1,231 research outputs found
Structure and Metal Binding Properties of ZnuA, a Periplasmic Zinc Transporter from \u3cem\u3eEscherichia coli\u3c/em\u3e
ZnuA is the periplasmic Zn2+-binding protein associated with the high-affinity ATP-binding cassette ZnuABC transporter from Escherichia coli. Although several structures of ZnuA and its homologs have been determined, details regarding metal ion stoichiometry, affinity, and specificity as well as the mechanism of metal uptake and transfer remain unclear. The crystal structures of E. coli ZnuA (Eco-ZnuA) in the apo, Zn2+-bound, and Co2+-bound forms have been determined. ZnZnuA binds at least two metal ions. The first, observed previously in other structures, is coordinated tetrahedrally by Glu59, His60, His143, and His207. Replacement of Zn2+ with Co2+ results in almost identical coordination geometry at this site. The second metal binding site involves His224 and several yet to be identified residues from the His-rich loop that is unique to Zn2+ periplasmic metal binding receptors. Electron paramagnetic resonance and X-ray absorption spectroscopic data on CoZnuA provide additional insight into possible residues involved in this second site. The second site is also detected by metal analysis and circular dichroism (CD) titrations. Eco-ZnuA binds Zn2+ (estimated K d \u3c 20 nM), Co2+, Ni2+, Cu2+, Cu+, and Cd2+, but not Mn2+. Finally, conformational changes upon metal binding observed in the crystal structures together with fluorescence and CD data indicate that only Zn2+ substantially stabilizes ZnuA and might facilitate recognition of ZnuB and subsequent metal transfer
A Precision Angle Sensor using an Optical Lever inside a Sagnac Interferometer
We built an ultra low noise angle sensor by combining a folded optical lever
and a Sagnac interferometer. The instrument has a measured noise floor of 1.3
prad / Hz^(1/2) at 2.4 kHz. We achieve this record angle sensitivity using a
proof-of-concept apparatus with a conservative N=11 bounces in the optical
lever. This technique could be extended to reach sub-picoradian / Hz^(1/2)
sensitivities with an optimized design.Comment: 3 pages, 4 figure
Perioperative intraperitoneal chemotherapy for peritoneal surface malignancy
The treatment of peritoneal surface malignancy mainly focuses on diffuse malignant peritoneal mesothelioma, pseudomyxoma peritonei from appendiceal cancer, and peritoneal dissemination from gastrointestinal and ovarian cancers. Cancer progression causes peritoneal implants to be distributed throughout the abdominopelvic cavity. These nodules plus the ascitic fluid result in abdominal distension. As the disease progresses, these tumors cause intestinal obstruction leading to debilitating symptoms and a greatly impaired quality of life. In the past, the prognosis of patients with peritoneal surface malignancy was regarded dismal and cure was not an option. Recently, cytoreductive surgery combined with perioperative intraperitoneal chemotherapy has shown an improved survival in selected patients with this disease. To date, multiple different treatment regimens of perioperative intraperitoneal chemotherapy have been used. This review focuses on the perioperative intraperitoneal chemotherapy currently in use in conjunction with cytoreductive surgery for the treatment of peritoneal surface malignancy at the Washington Cancer Institute
Heated IntraPEritoneal Chemotherapy (HIPEC) for Patients With Recurrent Ovarian Cancer A Systematic Literature Review
Mass measurements near the -process path using the Canadian Penning Trap mass spectrometer
The masses of 40 neutron-rich nuclides from Z = 51 to 64 were measured at an
average precision of using the Canadian Penning Trap mass
spectrometer at Argonne National Laboratory. The measurements, of fission
fragments from a Cf spontaneous fission source in a helium gas catcher,
approach the predicted path of the astrophysical process. Where overlap
exists, this data set is largely consistent with previous measurements from
Penning traps, storage rings, and reaction energetics, but large systematic
deviations are apparent in -endpoint measurements. Differences in mass
excess from the 2003 Atomic Mass Evaluation of up to 400 keV are seen, as well
as systematic disagreement with various mass models.Comment: 15 pages, 16 figures. v2 updated, published in Physical Review
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