Helping At Risk Women Transition Back Home

An expanding movement within higher education has attempted to make universities more relevant and responsive to the communities and states in which they are located, utilizing community-based partnerships to enhance student service learning opportunities and strengthen their own communities. These partnerships provide a mechanism by which underserved populations might receive more attention. This article documents Eastern Kentucky University’s partnership with a community-based agency that serves low-income individuals and families in central Kentucky counties to improve the lives and confidence of women involved with the criminal justice system by offering them coping skills, tools and resources that will help them to view themselves as valued members of the community

Poster 317: Do OnabotulinumtoxinA Injections Affect Seizure Threshold in Children? A Report on 2 Cases

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/147122/1/pmr2s140a.pd

Deep Blue Light Emission of (4,3-Oxo?HCMM) Coumarin Derivative for Organic Led Display Application

In the present technological world huge demand of white light emitting diodes has received much importance due to their vast applications in various sensors, lightning devices and display etc. The white light can be produced by mixing exact amount of green, blue and red light. Therefore, we made an attempt to produce a LED application making use of organic compounds. Hence, we synthesized 4-Hydroxy-3-[(4-Hydroxy-2-Oxo-2H-Chromen-3-yl) (4-Methoxyphenyl) Methyl]-2H-Chromen-2-One (4,3-OxoHCMM). In this present work we report, the 4,3-OxoHCMM possesses high color purity, good CIE chromaticity coordinate, and they would have potential organic light emitting devices (LED) application, this simple method to produce the blue light as blue component can play important role in WLED. Uv-Vis absorption spectra is used to determine the optical energy bandgap Eg, andfrom the photoluminescence spectra duality nature of wavelengths for 4,3-OxoHCMM molecule in different solvents is obtained due to solvation effect, this result shows a simple extraction of dye in different solvents which can be used to produce the desired wavelength

Predicting Graph Categories from Structural Properties

Complex networks are often categorized according to the underlying phenomena that they represent such as molecular interactions, re-tweets, and brain activity. In this work, we investigate the problem of predicting the category (domain) of arbitrary networks. This includes complex networks from different domains as well as synthetically generated graphs from five different network models. A classification accuracy of 96.6% is achieved using a random forest classifier with both real and synthetic networks. This work makes two important findings. First, our results indicate that complex networks from various domains have distinct structural properties that allow us to predict with high accuracy the category of a new previously unseen network. Second, synthetic graphs are trivial to classify as the classification model can predict with near-certainty the network model used to generate it. Overall, the results demonstrate that networks drawn from different domains (and network models) are trivial to distinguish using only a handful of simple structural properties

A kinetic model of TBP auto-regulation exhibits bistability

<p>Abstract</p> <p>Background</p> <p>TATA Binding Protein (TBP) is required for transcription initiation by all three eukaryotic RNA polymerases. It participates in transcriptional initiation at the majority of eukaryotic gene promoters, either by direct association to the TATA box upstream of the transcription start site or by indirectly localizing to the promoter through other proteins. TBP exists in solution in a dimeric form but binds to DNA as a monomer. Here, we present the first mathematical model for auto-catalytic TBP expression and use it to study the role of dimerization in maintaining the steady state TBP level.</p> <p>Results</p> <p>We show that the autogenous regulation of TBP results in a system that is capable of exhibiting three steady states: an unstable low TBP state, one stable state corresponding to a physiological TBP concentration, and another stable steady state corresponding to unviable cells where no TBP is expressed. Our model predicts that a basal level of TBP is required to establish the transcription of the TBP gene, and hence for cell viability. It also predicts that, for the condition corresponding to a typical mammalian cell, the high-TBP state and cell viability is sensitive to variation in DNA binding strength. We use the model to explore the effect of the dimer in buffering the response to changes in TBP levels, and show that for some physiological conditions the dimer is not important in buffering against perturbations.</p> <p>Conclusions</p> <p>Results on the necessity of a minimum basal TBP level support the in vivo observations that TBP is maternally inherited, providing the small amount of TBP required to establish its ubiquitous expression. The model shows that the system is sensitive to variations in parameters indicating that it is vulnerable to mutations in TBP. A reduction in TBP-DNA binding constant can lead the system to a regime where the unviable state is the only steady state. Contrary to the current hypotheses, we show that under some physiological conditions the dimer is not very important in restoring the system to steady state. This model demonstrates the use of mathematical modelling to investigate system behaviour and generate hypotheses governing the dynamics of such nonlinear biological systems.</p> <p>Reviewers</p> <p>This article was reviewed by Tomasz Lipniacki, James Faeder and Anna Marciniak-Czochra.</p

Frequency of primiparous mother initiated breastfeeding earlier after birth and factors associated with it in Sindh, Pakistan: A secondary analysis

