Detection of genetic material of causative agents of animal viral diseases in blood-sucking dipterans from the Tyumen Region

The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region

Выявление в кровососущих двукрылых насекомых Тюменской области генетического материала возбудителей вирусных болезней животных

The purpose of the research is to test blood-sucking dipterans collected in the Tyumen Region for genetic material of viruses that cause dangerous diseases in live-stock animals.Materials and methods. From May to October 2021, blood-sucking insects of the Diptera order whose species membership was established by tabular keys were collected on pastures and farms in ten Tyumen Region districts. In 60 samples formed from the captured insects according to the taxonomic affiliation and the period and place of collection, the presence of genetic material of the leukemia provirus and the dermatitis nodularis virus of cattle (bovine) and the African swine fever virus was evaluated by polymerase chain reaction (PCR) in real time.Results and discussion. Adult insects collected for the PCR analysis were blood-sucking flies (family Muscidae, genus Stomoxys), mosquitoes (family Culicidae, genus Aedes), midges (family Simuliidae, genera Byssodon and Schoenbaueria), horseflies (family Tabanidae, genera Hybomitra, Tabanus and Haematopota), and biting midges (family Ceratopogonidae, genus Culicoides). As a result of the PCR testing of the samples for the bovine leukemia provirus DNA, 1 out of 13 samples of Stomoxys spp. (7.7%) and 1 of 13 samples of Hybomitra spp. (7.7%) were positive. The bovine leukemia provirus DNA detected in blood-sucking insects indicates the presence of this pathogen in the insect collection area as well as their possible involvement in its spread. Further research is needed on the Stomoxys spp. and Hybomitra spp. vector competence in vivo, considering natural and climatic features of the Tyumen Region.Цель исследований – тестирование кровососущих двукрылых насекомых, собранных на территории Тюменской области, на наличие генетического материала вирусов – возбудителей опасных заболеваний сельскохозяйственных животных.Материалы и методы. В период с мая по октябрь 2021 г. на пастбищах и фермах десяти районов Тюменской области были проведены сборы кровососущих насекомых отряда Diptera, видовая принадлежность которых была установлена по определительным таблицам. В 60 пробах, сформированных из отловленных насекомых в соответствии с таксономической принадлежностью, периодом и местом сбора, оценивали наличие генетического материала провируса лейкоза и вируса нодулярного дерматита крупного рогатого скота (КРС), вируса африканской чумы свиней методом полимеразной цепной реакции (ПЦР) в режиме реального времени.Результаты и обсуждение. Собранные для ПЦР-исследования взрослые особи насекомых были представлены кровососущими мухами (сем. Muscidae, род Stomoxys), комарами (сем. Culicidae, род Aedes), мошками (сем. Simuliidae, роды Byssodon и Schoenbaueria), слепнями (сем. Tabanidae, роды Hybomitra, Tabanus и Haematopota), мокрецами (сем. Ceratopogonidae, род Culicoides). В результате ПЦР-тестирования проб на наличие ДНК провируса лейкоза КРС положительными оказались 1 из 13 проб Stomoxys spp. (7,7%) и 1 из 13 проб Hybomitra spp. (7,7%). Выявление ДНК провируса лейкоза КРС в кровососущих насекомых указывает на присутствие данного возбудителя на территории сбора насекомых, а также на возможное их участие в его распространении. Необходимы дальнейшие исследования векторной компетентности Stomoxys spp. и Hybomitra spp. в естественных условиях с учетом природно-климатических особенностей Тюменской области