MOLECULAR DIAGNOSTICS IN BIOLOGY AND MEDICINE

W ostatnim dziesięcioleciu rozwój i wdrożenie technik molekularnych zrewolucjonizowało diagnostykę i monitorowanie chorób człowieka. Techniki badań kwasów nukleinowych, takie jak genotypowanie DNA mikroorganizmów, analiza polimorfizmu fragmentów restrykcyjnych (RFLP) i reakcja łańcuchowa polimerazy (PCR) przyczyniają się do stałego postępu w laboratoriach diagnostyki medycznej. Analiza sekwencji amplifikowanych fragmentów DNA umożliwia lepszą identyfikację patogenów, wykrywanie zmutowanych genów a także prognozowanie określonych chorób.In the last decade, the development and application of molecular techniques has revolutionised the diagnosis and monitoring of human diseases. Nucleic acids techniques, such as microbial DNA genotyping, analysis of restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR), are making progress in medical diagnostic laboratories. Sequence analysis of amplified DNA allows better identification of pathogen, detection of mutant genes and more accurate prognosis of certain diseases

A radiographic facility at the Soltan Institute for Nuclear Studies (SINS) at Świerk, Poland

This paper describes the 6 MeV linac electron accelerator facility now operating at SINS, Świerk, Poland. The accelerator can work in both the electron or X-ray photon mode. The photon beam may be used for non-destructive radiographic investigations on a laboratory scale and, with some modifications, for industrial purposes, as well. As the accelerator's removable tungsten e/X converter is placed outside, the beam vacuum window, an external electron beam is also accessible. The design stages of the accelerator's construction are described, and examples of measurements and radiographic pictures are also presented

The IOERT IntraLine accelerator : the development, current state, and future plans

Intraoperative electron radiation therapy (IOERT) is a cancer treatment method that combines oncologic surgery with electron beam radiotherapy. This method can reduce the duration of entire tumor treatment and increase its effectiveness. Moreover, shortening the treatment time signifi cantly reduces the cost and accessibility of the therapy. As a result of interdisciplinary research, an innovative accelerator for IOERT, the IntraLine, was developed. In the course of this work, four patent applications were fi led. Today, the work is half way through. In the near future, the device will be optimized. New mechanical design solutions will be developed. Mechanical optimization will signifi cantly reduce the weight of the device. Accelerators control system, which today is in the demo phase, will also be signifi cantly upgraded. This paper describes the stages of the IntraLine accelerator development, its current state and plans for the future R&D work, within the scope of our new Intra-Dose project