A Report on the Preliminary design of Composite Cocured LCA Fin

A report on the preliminary design of composite cocured LCA fin is presented. A six spar structural configuration involving laminated carbon composite construction is employed in the design of torsion box. The design studies are carried out using a strength of materials based analysis. Three critical loading cases have been considered in the investigation. Results for various cases studied are presented and discussed

The relaxation of OH (v = 1) and OD (v = 1) by H2O and D2O at temperatures from 251 to 390 K

We report rate coefficients for the relaxation of OH(v = 1) and OD(v = 1) by H2O and D2O as a function of temperature between 251 and 390 K. All four rate coefficients exhibit a negative dependence on temperature. In Arrhenius form, the rate coefficients for relaxation (in units of 10–12 cm3 molecule–1 s–1) can be expressed as: for OH(v = 1) + H2O between 263 and 390 K: k = (2.4 ± 0.9) exp((460 ± 115)/T); for OH(v = 1) + D2O between 256 and 371 K: k = (0.49 ± 0.16) exp((610 ± 90)/T); for OD(v = 1) + H2O between 251 and 371 K: k = (0.92 ± 0.16) exp((485 ± 48)/T); for OD(v = 1) + D2O between 253 and 366 K: k = (2.57 ± 0.09) exp((342 ± 10)/T). Rate coefficients at (297 ± 1 K) are also reported for the relaxation of OH(v = 2) by D2O and the relaxation of OD(v = 2) by H2O and D2O. The results are discussed in terms of a mechanism involving the formation of hydrogen-bonded complexes in which intramolecular vibrational energy redistribution can occur at rates competitive with re-dissociation to the initial collision partners in their original vibrational states. New ab initio calculations on the H2O–HO system have been performed which, inter alia, yield vibrational frequencies for all four complexes: H2O–HO, D2O–HO, H2O–DO and D2O–DO. These data are then employed, adapting a formalism due to Troe (J. Troe, J. Chem. Phys., 1977, 66, 4758), in order to estimate the rates of intramolecular energy transfer from the OH (OD) vibration to other modes in the complexes in order to explain the measured relaxation rates—assuming that relaxation proceeds via the hydrogen-bonded complexes

DNA binding proteins of rat thigh muscle: purification and characterization of an endonuclease

Two major DNA binding proteins of molecular weights 34,000 and 38,000 have been identified in the 30,000 g supernatant (S-30) fraction of rat thigh muscle extracts. The presence of 38 KD DNA binding protein in the muscle S-30 could be demonstrated only if Triton X-100 treated extracts were used for Afinity chromatography suggesting that this protein may be a membrane associated DNA binding protein. The 38 KD DNA binding protein differed from the 34 KD DNA binding protein also in its chromatographic behaviour in DE-52 columns in which the 38 KD protein was retained, while the 34 KD protein came out in the flow-through in an electrophoretically pure form. The 34 KD DNA binding protein can also be purified by precipitation with MgCl2. Incubation of 0 · 15 M NaCl eluates (containing the 38 KD and/or 34 KD DNA binding protein) in the presence of 100 mM Mg2+ resulted in the specific precipitation of the 34 KD protein. Prolonged incubation (30 days) of the 0 · 15 Ì NaCl eluates containing the two DNA binding proteins at 4°C led to the preferential degradation of the 34 KD DNA binding protein. Nitrocellulose filter binding assays indicated selective binding of purified 34 KD protein to ss DNA. Purified 34 KD DNA binding protein cleaved pBR 322 supercoiled DNA, and electrophoresis of the cleavage products in agarose gels revealed a major DNA band corresponding to the circular form of DNA

Resistance to simian immunodeficiency virus low dose rectal challenge is associated with higher constitutive TRIM5α expression in PBMC

Background: At least six host-encoded restriction factors (RFs), APOBEC3G, TRIM5α, tetherin, SAMHD1, schlafen 11, and Mx2 have now been shown to inhibit HIV and/or SIV replication in vitro. To determine their role in vivo in the resistance of macaques to mucosally-acquired SIV, we quantified both pre-exposure (basal) and post-exposure mRNA levels of these RFs, Mx1, and IFNγ in PBMC, lymph nodes, and duodenum of rhesus macaques undergoing weekly low dose rectal exposures to the primary isolate, SIV/DeltaB670. Results: Repetitive challenge divided the monkeys into two groups with respect to their susceptibility to infection: highly susceptible (2–3 challenges, 5 monkeys) and poorly susceptible (≥6 challenges, 3 monkeys). Basal RF and Mx1 expression varied among the three tissues examined, with the lowest expression generally detected in duodenal tissues, and the highest observed in PBMC. The one exception was A3G whose basal expression was greatest in lymph nodes. Importantly, significantly higher basal expression of TRIM5α and Mx1 was observed in PBMC of animals more resistant to mucosal infection. Moreover, individual TRIM5α levels were stable throughout a year prior to infection. Post-exposure induction of these genes was also observed after virus appearance in plasma, with elevated levels in PBMC and duodenum transiently occurring 7–10 days post infection. They did not appear to have an effect on control of viremia. Interestingly, minimal to no induction was observed in the resistant animal that became an elite controller. Conclusions: These results suggest that constitutively expressed TRIM5α appears to play a greater role in restricting mucosal transmission of SIV than that associated with type I interferon induction following virus entry. Surprisingly, this association was not observed with the other RFs. The higher basal expression of TRIM5α observed in PBMC than in duodenal tissues emphasizes the understated role of the second barrier to systemic infection involving the transport of virus from the mucosal compartment to the blood. Together, these observations provide a strong incentive for a more comprehensive examination of the intrinsic, variable control of constitutive expression of these genes in the sexual transmission of HIV

