3 research outputs found
ΠΠ€Π€ΠΠΠ’ ΠΠΠ’Π ΠΠΠΠΠ¦ΠΠ ΠΠΠ ΠΠ ΠΠ ΠΠΠΠ€ΠΠ ΠΠ’ΠΠΠΠ«Π Π ΠΠΠΠ ΠΠ¦ΠΠΠΠΠ«Π ΠΠΠ’ΠΠΠ¦ΠΠΠ ΠΠΠΠΠΠ£ΠΠΠΠΠ ΠΠ«Π₯ ΠΠΠΠ’ΠΠ ΠΠΠ ΠΠ€ΠΠ ΠΠ§ΠΠ‘ΠΠΠ ΠΠ ΠΠΠ
Aim. To study the effects of nitroglycerin on proliferative and migratory activity of mononuclear cells and endothelialΒ progenitor cells in patients with chronic heart failure.Materials and methods. Proliferative potentialΒ of peripheral blood mononuclear cells in donors and patients were studied in MTT-test in the presence of 50 andΒ 100 mg/ml nitroglycerin. Proliferation and migration of endothelial progenitor cells in patients were evaluated by changes in the cell impedance in real-time in the presence of various doses of nitroglycerin.Results. It is shown that nitroglycerin reduces in vitro both spontaneous and Concanavalin A stimulated proliferative activity of mononuclear cells in patients with chronic heart failure. Peripheral blood mononuclear cells in patients with chronic heart failure, obtained after procedure of mobilization by granulocyte colony-stimulating factor respond to nitroglycerin dose of 50 mg/ml with the increased proliferative activity, and with the suppressed proliferative activity to nitroglycerin dose of 100 mg/ml. Mononuclear cells of healthy donors respond to 50 mg/ml of nitroglycerin with the activation of proliferative capacity, but at a dose of 100 mg/ml spontaneous proliferative activity of mononuclear cells inhibit. It is also shown that the presence of nitroglycerin in medium dose in stimulating test leads to the inhibition of mononuclear cells proliferation from healthy donors. It is shown the stimulating effect of nitroglycerin on proliferative and migration potential of endothelial progenitor cells.Conclusion.Β Multidirectional and dose-dependent effects of nitroglycerin to the various types of human cells in vitro were observed.Π¦Π΅Π»Ρ. ΠΠ·ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΡ Π²Π»ΠΈΡΠ½ΠΈΡ Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½Π° Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ ΠΈ ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΌΠΎΠ½ΠΎΠ½ΡΠΊΠ»Π΅Π°ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΠ³Π΅Π½ΠΈΡΠΎΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎΠΉΠ½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΡΡ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» ΠΌΠΎΠ½ΠΎΠ½ΡΠΊΠ»Π΅Π°ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Ρ Π΄ΠΎΠ½ΠΎΡΠΎΠ² ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΈΠ·ΡΡΠ°Π»ΠΈ Π² ΠΠ’Π’-ΡΠ΅ΡΡΠ΅ Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ 50 ΠΈ 100 ΠΌΠΊΠ³/ΠΌΠ» Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½Π°, ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ ΠΈ ΠΌΠΈΠ³ΡΠ°ΡΠΈΡ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΠ³Π΅Π½ΠΈΡΠΎΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΒ ΠΏΠΎ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΠΈΠΌΠΏΠ΅Π΄Π°Π½ΡΠ° Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π΄ΠΎΠ· Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½Π°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½ ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΡ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ in vitroΒ ΠΌΠΎΠ½ΠΎΠ½ΡΠΊΠ»Π΅Π°ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎΠΉ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΡΡ, ΠΊΠ°ΠΊ ΡΠΏΠΎΠ½ΡΠ°Π½Π½ΡΡ, ΡΠ°ΠΊ ΠΈΒ ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΠΎΠ½ΠΊΠ°Π½Π°Π²Π°Π»ΠΈΠ½ Π. ΠΠΎΠ½ΠΎΠ½ΡΠΊΠ»Π΅Π°ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ΄Π΅ΡΠ½ΠΎΠΉ Π½Π΅Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎΡΡΡΡ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΠΏΠΎΡΠ»Π΅ ΠΏΡΠΎΡΠ΅Π΄ΡΡΡ ΠΌΠΎΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΈΠΈ Π³ΡΠ°Π½ΡΠ»ΠΎΡΠΈΡΠ°ΡΠ½ΡΠΌΒ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ΅ΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠΈΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ, ΠΎΡΠ²Π΅ΡΠ°ΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π° Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½ Π² Π΄ΠΎΠ·Π΅ 50 ΠΌΠΊΠ³/ΠΌΠ», Π° ΠΏΡΠΈ Π΄ΠΎΠ·Π΅ 100 ΠΌΠΊΠ³/ΠΌΠ» β ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ. ΠΠΎΠ½ΠΎΠ½ΡΠΊΠ»Π΅Π°ΡΡΒ ΡΡΠ»ΠΎΠ²Π½ΠΎ Π·Π΄ΠΎΡΠΎΠ²ΡΡ
Π΄ΠΎΠ½ΠΎΡΠΎΠ² Π² ΠΎΡΠ²Π΅Ρ Π½Π° ΡΡΠΈΠΌΡΠ»ΡΡΠΈΡ Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½ΠΎΠΌ ΠΎΡΠ²Π΅ΡΠ°ΡΡ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠ΅ΠΉ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»Π°. ΠΠΎΠΊΠ°Π·Π°Π½ ΡΡΠΈΠΌΡΠ»ΠΈΡΡΡΡΠΈΠΉ ΡΡΡΠ΅ΠΊΡ Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½Π° Π½Π° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΡΠΉ ΠΈ ΠΌΠΈΠ³ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π» ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΏΡΠΎΠ³Π΅Π½ΠΈΡΠΎΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ ΡΠ°Π·Π½ΠΎΠ½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉΒ ΠΈ Π΄ΠΎΠ·ΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΡΡΡΠ΅ΠΊΡ Π½ΠΈΡΡΠΎΠ³Π»ΠΈΡΠ΅ΡΠΈΠ½Π° Π½Π° ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ ΡΠΈΠΏΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° in vitro
THE EFFECTS OF NITROGLYCERIN ON THE PROLIFERATIVE AND MIGRATORY POTENTIAL OF PERIPHERAL BLOOD MONONUCLEAR CELLS
Aim. To study the effects of nitroglycerin on proliferative and migratory activity of mononuclear cells and endothelialΒ progenitor cells in patients with chronic heart failure.Materials and methods. Proliferative potentialΒ of peripheral blood mononuclear cells in donors and patients were studied in MTT-test in the presence of 50 andΒ 100 mg/ml nitroglycerin. Proliferation and migration of endothelial progenitor cells in patients were evaluated by changes in the cell impedance in real-time in the presence of various doses of nitroglycerin.Results. It is shown that nitroglycerin reduces in vitro both spontaneous and Concanavalin A stimulated proliferative activity of mononuclear cells in patients with chronic heart failure. Peripheral blood mononuclear cells in patients with chronic heart failure, obtained after procedure of mobilization by granulocyte colony-stimulating factor respond to nitroglycerin dose of 50 mg/ml with the increased proliferative activity, and with the suppressed proliferative activity to nitroglycerin dose of 100 mg/ml. Mononuclear cells of healthy donors respond to 50 mg/ml of nitroglycerin with the activation of proliferative capacity, but at a dose of 100 mg/ml spontaneous proliferative activity of mononuclear cells inhibit. It is also shown that the presence of nitroglycerin in medium dose in stimulating test leads to the inhibition of mononuclear cells proliferation from healthy donors. It is shown the stimulating effect of nitroglycerin on proliferative and migration potential of endothelial progenitor cells.Conclusion.Β Multidirectional and dose-dependent effects of nitroglycerin to the various types of human cells in vitro were observed