Functional characterization and structure-guided mutational analysis of the transsulfuration enzyme cystathionine γ-lyase from toxoplasma gondii

Sulfur-containing amino acids play essential roles in many organisms. The protozoan parasite Toxoplasma gondii includes the genes for cystathionine β-synthase and cystathionine γ-lyase (TgCGL), as well as for cysteine synthase, which are crucial enzymes of the transsulfuration and de novo pathways for cysteine biosynthesis, respectively. These enzymes are specifically expressed in the oocyst stage of T. gondii. However, their functionality has not been investigated. Herein, we expressed and characterized the putative CGL from T. gondii. Recombinant TgCGL almost exclusively catalyses the α,γ-hydrolysis of L-cystathionine to form L-cysteine and displays marginal reactivity toward L-cysteine. Structure-guided homology modelling revealed two striking amino acid differences between the human and parasite CGL active-sites (Glu59 and Ser340 in human to Ser77 and Asn360 in toxoplasma). Mutation of Asn360 to Ser demonstrated the importance of this residue in modulating the specificity for the catalysis of α,β-versus α,γ-elimination of L-cystathionine. Replacement of Ser77 by Glu completely abolished activity towards L-cystathionine. Our results suggest that CGL is an important functional enzyme in T. gondii, likely implying that the reverse transsulfuration pathway is operative in the parasite; we also probed the roles of active-site architecture and substrate binding conformations as determinants of reaction specificity in transsulfuration enzymes

Analysis of Narrow s-channel Resonances at Lepton Colliders

The procedures for studying a single narrow s-channel resonance or nearly degenerate resonances at a lepton collider, especially a muon collider, are discussed. In particular, we examine four methods for determining the parameters of a narrow s-channel resonance: scanning the resonance, measuring the convoluted cross section, measuring the Breit-Wigner area, and sitting on the resonance while varying the beam energy resolution. This latter procedure is new and appears to be potentially very powerful. Our focus is on computing the errors in resonance parameters resulting from uncertainty in the beam energy spread. Means for minimizing these errors are discussed. The discussion is applied to the examples of a light SM-Higgs, of the lightest pseudogoldstone boson of strong electroweak breaking, and of the two spin-1 resonances of the Degenerate BESS model (assuming that the beam energy spread is less than their mass splitting). We also examine the most effective procedures for nearly degenerate resonances, and apply these to the case of Degenerate BESS resonances with mass splitting of order the beam energy spread.Comment: 63 pages, 16 figure

A Strong Electroweak Sector at Future mu^+ mu^- Colliders

We discuss the prospects for detecting at a muon collider the massive new vector resonances V and light pseudo-Nambu-Goldstone bosons P of a typical strongly interacting electroweak sector (as represented by the BESS model). Expected sensitivities to V's at a high energy collider are evaluated and the excellent prospects for discovering P's via scanning at a low energy collider are delineated.Comment: LaTeX, uses aipproc.cls, aipproc.sty, 10 pages, 6 figures, presented at the Workshop on Physics at the First Muon Collider, Fermilab, November 1997, to appear in the Proceedings, some references added and minor changes in the tex

Extension of Tycho catalog for low-extinction windows in the galactic bulge

We present in this work secondary catalogs up to $m_{Val} \sim 13$ based on the Tycho reference frame (ESA, 1997) for 12 selected low-extinction fields towards the galactic bulge. The observations have been performed with the Askania-Zeiss Meridian Circle equiped with a CCD camera, located at the Abrah\~ao de Moraes Observatory (Valinhos, Brazil) and operated by the Institute of Astronomy and Geophysics, S\~ao Paulo University. The presented catalog, though not complete, has been designed to help in intensive search programmes (e.g. microlensing and variable searches) and therefore the selected standards have a high astrometric and photometric ($V$ band, approximately) quality. The mean precisions obtained were $0.0018^{s}$ in $\alpha$, 0.013'' in $\delta$, 0.030 for the standard deviation in magnitude and 0.0042 for the magnitude when weighted with the error bars in each night (in the mean, 42 stars for the catalog of each window). Tables B.1 to B.12 are also available in eletronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html.Comment: 16 pages, 6 figures, A&A Latex style. Published in A&A

Revision of the Italian magnetic database for the Albegna basin(South Tuscany, Italy)

A comparison between ground level total magnetic field intensity anomaly map (F) of Italy and the total intensity aeromagnetic map by ENI/AGIP, had shown that an anomaly pattern for the Albegna basin (South Tuscany), quite evident from ground measurements, doesn’t show in the aeromagnetic map. Ligurian units, made of ophiolite blocks (metagabbros, basalts, serpentinites), intrusives and subordinate volcanic products, all able to trigger a strong magnetic signal, could not be excluded in the area, and for this reason the magnetic anomaly estimated by ground level measurements was not considered unreasonable. In this paper the result of a magnetic survey finalized to verify the authentic existence of such a large magnetic total intensity anomaly in the Albegna basin, is reported. On the basis of the new result, the suspected ground level total intensity anomaly in the Albegna basin, was demonstrated to be non-existent and then the Italian Magnetic Database corrected accordingly. Measurements and procedures that brought to the magnetic elements elaboration and new anomaly maps for Albegna basin, are shown here

Stellar variability in low-extinction regions towards the Galactic Bulge

Intensive monitoring of low-extinction windows towards the galactic bulge has provided in the last years valuable information for studies about the dynamics, kinematics and formation history of this part of the galaxy, mainly by characterizing the bulge stellar populations (Paczy\'nski, 1996). Since 1997, we have been conducting an intensive photometric-astrometric survey of the galactic bulge, with the monitoring of about 120000 stars in 12 windows uniformly distributed in galactic latitude and longitude (Blanco & Terndrup, 1989 e Blanco, 1988) never before submitted to this kind of survey. For this purpose, we have used the IAG/USP CCD Meridian Circle of the Abrah\~ao de Moraes Observatory. The main objective of this work is the identification and classification of variable objects. In this work we present the set up and development of the necessary tools for a project like this and the posterior analysis of our data. We briefly describe the construction of a program to organize and detect variables among the observed stars, including real time alerts (for variations greater than 0.3 magnitudes). The preliminary analysis after the processing of 76 nights of observation yielded 479 variable stars, from which 96.7 % of them are new. We discuss the preliminary classification of this variables, based on: a) the observed amplitude of variation; b) the shape of light curve; c) the expected variable classes among our data and d) the calculated periods, whenever possible. Finally, we discuss the future perspectives for the project and for the applications and analysis of the discovered variable stars.Comment: 10 pages, 14 figures. Accepted by A&A