Two-photon fluorescence isotropic-single-objective microscopy

International audienceTwo-photon excitation provides efficient optical sectioning in three-dimensional fluorescence microscopy, independently of a confocal detection. In two-photon laser-scanning microscopy, the image resolution is governed by the volume of the excitation light spot, which is obtained by focusing the incident laser beam through the objective lens of the microscope. The light spot being strongly elongated along the optical axis, the axial resolution is much lower than the transverse one. In this Letter we show that it is possible to strongly reduce the axial size of the excitation spot by shaping the incident beam and using a mirror in place of a standard glass slide to support the sample. Provided that the contribution of sidelobes can be removed through deconvolution procedures, this approach should allow us to achieve similar axial and lateral resolution

Isotropic Single Objective (ISO) microscopy : Theory and Experiment

International audienceIsotropic single-objective (ISO) microscopy is a recently proposed imaging technique that can theoretically exhibit the same axial and transverse resolutions as 4Pi microscopy while using a classical single-objective confocal microscope. This achievement is obtained by placing the sample on a mirror and shaping the illumination beam so that the interference of the incident and mirror-reflected fields yields a quasi-spherical spot. In this work, we model the image formation in the ISO fluorescence microscope and simulate its point spread function. Then, we describe the experimental implementation and discuss its practical difficulties

Construindo a identidade agroecológica através de trocas de experiências entre assentamentos da reforma agrária no estado de São Paulo.

Resumo: Este trabalho faz parte do projeto ?Capacitação sócio-ambiental para construção de projetos de desenvolvimento sustentável em assentamentos rurais no estado de São Paulo?, uma parceria da Embrapa Meio Ambiente com o INCRA, MST e outras organizações de agricultores assentados. Assim, propomos apresentar uma etapa dos trabalhos que vem sendo desenvolvido nos assentamentos Itapeva e Sepé Tiaraju, nos respectivos municípios de Itapeva/SP e Ribeirão Preto/SP. Iniciado em 2009, este trabalho visa a assessorar e potencializar os trabalhos de transição agroecológica através da aproximação de agricultores assentados com processos diferentes de ocupação da terra, porém com mesmos objetivos de diversificação do sistema produtivo de forma sustentável. Abstract: This work is part of the project "Capacity building for social and environmental projects for sustainable development in rural settlements in the state of São Paulo, a partnership withEmbrapa Environment INCRA, MST and other organizations offarmers settled.Therefore, we propose to present a step in the work being donein the settlements and Itapeva Sepe Tiaraju, in their municipality of Itapeva / SP, Ribeirao Preto / SP. Started in 2009, this work aims to assist and enhance the work through the transitionalagro- ecological approach to resettled farmers with different processes acupaçao of the earth, but with the same objectivesof diversification of the productive system in a sustainable manner

Utilisation du Raltegravir chez des patients infectés par HIV-1 group O

International audienc

A patient with bacteraemia and possible endocarditis caused by a recently-discovered genomospecies of Capnocytophaga: Capnocytophaga genomospecies AHN8471: a case report

<p>Abstract</p> <p>Introduction</p> <p><it>Capnocytophaga </it>are a genus of bacteria that have been found to be the causative organisms in a range of infections, including serious conditions such as bacteraemia, endocarditis and meningitis. This has been especially true amongst those with serious comorbidities and the immunocompromised populations. Although several species are known to cause human disease, historically, laboratories have often not identified isolates to species level due to the unreliable, laborious techniques needed. With the advent of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis, identification to species level is now frequently possible and desirable, as it may provide clues as to the source of infection and its treatment.</p> <p>Case presentation</p> <p>Here we describe a case of bacteraemia and possible endocarditis in a 64-year-old white British man caused by a newly identified genomospecies of <it>Capnocytophaga </it>in a patient subsequently diagnosed with metastatic adenocarcinoma of the oesophagus. The source of the bacteraemia was presumed to be from the patient's own oral flora.</p> <p>Conclusion</p> <p>Our case further confirms the potential for <it>Capnocytophaga </it>to cause systemic infections, highlights the availability and need for identification of isolates to species level and re-emphasises the difficulty in diagnosing <it>Capnocytophaga </it>infections due to their slow growth in the laboratory.</p

The STAR Silicon Strip Detector (SSD)

The STAR Silicon Strip Detector (SSD) completes the three layers of the Silicon Vertex Tracker (SVT) to make an inner tracking system located inside the Time Projection Chamber (TPC). This additional fourth layer provides two dimensional hit position and energy loss measurements for charged particles, improving the extrapolation of TPC tracks through SVT hits. To match the high multiplicity of central Au+Au collisions at RHIC the double sided silicon strip technology was chosen which makes the SSD a half million channels detector. Dedicated electronics have been designed for both readout and control. Also a novel technique of bonding, the Tape Automated Bonding (TAB), was used to fullfill the large number of bounds to be done. All aspects of the SSD are shortly described here and test performances of produced detection modules as well as simulated results on hit reconstruction are given.Comment: 11 pages, 8 figures, 1 tabl

Meningitis due to Fusobacterium necrophorum in an adult

BACKGROUND: Fusobacterium necrophorum may cause a number of clinical syndromes, collectively known as necrobacillosis. Meningitis is a significant cause of mortality, rarely reported in the adult population. CASE PRESENTATION: We report a fatal case of meningitis, caused by Fusobacterium necrophorum, secondary to otitis media in an alcoholic male. Diagnosis was delayed due to the typical slow growth of the organism. The clinical course was complicated by encephalitis and by hydrocephalus. The patient failed to respond to metronidazole and penicillin. The patient died on day 12 from increased intracranial pressure and brain stem infarction. CONCLUSIONS: This case emphasizes the need for a high index of clinical suspicion to make the diagnosis of Fusobacterium necrophorum meningitis. We recommend the use of appropriate anaerobic culture techniques and antimicrobial coverage for anaerobic organisms when the gram stain shows gram negative bacilli