New insights into the synergism of nucleoside analogs with radiotherapy

Nucleoside analogs have been frequently used in combination with radiotherapy in the clinical setting, as it has long been understood that inhibition of DNA repair pathways is an important means by which many nucleoside analogs synergize. Recent advances in our understanding of the structure and function of deoxycytidine kinase (dCK), a critical enzyme required for the anti-tumor activity for many nucleoside analogs, have clarified the mechanistic role this kinase plays in chemo- and radio-sensitization. A heretofore unrecognized role of dCK in the DNA damage response and cell cycle machinery has helped explain the synergistic effect of these agents with radiotherapy. Since most currently employed nucleoside analogs are primarily activated by dCK, these findings lend fresh impetus to efforts focused on profiling and modulating dCK expression and activity in tumors. In this review we will briefly review the pharmacology and biochemistry of the major nucleoside analogs in clinical use that are activated by dCK. This will be followed by discussions of recent advances in our understanding of dCK activation via post-translational modifications in response to radiation and current strategies aimed at enhancing this activity in cancer cells

THE INFLUENCE OF VHF ELECTROMAGNETIC RADIATION ON THE FREQUENCIES OF MOLECULAR SPECTRUM AND OXYGEN ABSORPTION ON BLOOD REOLOGICAL PROPERTIES IN STABLE ANGINA

The results of studing main features of influence of electromagnetic radiation of atmosphere oxygen molecular spectra of irradiation and absorption frequencies on whole blood rheology of patients with stable angina pectoris were present. The changes of rheological properties of whole blood samples of the patients with stable angina pectoris irradiated with electromagnetic radiation of atmosphere oxygen molecular spectra of irradiation and absorption frequencies depend on the time of treating, initial haematocrit level, fibrinogen concentration and arterial blood pressure

Platelets of unstable angina patients as effector cells in terahertz radiation at NO frequency

Aim. To investigate the effects of electromagnetic terahertz waves at NO MSIP (molecular specter of radiation and absorption), 240 Hz, on platelet functional activity Р in natural electromagnetic field, as well as in crossed magnetic and electric fields among unstable angina patients. Material and methods. This in vitro study was performed on samples of platelet-enriched plasma from 120 unstable angina patients. The study of terahertz waves and platelets interaction at NO MSRA specter, 240 GHz, was performed with original quasi-optic SW generator of determined noises (CRIMD, Saratov). Functional activity of platelets (activation and aggregation) was measured by laser aggregation analyzer (Biola-230 Ltd.). Results. After in-vitro radiation of platelet-enriched plasma from unstable angina patients, at NO MSRA frequency, 240 GHz, disturbed platelet aggregation activity was normalized. Conclusion. Therefore, terahertz waves, at NO frequency, 240 GHz, substantially decreased elevated aggregation activity of the platelets from unstable angina patients