Stabilized Phase Variants of <I>Vibrio cholerae</I> El Tor P-18895

Produced have been stabilized phase variants of V. cholerae El Tor P-18895 (O- and rugose colonies). Frequency of reversion to initial ST-phenotype does not exceed 10 %. Identity of the origin is verified in VNTR. Evaluated has also been their activity by means of the following diagnostic tests: agglutination assay, sensitivity to diagnostic bacteriophages test, and studies of growth behavior in solid nutrient media. Stabilized variants of V. cholerae El Tor P-18895 can be deployed for further investigations of peculiarities of biofilm formation on various surfaces, bacterial resistance to environmental factors, and for the enhancement of methods for isolation of cholera vibrio variants from ambient environment

Evaluation of Biological Properties of New Selective Differential Medium for Cholera Vibrios Isolation Based on the Results of Laboratоry Trials

Previously developed selective differential medium for V. cholerae growth was modernized. The modernized medium called SDMV-M was shown to possess the required sensitivity and germination index. The growth of E. coli was entirely inhibited, that of P. vulgaris was inhibited considerably. The medium possessed good differentiating ability: orange V. cholerae colonies were clearly distinguished from concomitant microorganisms. In the laboratory trials SDMV-M demonstrated some advantages as regards V. cholerae isolation from contaminated faeces in comparison with the reference medium TCBS

STUDY OF COMPOSITION OF PLANT EXTRACTS, POSSESSING ANTIMICROBIAL EFFECT AGAINST VIBRIO CHOLERAE EL TOR, USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

Aim. Study the composition of plant extracts using high-performance liquid chromatography (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor. Materials and methods. Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method. Results. Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V.cholerae El Tor, were determined. Conclusion. The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in extracts, possessing antimicrobial effect against V.cholerae El Tor strains