Quercetin and histamine effects on free radical reactions in rat erythrocytes

The effects of quercetin and histamine separately or in combination on the free radical state of rat erythrocytes were estimated in vitro. Quercetin (0.1; 0.5; 3.0; 5.0 mM) or histamine (0.01; 10.0 μM) were added to whole blood separately or in combination. The content of hydroperoxides, TBA-active products and carbonyl groups of proteins in erythrocytes after hemolysis was determined. The greatest influence of quercetin and histamine on erythrocytes state indicators was revealed under their combined action, when the level of TBA-active products and the content of carbonyl groups of proteins were found to be increased substantially

Glutation-S-transferase activity of loach embryos during embryogenesis under the action of sodium hypochlorite

he effect of sodium hypochlorite obtained at electrolysis of isotonic solution of sodium chloride that is a source of active oxygen on activity of glutation-s-transferase in loach embryos was studied. It was shown that sodium hypochlorite in concentrations 0,5, 1, 2,5, 5, 7,5, 10, 12,5 µg/l caused an increase of glutation-s-transferase activity on the stages of development loach embryos 2, 16, 64, 256, 1024 blastodmeres. On the stage of embryonic development at 64 blastomeres, that corresponds to the sixth dividing of zygote the activity of glutation-s-transferase is maximal and rises, comparative to control, approximatevely on 136% at the action of sodium hypochlorite in all investigated concentrations. While in control samples, the activity of this enzyme is the lowest, comparatively with the stages of development of 2, 16, 256 and 1024 blastomeres. Probably, on this stage of division of embryos, the sodium hypochlorite causes a formation of harmful compounds (for example organic peroxides), that can be inactivated by the glutation-s-transferase. It is found that during incubation of bioblasts in the medium with sodium hypochlorite on the stage of 10th division, the enzymatic activity increased in a dose–dependent maner. Two-factor analysis of variance show that glutation-s-transferase activity depends on the time of development of loach embryos and sodium hypochlorite

Effect of sodium hypochlorite on prooxidant and antioxidant homeostasis of loach embryos during early embryogenesis

The article contains data regarding the influence of sodium hypochlorite on the processes of free radical oxidation and antioxidant system of loach Misgurnus fossilis L. embryos in early stages of the development. It was showed that the effect of sodium hypochlorite added to the medium where the embryos develop, leads to disruption of prooxidation-antioxidant loach Misgurnus fossilis L. embryos homeostasis during early embryogenesis. It is proved that the sodium hypochlorite solution is empowered prooxydant manifestations in the early stages of embryogenesis wich are investigated and is inhibiting certain enzymes of antioxidant protection. It was found that the embryos at the 16 blastomeres stage of early embryogenesis are most sensitive to the action of sodium hypochlorite. Embryos at the 1024 blastomeres stage (10 division) are the most resistant to sodium hypochlorite as the content of lipid peroxidation products is reduced compared with control, whereas the activity of superoxidedismutase and glutathione peroxidase is within control value

Development of obtaining stable substances for functional food products based on yacon (Smallanthus sonchifolius Poepp. & Endl.) powder root tubers and investigation of their hypoglycemic activity in rats

Methods of obtaining a stable substance for functional foods with antioxidant and hypoglycemic action based on powder root tubers of yacon’ are proposed. The aim of the study was to develop a substance stabilizing method which can be the basis for functional food, using yacons root tubers and investigate the changes of hypoglycemic effect of yacons’ root tubers water suspensions after stabilization with PS biocomplex under experimental model of diabetes. Stabilized basis for functional food product were received in several ways: by adding to control suspension a surface-active PS biocomplex in concentrations of 0.00001 g/ml, 0.00002 g/ml, 0.00003 g/ml per 1 ml suspension and by using yacons root tubers powder that were pre-mixed with PS biocomplex in the concentration of 0.00001 g/ml of water. It was shown that both methods of obtaining stable substances for functional foods ensure getting the aggregation stable suspensions. Application method of PS biocomplex do not particularly affect a stability of the studied substances, but preliminary addition of PS biocomplex to the powder is convenient for development of biological preparations encapsulated forms manufacturing technology. Addition of PS biocomplex to the suspension of yacons’ root tubers allows increasing the accuracy of substance dosing and increasing bioavailability of bioactive substances, that was indicated by more severe hypoglycemic effect of a suspension stabilized form

Nа+, K+-ATPase activity of loach embryos membranes during early embryogenesis under the influence of sodium hypochlorite

The paper contains data regarding the influence of sodium hypochlorite on the activity of embryos membrane Na+, K+-ATP-ase of loach Misgurnus fossilis L. in early stages of the development. We showed that sodium hypochlorite added to the medium where the embryos develop, caused dose-dependent inhibition of the membranes Na+, K+-ATP-ase activity. We suggested that effects of sodium hypochlorite were due to oxidizing properties and its ability to disrupt membrane integrity, in particular, damaging proteins and lipids