Background: Initiation of breastfeeding within one hour after delivery plays a vital role in a newborns life by not only increasing their survival rate but also reducing many life-threatening diseases in the newborn. The aim of the study was to determine frequency of early initiation of breastfeeding among primiparous mothers in a rural district of Thatta, Pakistan.Methods: This study was conducted using survey data extracted from a cross-sectional study conducted in 2019 on prevalence of exclusive breastfeeding and factors associated with it among women in Thatta. The outcome was early initiation of breastfeeding. To determine factors associated with it, multi variable logistic regression was carried out.Results: The study showed that 30.3% of primiparous mothers in Thatta initiated breastfeeding within one hour of birth and knowledge of mothers (OR=9.76, 95% CI: 1.99-17.59), place of birth (OR=3.51, 95% CI: 1.32-9.31) and support of health care professional at health facility (OR=2.93, 95% CI: 1.09-7.86) are the factors significantly associated with early initiation of breastfeeding among primiparous mothers.Conclusions: In order to enhance the early initiation of breastfeeding, it is important for health care professionals to emphasize on the effect of pre-lacteal feeding during and support breastfeeding immediately after delivery, especially among women who had given birth for the first time

Genetic separation of Sae2 nuclease activity from Mre11 nuclease functions in budding yeast

Sae2 promotes the repair of DNA double-strand breaks in Saccharomyces cerevisiae. The role of Sae2 is linked to the Mre11/Rad50/Xrs2 (MRX) complex, which is important for the processing of DNA ends into single-stranded substrates for homologous recombination. Sae2 has intrinsic endonuclease activity, but the role of this activity has not been assessed independently from its functions in promoting Mre11 nuclease activity. Here we identify and characterize separation-of-function mutants that lack intrinsic nuclease activity or the ability to promote Mre11 endonucleolytic activity. We find that the ability of Sae2 to promote MRX nuclease functions is important for DNA damage survival, particularly in the absence of Dna2 nuclease activity. In contrast, Sae2 nuclease activity is essential for DNA repair when Mre11 nuclease is compromised. Resection of DNA breaks is impaired when either Sae2 activity is blocked, suggesting roles for both Mre11 and Sae2 nuclease activities in promoting the processing of DNA ends in vivo. Finally, both activities of Sae2 are important for sporulation, indicating that the processing of meiotic breaks requires both Mre11 and Sae2 nuclease activities

Validation of EpiTRAQ, a transition readiness assessment tool for adolescents and young adults with epilepsy

ObjectiveTo design and validate a transition readiness assessment tool for adolescents and young adults with epilepsy and without intellectual disability.MethodsWe adapted a general transition readiness assessment tool (TRAQ) to add epilepsy‐relevant items based on concepts in current epilepsy quality measures. The adapted tool, EpiTRAQ, maintained the original structure and scoring system. Concurrent with clinical implementation in pediatric and adult epilepsy clinics at an academic medical center, we assessed the validity and reliability of this adapted tool for patients 16‐26 years of age. This process included initial validation with 302 patients who completed EpiTRAQ between October 2017 and May 2018; repeat validation with 381 patients who completed EpiTRAQ between June 2018 and September 2019; and retest reliability among 153 patients with more than one completed EpiTRAQ.ResultsMean scores were comparable between initial and repeat validation populations (absolute value differences between 0.05 and 0.1); internal consistency ranged from good to high. For both the initial and repeat validation, mean scores and internal consistency demonstrated high comparability to the original TRAQ validation results. Upon retest, few patients rated themselves with a lower score, while the majority rated themselves with higher scores.SignificanceEpiTRAQ is a valid and reliable tool for assessing transition readiness in adolescents and young adults with epilepsy and without intellectual disability.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/162789/2/epi412427_am.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/162789/1/epi412427.pd

RNA sequencing of cancer reveals novel splicing alterations

Breast cancer transcriptome acquires a myriad of regulation changes, and splicing is critical for the cell to “tailor-make” specific functional transcripts. We systematically revealed splicing signatures of the three most common types of breast tumors using RNA sequencing: TNBC, non-TNBC and HER2-positive breast cancer. We discovered subtype specific differentially spliced genes and splice isoforms not previously recognized in human transcriptome. Further, we showed that exon skip and intron retention are predominant splice events in breast cancer. In addition, we found that differential expression of primary transcripts and promoter switching are significantly deregulated in breast cancer compared to normal breast. We validated the presence of novel hybrid isoforms of critical molecules like CDK4, LARP1, ADD3, and PHLPP2. Our study provides the first comprehensive portrait of transcriptional and splicing signatures specific to breast cancer sub-types, as well as previously unknown transcripts that prompt the need for complete annotation of tissue and disease specific transcriptome