Improving the efficacy of potato clonal micropropagation by inoculation with the rhizosphere bacteria <i>Azospirillum baldaniorum</i> Sp245 and <i>Ochrobactrum cytisi</i> IPA7.2

Sustainable development of agriculture depends on the provision of quality seeds to the market. Inoculation with plant-growth-promoting rhizobacteria in in vitro culture can be used to improve the growth efficacy and performance of microplants. We examined the effect of in vitro inoculation of microplants of the cultivars Nevsky and Kondor with the strains Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2 separately and in combination. We examined the morphological variables of plant growth in in vitro culture and under ex vitro adaptation conditions; we also investigated the growth and performance of the plants in the greenhouse. The dependence of the inoculation eff icacy on potato genotype, growth stage, and inoculum composition was ascertained throughout the experiment. In vitro, A. baldaniorum Sp245 alone and in combination with O. cytisi IPA7.2 promoted the formation of roots on the microplants of both cultivars and the growth of Nevsky shoots. During plant growth ex vitro, all growth variables of the Nevsky microplants were promoted by O. cytisi IPA7.2 alone and in combination with A. baldaniorum Sp245. In both cultivars grown in the greenhouse, shoot growth was promoted in most inoculation treatments. The survival ability of the Nevsky microplants in the greenhouse increased 1.7-fold under the effect of simultaneous inoculation. Inoculation of microplants with a combination of A. baldaniorum Sp245 and O. cytisi IPA7.2 increased the number of Nevsky minitubers 1.5-fold and the number of Kondor minitubers 3.5-fold. Inoculation with the tested strains can be used to promote the growth of microplants and increase the yield of minitubers in potato seed breeding for the production of healthy planting material

Рефрактерный эпилептический статус после клипирования аневризмы средней мозговой артерии (клиническое наблюдение)

We present the management features for the refractory epileptic status in a patient after surgical treatment of unruptured cerebral aneurysm and no epileptic prehistory. The role of continuous electroencephalographic monitoring in adjusting the rate of drugs administration for general anesthesia in the treatment of this condition is also described.Приведена демонстрация и обсуждение особенностей ведения пациентки с рефрактерным эпилептическим статусом после хирургического лечения неразорвавшейся аневризмы головного мозга без эпилептического анамнеза. Показана роль непрерывного электроэнцефалографического мониторинга для подбора скорости введения препаратов для общей анестезии при лечении данного состояния

Проведение неинвазивной вспомогательной вентиляции легких в ходе санитарно-авиационной эвакуации у пациента с тяжелой внебольничной пневмонией

We report the experience of sanitary aviation evacuation of a patient with severe respiratory failure on the background of community-acquired pneumonia using mask non-invasive ventilation. The use of this method of ventilation of the lungs made it possible to avoid undesirable consequences arising from the transfer of the patient to artificial ventilation of the lungs and to transport him safely to a specialized medical institution in order to continue treatment. The described method of preparing a patient with respiratory failure before aviation transportation has shown its effectiveness during the flight and may be recommended for use by airmobile crews when carrying out long-distance evacuationПредставлен опыт санитарной авиационной эвакуации на дальнее расстояние пациента с тяжелой дыхательной недостаточностью на фоне внебольничной пневмонии с применением масочной неинвазивной вспомогательной вентиляции легких. Использование данного способа вентиляции легких позволило избежать нежелательных последствий, возникающих при переводе пациента на искусственную вентиляцию легких, и безопасно осуществить транспортировку в специализированное медицинское учреждение для продолжения лечения. Описываемый способ подготовки пациента с дыхательной недостаточностью перед авиационной транспортировкой показал свою эффективность во время полета и может быть рекомендован для использования аэромобильными бригадами при осуществлении эвакуации на дальние расстояния

Refractory Status Epilepticus After the Middle Cerebral Artery Aneurysm Clipping (a Case Report)

We present the management features for the refractory epileptic status in a patient after surgical treatment of unruptured cerebral aneurysm and no epileptic prehistory. The role of continuous electroencephalographic monitoring in adjusting the rate of drugs administration for general anesthesia in the treatment of this condition is also described

Non-invasive Ventilation During Sanitary Aircraft Evacuation in a Patient with Severe Community-acquired Pneumonia

We report the experience of sanitary aviation evacuation of a patient with severe respiratory failure on the background of community-acquired pneumonia using mask non-invasive ventilation. The use of this method of ventilation of the lungs made it possible to avoid undesirable consequences arising from the transfer of the patient to artificial ventilation of the lungs and to transport him safely to a specialized medical institution in order to continue treatment. The described method of preparing a patient with respiratory failure before aviation transportation has shown its effectiveness during the flight and may be recommended for use by airmobile crews when carrying out long-distance evacuatio

Influence of Physical and Chemical Modification on the Optical Rotatory Dispersion and Biological Activity of Chitosan Films

The optical and bactericidal properties of acetic and basic chitosan films were studied. By the ORD technique, we found that these films differed in the values of their specific optical rotation and of their rotary and dispersive constants. A sign inversion of was observed when the acetic chitosan films were heat-treated. The bactericidal activity of the initial and dehydrated acetic films was analyzed, and their moisture content and optical and biological activities were compared

The impact of transesophageal echocardiography based protocol for management of adults in the sitting position on the incidence of clinically significant venous air embolism

The purpose of the study was to evaluate the impact of transesophageal echocardiography (TEE) – based protocol for management of adults in the sitting position during elective neurosurgical interventions on the incidence of clinically significant venous air embolism (VAE). Material and Methods ― The study involved 155 adult neurosurgery patients (70 in prospective group and 85 in retrospective group). Surgery in both groups was done in the sitting position. In the prospective group TEE-based protocol was used. Retrospective group served as control. The primary endpoint was considered to be a decrease in the frequency of clinically significant VAE in the prospective group in comparison with the retrospective one. In the prospective group, VAE with Tuebingen grade 3-5 was considered clinically significant. The PFO incidence and severity and the effect of the number of episodes of VAE per case on its maximum severity during surgery were also analyzed. Any complication in the postoperative period believed to be associated with the position of the patient on the table during the surgery was recorded. Results ― The incidence of the clinically significant VAE in the retrospective group was 23.5% (95% CI 15-34) and was 16.4% higher than the frequency in the prospective group (chi-square=7.6197, df=1, p=0.005). 50% (95% CI 38-62) of patients in prospective group developed VAE during surgery. In 16 cases, the number of episodes was more than one. The number of episodes of VAE in the observation was reliably associated with the maximum severity of VAE during the observation (Z=4.11; p<0.001). A moderate strength relationship was determined between them (SomersDelta=0.43; 95% CI 0.17-0.7). Not a single case of paradoxical air embolism was detected in a series of observations. None of the patients has got a neurological deficit or cardiopulmonary complications associated with the position on the surgical table in the postoperative period. Pneumocephalus was found in 100% of cases on head computed tomography, which, however, did not need surgical treatment. PFO in the prospective group was detected in 62% (95% CI 52-73) of patients. In 25% (95% CI 16-35), shunting was significant. A large PFO without Valsalva maneuver was detected in 12.5% (95% CI 6-21) of cases. Conclusion ― The use of the TEE-based protocol for the management of adult patients in a sitting position during elective neurosurgical interventions can reduce the incidence of clinically significant VAE