Regional Responses: The Sustainable Food North West Research Collaboration

This paper describes the conception and initial phase of the North West Sustainable Food Research Collaboration (SusfoodNW). It provides summary findings from exploratory work packages which commenced at the beginning of the initiative and feedback from regional stakeholders concerning the role and challenges for regional academic collaborations and sustainable food research more broadly. As such, it is a collection of reflections and research outputs that aim to illustrate: a) The process of academics from neighbouring institutions working together with a regional focus but without significant external resource b) Some findings from initial research on the region which aimed to map and understand some of the context for sustainable food in the region and provide a grounding for further research c) A snapshot of regional stakeholder opinion in relation to the focus and activities of a regional research collaboratio

Suitability of Killai backwaters for prawn farming-a preliminary micro level survey

Brackishwater areas have been given much importance for prawn farming. No information was available on the Killai backwaters about factors like water quality, topography, contour, extent of the area, tidal amplitude, seed potential and possibilities of flooding etc. Hence during 1982-'84 Klllai area was thoroughly surveyed on the above aspects and the results have been discussed in this paper. From this it is inferred that a total area of about 155 ha is readily available for undertaking both pond and pen culture in this backwater

Next-generation sequencing identifies the natural killer cell microRNA transcriptome

Natural killer (NK) cells are innate lymphocytes important for early host defense against infectious pathogens and surveillance against malignant transformation. Resting murine NK cells regulate the translation of effector molecule mRNAs (e.g., granzyme B, GzmB) through unclear molecular mechanisms. MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate the translation of their mRNA targets, and are therefore candidates for mediating this control process. While the expression and importance of miRNAs in T and B lymphocytes have been established, little is known about miRNAs in NK cells. Here, we used two next-generation sequencing (NGS) platforms to define the miRNA transcriptomes of resting and cytokine-activated primary murine NK cells, with confirmation by quantitative real-time PCR (qRT-PCR) and microarrays. We delineate a bioinformatics analysis pipeline that identified 302 known and 21 novel mature miRNAs from sequences obtained from NK cell small RNA libraries. These miRNAs are expressed over a broad range and exhibit isomiR complexity, and a subset is differentially expressed following cytokine activation. Using these miRNA NGS data, miR-223 was identified as a mature miRNA present in resting NK cells with decreased expression following cytokine activation. Furthermore, we demonstrate that miR-223 specifically targets the 3′ untranslated region of murine GzmB in vitro, indicating that this miRNA may contribute to control of GzmB translation in resting NK cells. Thus, the sequenced NK cell miRNA transcriptome provides a valuable framework for further elucidation of miRNA expression and function in NK cell biology

Sequencing technologies and genome sequencing

The high-throughput - next generation sequencing (HT-NGS) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the Sanger method. With the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cost of $1,000 each is seemed to be realistically feasible in the near future. In the relatively short time frame since 2005, the HT-NGS technologies are revolutionizing the human and animal genome researches by analysis of chromatin immunoprecipitation coupled to DNA microarray (ChIP-chip) or sequencing (ChIP-seq), RNA sequencing (RNA-seq), whole genome genotyping, genome wide structural variation, de novo assembling and re-assembling of genome, mutation detection and carrier screening, detection of inherited disorders and complex human diseases, DNA library preparation, paired ends and genomic captures, sequencing of mitochondrial genome and personal genomics. In this review, we addressed the important features of HT-NGS like, first generation DNA sequencers, birth of HT-NGS, second generation HT-NGS platforms, third generation HT-NGS platforms: including single molecule Heliscope™, SMRT™ and RNAP sequencers, Nanopore, Archon Genomics X PRIZE foundation, comparison of second and third HT-NGS platforms, applications, advances and future perspectives of sequencing technologies on human and animal genome research

Tiered UoG Smart Campus Architectures

The UoG is partway through a ten-year campus upgrade programme and a key goal is to embed smart sensing infrastructure into the new physical fabric. As a prototyping exercise, we use modest commodity sensor nodes (i.e. Raspberry Pis & Wemos Board Microcontrollers) and low-cost, low-precision sensors for indoor environmental monitoring. This bundle includes the tiered architectures that include PRT, PWT, PRS & PWS