Phosphatidase A Activity of Ammodytes Viper Venom

The venom of Vipera Ammodytes was resolved by electrophoresis in seven proteinic components. These components were tested for phosphatidase A activity. The reliability of the method for haemolysis activity determination is discussed

Phosphatidase A Activity of Ammodytes Viper Venom

The venom of Vipera Ammodytes was resolved by electrophoresis in seven proteinic components. These components were tested for phosphatidase A activity. The reliability of the method for haemolysis activity determination is discussed

Kromatografija insulina na papiru

Paper strip chromatography of commercial samples of insulin using Whatman No. I. filter paper and ten serial dilutions of 3 M sodium chloride in veronal buffer pH 8.6 as eluent has been presented.Prikazana je kromatografija na papiru kupovnih uzoraka kristaliničnog inzulina, upotrebom filtar-papira Whatman br. l. i razvijanjem kromatograma nizom otopina natrijeva klorida u rastućem gradijentu koncentracija (0,003 - 3 M) u veronalskom puferu pH 8,6

The use of radioactive chromium (Cr51) in the determination of red cell survival

Proučavanje dužine života eritrocita pojednostavnjeno je upotrebom radioaktivnog kroma, a njegovom primjenom u mogućnosti smo pratiti ponašanje bolesnikovih eritrocita nakon autotransfuzije, kao i ponašanje eritrocita davaoca u bolesniku. Opisane su metode tih istraživanja i raspravljena su tumačenja za različne tipove krivulja. Prikazani su primjeri krivulja dužine života eritrocita kod različitih oblika hemolitskih anemija. Eritrociti, koji su označeni radioaktivnim kromom, mogu se također upotrebiti za određivanje mjesta raspada eritrocita in vivo, a taj test može biti od naročite koristi za splenektomiju.By usage of radioactive chromium, the labelling of erythrocytes for the study of cell survival has been simplified and it is possible to study the behaviour of a patient\u27s own cells after auto transfusion as well as the behaviour of donor cells in the patient. The methods for these investigations are described and the interpretation of different types of curves are discussed. Examples are given of the survival pattern in various forms of haemolytic anaemia. Chromium labelled-erythrocytes can also be used for the estimation of the assessment of sites of destruction of erythrocytes in vivo, This latter test is of especial use in determining the value of splenectomy in controlling haemolysis

Kromatografija proteina na papiru

The chromatographic techniques using paper fibers as adsorbent and several step elution of proteins with salt solutions of increasing concentrations have been developed. The influence of electrolytes on the chromatographic resolution of proteins has been presented. The chromatographic separation of a series of natural and artificial mixtures has been set forth. The chambers for ascending and descending paperstrip chromatography procedures with salt concentration gradient have been described.Opisani su postupci uzlazne i izlazne kromatografije proteina, kod kojih se upotrebljava papir kao adsorbens, a za eluciju niz otopina elektrolita u rastućim koncentracijama. Ispitan je utjecaj elektrolita na kromatografsko razdvajanje proteinskih smjesa. Prikazane su originalne aparature za uzlaznu i silaznu novopredloženu kromatografsku tehnik

Kromatografija insulina na papiru

Paper strip chromatography of commercial samples of insulin using Whatman No. I. filter paper and ten serial dilutions of 3 M sodium chloride in veronal buffer pH 8.6 as eluent has been presented.Prikazana je kromatografija na papiru kupovnih uzoraka kristaliničnog inzulina, upotrebom filtar-papira Whatman br. l. i razvijanjem kromatograma nizom otopina natrijeva klorida u rastućem gradijentu koncentracija (0,003 - 3 M) u veronalskom puferu pH 8,6