33 research outputs found
Cucumber mosaic virus as a possible carrier of an epitope deriving from hepatitis C virus
ADDENDUM II, 52-
Structural and biological properties of cucumber mosaic virus particles carrying hepatitis c virus-derived epitopes.
The Cucumber mosaic virus (CMV) is a three-component isodiametric plant virus with an extremely wide
host range, present worldwide. A pseudorecombinant form has been described, deriving from the RNA3
component of the CMV-S strain, carrying the coat protein (CP) gene, and the RNA 1, 2 components of the
CMV-D strain. The CP gene was then engineered to express one or two copies of a synthetic peptide derived
from many hypervariable region 1 (HVR1) sequences of the Hepatitis C virus (HCV) envelope protein E2
(the so-called R9 mimotope).
Study of the symptoms pattern displayed in tobacco by these chimeric CMV particles, together with
determination of their structural characteristics, assessed by circular dichroism (CD) spectroscopy and
electron microscopy, revealed a possible relationship between the biological behavior and the structural
properties of virus components
Structural studies of chimeric cucumber mosaic virus (CMV) particles carrying a hepatitis C virus-derived epitope.
ADDENDUM II, 52-
Cucumber mosaic virus as carrier of a hepatitis C virus-derived epitope.
Cucumber mosaic virus (CMV) is a three component isodiametric
plant virus which is common worldwide and has an extremely wide host range.A
pseudorecombinant was made, derived from the RNA3 component of the CMVS
strain, carrying the coat protein (CP) gene, and the RNA1,2 components of
the CMV-D strain. This system developed mild mosaic and vein clearing in
Xanthi tobacco three weeks after inoculation. The CP gene was then engineered
in three different positions, to encode a Hepatitis C virus (HCV) epitope. The
selected peptide was the so-called R9 mimotope, a synthetic surrogate derived
from a consensus profile of many hypervariable region 1 (HVR1) sequences of
the putative HCV envelope protein E2. Serum samples from 60 patients with
chronic hepatitisCdisplayed a significant immunoreactivity to crude plant extracts
infected with the chimeric CMV. These results suggest that further investigation
should be made into a possible vaccine function for the CMV-HCV mimotope
system
Structural and evolutionary analysis of the ribosomal genes of the parasitic nematode Meloidogyne artiellia suggests its ancient origin
Cucumber mosaic virus as carrier of a hepatitis C virus-derived epitope
Cucumber mosaic virus (CMV) is a three component isodiametric
plant virus which is common worldwide and has an extremely wide host range.A
pseudorecombinant was made, derived from the RNA3 component of the CMVS
strain, carrying the coat protein (CP) gene, and the RNA1,2 components of
the CMV-D strain. This system developed mild mosaic and vein clearing in
Xanthi tobacco three weeks after inoculation. The CP gene was then engineered
in three different positions, to encode a Hepatitis C virus (HCV) epitope. The
selected peptide was the so-called R9 mimotope, a synthetic surrogate derived
from a consensus profile of many hypervariable region 1 (HVR1) sequences of
the putative HCV envelope protein E2. Serum samples from 60 patients with
chronic hepatitisCdisplayed a significant immunoreactivity to crude plant extracts
infected with the chimeric CMV. These results suggest that further investigation
should be made into a possible vaccine function for the CMV-HCV mimotope
system