ЭФФЕКТ НИТРОГЛИЦЕРИНА НА ПРОЛИФЕРАТИВНЫЙ И МИГРАЦИОННЫЙ ПОТЕНЦИАЛ МОНОНУКЛЕАРНЫХ КЛЕТОК ПЕРИФЕРИЧЕСКОЙ КРОВИ

Aim. To study the effects of nitroglycerin on proliferative and migratory activity of mononuclear cells and endothelial progenitor cells in patients with chronic heart failure.Materials and methods. Proliferative potential of peripheral blood mononuclear cells in donors and patients were studied in MTT-test in the presence of 50 and 100 mg/ml nitroglycerin. Proliferation and migration of endothelial progenitor cells in patients were evaluated by changes in the cell impedance in real-time in the presence of various doses of nitroglycerin.Results. It is shown that nitroglycerin reduces in vitro both spontaneous and Concanavalin A stimulated proliferative activity of mononuclear cells in patients with chronic heart failure. Peripheral blood mononuclear cells in patients with chronic heart failure, obtained after procedure of mobilization by granulocyte colony-stimulating factor respond to nitroglycerin dose of 50 mg/ml with the increased proliferative activity, and with the suppressed proliferative activity to nitroglycerin dose of 100 mg/ml. Mononuclear cells of healthy donors respond to 50 mg/ml of nitroglycerin with the activation of proliferative capacity, but at a dose of 100 mg/ml spontaneous proliferative activity of mononuclear cells inhibit. It is also shown that the presence of nitroglycerin in medium dose in stimulating test leads to the inhibition of mononuclear cells proliferation from healthy donors. It is shown the stimulating effect of nitroglycerin on proliferative and migration potential of endothelial progenitor cells.Conclusion. Multidirectional and dose-dependent effects of nitroglycerin to the various types of human cells in vitro were observed.Цель. Изучить эффекты влияния нитроглицерина на пролиферативную и миграционную активность мононуклеарных клеток и эндотелиальных прогениторных клеток у пациентов с хронической сердечнойнедостаточностью.Материалы и методы. Пролиферативный потенциал мононуклеарных клеток периферической крови у доноров и пациентов изучали в МТТ-тесте в присутствии 50 и 100 мкг/мл нитроглицерина, пролиферацию и миграцию эндотелиальных прогениторных клеток пациентов оценивали по изменению клеточного импеданса в режиме реального времени в присутствии различных доз нитроглицерина.Результаты. Показано, что нитроглицерин снижает пролиферативную активность in vitro мононуклеарных клеток пациентов с хронической сердечной недостаточностью, как спонтанную, так и стимулированную Конканавалин А. Мононуклеарные клетки периферической крови пациентов с хронической сердечной недостаточностью, полученные после процедуры мобилизации гранулоцитарным колониестимулирующим фактором, отвечают увеличением пролиферативной активности на нитроглицерин в дозе 50 мкг/мл, а при дозе 100 мкг/мл – подавлением пролиферативной активности. Мононуклеары условно здоровых доноров в ответ на стимуляцию нитроглицерином отвечают активацией пролиферативного потенциала. Показан стимулирующий эффект нитроглицерина на пролиферативный и миграционный потенциал эндотелиальных прогениторных клеток.Заключение. Установлен разнонаправленный и дозозависимый эффект нитроглицерина на различные типы клеток человека in vitro

THE EFFECTS OF NITROGLYCERIN ON THE PROLIFERATIVE AND MIGRATORY POTENTIAL OF PERIPHERAL BLOOD MONONUCLEAR CELLS

Aim. To study the effects of nitroglycerin on proliferative and migratory activity of mononuclear cells and endothelial progenitor cells in patients with chronic heart failure.Materials and methods. Proliferative potential of peripheral blood mononuclear cells in donors and patients were studied in MTT-test in the presence of 50 and 100 mg/ml nitroglycerin. Proliferation and migration of endothelial progenitor cells in patients were evaluated by changes in the cell impedance in real-time in the presence of various doses of nitroglycerin.Results. It is shown that nitroglycerin reduces in vitro both spontaneous and Concanavalin A stimulated proliferative activity of mononuclear cells in patients with chronic heart failure. Peripheral blood mononuclear cells in patients with chronic heart failure, obtained after procedure of mobilization by granulocyte colony-stimulating factor respond to nitroglycerin dose of 50 mg/ml with the increased proliferative activity, and with the suppressed proliferative activity to nitroglycerin dose of 100 mg/ml. Mononuclear cells of healthy donors respond to 50 mg/ml of nitroglycerin with the activation of proliferative capacity, but at a dose of 100 mg/ml spontaneous proliferative activity of mononuclear cells inhibit. It is also shown that the presence of nitroglycerin in medium dose in stimulating test leads to the inhibition of mononuclear cells proliferation from healthy donors. It is shown the stimulating effect of nitroglycerin on proliferative and migration potential of endothelial progenitor cells.Conclusion. Multidirectional and dose-dependent effects of nitroglycerin to the various types of human cells in vitro were observed