4,5-Bis(1H-imidazol-1-ylmeth­yl)acridine monohydrate

In the title compound, C21H17N5·H2O, the dihedral angles between the acridine ring system and the imidazole rings are 78.8 (1) and 71.2 (1)°. The crystal packing is stabilized by O—H⋯N, C—H⋯O, C—H⋯π and π–π inter­actions [centroid–centroid separations = 3.732 (1) and 3.569 (1) Å]

Aerosol perturbations related to volcanic eruptions during the past few centuries as recorded in an ice core from the Central Dronning Maud Land, Antarctica

High-resolution glacio-chemical analyses were carried out in a 62.2 m long ice core recovered from the Central Dronning Maud Land, East Antarctica, to evaluate temporal changes in environmental characteristics during the past five centuries. The sulphate (SO 4 2-) content within the core varied between 15 and 377 ppb, whereas the sodium (Na +) and chloride (Cl -) content fluctuated widely from 7 to 500 ppb and 25 to 1461 ppb respectively. The computed non-sea-salt sulphate (nssSO 4 2-) record reveals the existence of several outstanding peaks that can be attributed to the sulphate aerosol deposition during large volcanic events as recorded in Antarctica and elsewhere. Major volcanic events identified at the core site include: Mt Pinatubo (1991), Agung (1963), Krakatau/Tarawera (1883/1886), Tambora (1815) and Huaynaputina (1600) among several other moderate events. Comparison of our nssSO 4 2- record with that of ice core records from Greenland reveals analogous sulphate deposition during certain major volcanic events, demonstrating the interhemispheric transport of aerosols during large volcanic eruptions. Relatively high Cl -/Na + ratio throughout the core (mean 3.1) compared to that of the sea water ratio implies additional sources of Cl -, most likely due to the intense scavenging of gaseous Cl - in the lower atmosphere

Simulation of the Performance of the IISc Chemical Kinetics Shock Tube

This report presents the results of an investigation of the performance of the Chemical Kinetics Shock tube at the Indian Institute of Science. The one-dimensional Lagrangian code L1d of Jacobs (1998) has been used to simulate the tube at several operating conditions. The conditions have different shock tube filling pressures, resulting in different shock speeds and different tube lengths, resulting in different dwell times. The simulations have been performed both with and without viscous effects simulated in the tubes. At the lowest shock tube filling pressure condition, the shock tube operates in an overtailored mode and it is undertailored at the higher filling pressure conditions. The results show that viscous effects, which lead to attenuation of the primary shock and heat loss from the test gas to the tube walls, result in an increasing p5 pressure during the test time. The viscous effects are more dominant at the condition with the lowest filling pressure (highest primary shock speed). A simulation run for 50 ms after diaphragm rupture or the configuration with a long driver tube shows that the test gas is periodically re-compressed by reflections of waves along the driver and shock tubes. The recompressions become sequentially weaker and thus the test gas temperature and pressure are never raised to as high levels as for the primary compression

Olanzapine-Induced Methylation Alters Cadherin Gene Families and Associated Pathways Implicated in Psychosis

BACKGROUND: The complex aetiology of most mental disorders involves gene-environment interactions that may operate using epigenetic mechanisms particularly DNA methylation. It may explain many of the features seen in mental disorders including transmission, expression and antipsychotic treatment responses. This report deals with the assessment of DNA methylation in response to an antipsychotic drug (olanzapine) on brain (cerebellum and hippocampus), and liver as a non-neural reference in a rat model. The study focuses on the Cadherin/protocadherins encoded by a multi-gene family that serve as adhesion molecules and are involved in cell-cell communication in the mammalian brain. A number of these molecules have been implicated in the causation of schizophrenia and related disorders. RESULTS: The results show that olanzapine causes changes in DNA methylation, most specific to the promoter region of specific genes. This response is tissue specific and involves a number of cadherin genes, particularly in cerebellum. Also, the genes identified have led to the identification of several pathways significantly affected by DNA methylation in cerebellum, hippocampus and liver. These included the Gα12/13 Signalling (p = 9.2E-08) and Wnt signalling (p = 0.01) pathways as contributors to psychosis that is based on its responsiveness to antipsychotics used in its treatment. CONCLUSION: The results suggest that DNA methylation changes on the promoter regions of the Cadherin/protocadherin genes impact the response of olanzapine treatment. These impacts have been revealed through the identified pathways and particularly in the identification of pathways that have been previously implicated in psychosis