Agile chip-scale electro-optic frequency comb spectrometer with millivolt drive voltages

Here, we present an on-chip spectrometer that leverages an integrated thin-film lithium niobate modulator to produce a frequency-agile electro-optic frequency comb for interrogating chip-scale temperature and acceleration sensors. The low half-wave voltage, $V_{\pi}$, of the modulators and the chirped comb process allows for ultralow radiofrequency drive voltages, which are as much as seven orders of magnitude less than the lowest found in the literature and are generated using a chip-scale, microcontroller-driven direct digital synthesizer. The on-chip comb spectrometer is able to simultaneously interrogate both the on-chip temperature sensor and an off-chip, microfabricated optomechanical accelerometer with cutting-edge sensitivities of $\approx 5 {\mu} \mathrm{K} \cdot \mathrm{Hz} ^{-1/2}$ and $\approx 130 {\mu}\mathrm{m} \cdot \mathrm{s}^{-2} \cdot \mathrm{Hz}^{-1/2}$, respectively. Notable strengths of this platform include the frequency agility of the optical frequency combs, ultralow radiofrequency power requirements and compatibility with a broad range of existing photonic integrated circuit technologies.Comment: 11 pages, 5 figure

The Nanolithography Toolbox

This article introduces in archival form the Nanolithography Toolbox, a platform-independent software package for scripted lithography pattern layout generation. The Center for Nanoscale Science and Technology (CNST) at the National Institute of Standards and Technology (NIST) developed the Nanolithography Toolbox to help users of the CNST NanoFab design devices with complex curves and aggressive critical dimensions. Using parameterized shapes as building blocks, the Nanolithography Toolbox allows users to rapidly design and layout nanoscale devices of arbitrary complexity through scripting and programming. The Toolbox offers many parameterized shapes, including structure libraries for micro- and nanoelectromechanical systems (MEMS and NEMS) and nanophotonic devices. Furthermore, the Toolbox allows users to precisely define the number of vertices for each shape or create vectorized shapes using Bezier curves. Parameterized control allows users to design smooth curves with complex shapes. The Toolbox is applicable to a broad range of design tasks in the fabrication of microscale and nanoscale devices

Genomic analysis of Acinetobacter baumannii prophages reveals remarkable diversity and suggests significant impact on bacterial virulence and fitness

[Abstract] Bacterial genomics has revealed substantial amounts of prophage DNA in bacterial genomes. This integrated viral DNA has been shown to play important roles in the evolution of bacterial pathogenicity. Acinetobacter baumannii has shown a fast progression as a nosocomial multi-resistant pathogen in recent years, and is now considered one of the most dangerous microorganisms in hospital environments. The role of prophages in the evolution of A. baumannii pathogenicity has not yet been explored. In this context, we aimed at evaluating the impact of prophages on A. baumannii genomic diversity and pathogenicity. [...]info:eu-repo/semantics/publishedVersio

Pulse shaping of on-chip microresonator frequency combs: investigation of temporal coherence

We use pulse shaping to investigate the temporal coherence of frequency combs generated in microresonators pumped by a strong CW laser. We observe that different groups of comb lines have different mutual coherence

Development and validation of a multiplex immunoassay for the simultaneous quantification of type-specific IgG antibodies to E6/E7 oncoproteins of HPV16 and HPV18.

More than 170 types of human papilloma viruses (HPV) exist with many causing proliferative diseases linked to malignancy in indications such as cervical cancer and head and neck squamous cell carcinoma. Characterization of antibody levels toward HPV serology is challenging due to complex biology of oncoproteins, pre-existing titers to multiple HPV types, cross-reactivity, and low affinity, polyclonal responses. Using multiplex technology from MSD, we have developed an assay that simultaneously characterizes antibodies against E6 and E7 oncoproteins of HPV16 and 18, the primary drivers of HPV-associated oncogenesis. We fusion tagged our E6 and E7 proteins with MBP via two-step purification, spot-printed an optimized concentration of protein into wells of MSD 96-well plates, and assayed various cynomolgus monkey, human and HPV+ cervical cancer patient serum to validate the assay. The dynamic range of the assay covered 4-orders of magnitude and antibodies were detected in serum at a dilution up to 100,000-fold. The assay was very precise (n = 5 assay runs) with median CV of human serum samples ~ 5.3% and inter-run variability of 11.4%. The multiplex serology method has strong cross-reactivity between E6 oncoproteins from human serum samples as HPV18 E6 antigens neutralized 5 of 6 serum samples as strongly as HPV16 E6. Moderate concordance (Spearman's Rank = 0.775) was found between antibody responses against HPV16 E7 in the multiplex assay compared to standard ELISA serology methods. These results demonstrate the development of a high-throughput, multi-plex assay that requires lower sample quantity input with greater dynamic range to detect type-specific anti-HPV concentrations to E6 and E7 oncoproteins of HPV16 and 18