generalized classical method

This paper presents the use of the generalized classical method (GCM) for solving linear and nonlinear differential equations. This method is based on the differential transformation (DT) technique. In the GCM, the solution of the nonlinear transient regimes in the physical processes can be written as a functional series with unknown coefficients. The series can be chosen to satisfy the initial and boundary conditions which represent the properties of the physical process. The unknown coefficients of the series are determined from the differential transformation of the nonlinear differential equation of the system. Therefore, the approximate solution of the nonlinear differential equation can be obtained as a closed-form series

Клініко-гемодинамічний ефект фруктозо-1,6-дифосфату и фосфокреатину у хворих на гострий коронарний синдром

One of the promising areas of treating cardiovascular diseases is the use of drugs with metabolic action. Metabolic drugs have not only the ability to improve myocardial energy metabolism, but also strong antioxidant properties.Одним из перспективных направлений терапии сердечно-сосудистых заболеваний является применение препаратов с метаболическим действием. Метаболические препараты обладают не только способностью улучшать энергетический обмен миокарда, но и выраженными антиоксидантными свойствами.Одним із перспективних напрямків терапії серцево-судинних захворювань є застосування препаратів метаболічної дії. Метаболічні препарати володіють не лише здатністю покращувати енергетичний обмін міокарда, але й мають виражені антиоксидантні властивості