Spatially resolved generation profiles for building, land and water-bound PV: a case study of four Dutch energy transition scenarios

Alongside a transition from steerable and centralized traditional electricity generation to intermittent and more decentralized renewable electricity generation from solar panels and wind turbines, Dutch energy transition scenarios project a widespread deployment of heat pumps and electric vehicles towards 2050. While clearly contributing to the decarbonization of the Dutch energy system, these developments impose challenges regarding electricity supply-demand mismatch and grid congestion. Spatially resolved electricity demand and supply profiles are required to gain a better insight into where and when such problems are likely to occur within the different scenarios. The present paper focuses on Dutch solar energy supply and features the construction of geodatabases of scenario-specific, spatially resolved electricity generation profiles for building, land and water-bound PV. Country-level PV capacities are geographically distributed based on spatial variance in roof PV potential and availability of suitable land and water use areas. Corresponding electricity generation profiles are constructed using historical meteorological measurements, a diffuse fraction model and a anisotropic transposition model. Empirically found performance ratio profiles are applied to account for a multitude of performance loss factors, including shading, dust and inverter efficiency. In 2050, building-bound capacity is projected to show only limited overlap with both land-bound and water-bound PV capacity. On the other hand, regions with considerable water-bound PV capacity also tend to show considerable land-bound PV capacity. Compared to the present-day situation, yearly country-level PV electricity generation is projected to be a factor 18.5, 15.7, or 7.7 higher in 2050 when respectively following the Regional, National or International Steering scenarios.</p

Production of Native Bispecific Antibodies in Rabbits

BACKGROUND: A natural bispecific antibody, which can be produced by exchanging Fab arms of two IgG4 molecules, was first described in allergic patients receiving therapeutic injections with two distinct allergens. However, no information has been published on the production of natural bispecific antibody in animals. Even more important, establishment of an animal model is a useful approach to investigate and characterize the naturally occurring antibody. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrated that a natural bispecific antibody can also be generated in New Zealand white rabbits by immunization with synthesized conjugates. These antibodies showed bispecificity to the components that were simultaneously used to immunize the animals. We observed a trend in our test animals that female rabbits exhibited stronger bispecific antibody responses than males. The bispecific antibody was monomeric and primarily belonged to immunoglobulin (Ig) G. Moreover, bispecific antibodies were demonstrated by mixing 2 purified monospecific antibodies in vivo and in vitro. CONCLUSIONS/SIGNIFICANCE: Our results extend the context of natural bispecific antibodies on the basis of bispecific IgG4, and may provide insights into the exploration of native bispecific antibodies in immunological diseases

Sonication is superior to scraping for retrieval of bacteria in biofilm on titanium and steel surfaces in vitro

Background and purpose Low-virulence implant infections are characterized by bacterial colonization of the implant with subsequent biofilm formation. In these cases, soft tissue biopsies often prove to be culture negative. Consequently, detachment of the causative adherent bacteria is crucial for correct microbiological diagnosis. Using an in vitro model, we compared 4 methods of biofilm sampling from metal surfaces

Sulindac targets nuclear β-catenin accumulation and Wnt signalling in adenomas of patients with familial adenomatous polyposis and in human colorectal cancer cell lines

Nonsteroidal anti-inflammatory drugs (NSAIDs) have chemopreventive potential against colorectal carcinomas (CRCs). Inhibition of cyclooxygenase (COX)-2 underlies part of this effect, although COX-2-independent mechanisms may also exist. Nonsteroidal anti-inflammatory drugs appear to inhibit the initial stages of the adenoma-carcinoma sequence, suggesting a link to the APC/beta-catenin/TCF pathway (Wnt-signalling pathway). Therefore, the effect of the NSAID sulindac on nuclear (nonphosphorylated) beta-catenin and beta-catenin/TCF-mediated transcription was investigated. Nuclear #946;-catenin expression was assessed in pretreatment colorectal adenomas and in adenomas after treatment with sulindac from five patients with familial adenomatous polyposis (FAP). Also, the effect of sulindac sulphide on beta-catenin/TCF-mediated transcription was studied. Adenomas of FAP patients collected after treatment with sulindac for up to 6 months showed less nuclear beta-catenin expression compared to pretreatment adenomas of the same patients. Sulindac sulphide abrogated beta-catenin/TCF-mediated transcription in the CRC cell lines DLD1 and SW480, and decreased the levels of nonphosphorylated beta-catenin. As a result, the protein levels of the positively regulated TCF targets Met and cyclin D1 were downregulated after sulindac treatment. This study provides in vivo and in vitro evidence that nuclear beta-catenin localisation and beta-catenin/TCF-regulated transcription of target genes can be inhibited by sulindac. The inhibition of Wnt-signalling provides an explanation for the COX-2-independent mechanism of chemoprevention by NSAID