Special Collections as a catalyst for flexible pedagogical approaches: three case studies

University Special Collections are increasingly being recognised as a valuable pedagogical resource in higher education teaching and learning. The value of historic artefacts as a cross-disciplinary tool to promote higher order thinking processes such as criticality, questioning and narrative construction is well-established in the museum education literature and is gaining increasing attention in teaching and learning development. In this paper, we present three case studies in which we explore the application of Special Collections in a range of learning development contexts, in order to help students engage with their discipline and discipline-specific higher order skills. Our case studies are explorative in the sense of ‘trialling’ the use of historic artefacts in the classroom, to inform our next steps and development of our method. We conclude with our reflections on the process and outcomes of our explorations, to inform our practice and that of other educators looking to apply this method

Izdvajanje, identifikacija i molekularna karakterizacija izolata vrste Pasteurella multocida izdvojenih iz emua (Dromaius novaehollandiae) u državi Gujarat, Indija.

A total of 168 samples (bone marrow, lung tissue, liver and blood clot) were collected from 42 dead emus suspected to have died from fowl cholera. Samples were subjected for cultural isolation on blood agar. Of these, a total of 22 isolates of Pasteurella spp. were isolated and characterized biochemically and identified as P. multocida by PCR. Isolates were later tested for capsular type by multiplex PCR assay and all were found to be of capsular type A. This is the first report on the isolation, identification and molecular characterization of P. multocida from emu in the state of Gujarat, India. This study has revealed that emus are also susceptible to P. multocida (fowl cholera) and can act as a potential carrier of the organism.Ukupno je 168 uzoraka (koštane srži, tkiva plućiju, jetre i krvnih ugrušaka) bilo prikupljeno od 42 emua uginula pod sumnjom na koleru peradi. Uzorci su bili nacijepljeni na krvni agar. Ukupno su bila izdvojena 22 izolata Pasteurella spp. te su biokemijski i lančanom reakcijom polimerazom identificirani kao vrsta P. multocida. Izolati su zatim bili pretraženi na kapsulni tip višestrukom lančanom reakcijom polimerazom te je ustanovljeno da su svi pripadali kapsulnom tipu A. Ovo je prvo izvješće o izdvajanju, identifikaciji i molekularnoj karakterizaciji vrste P. multocida izdvojenoj iz emua u državi Gujarat u Indiji. Istraživanje je pokazalo da je i emu prijemljiv na infekciju vrstom P. multocida, uzročnikom kolere peradi te može biti njezin kliconoša

A comparative study of laparoscopic cholecystectomy with and without abdominal drain

Background: Laparoscopic surgery has several advantages when compared to open surgery, including faster post-operative recovery and lower pain scores. Laparoscopic cholecystectomy is the main method of treatment of symptomatic gallstones. Routine drainage after laparoscopic cholecystectomy is an issue of considerable debate.Methods: Study was randomized, prospective, observational and longitudinal including 100 patients, selected according to inclusion criteria.Results: The sub hepatic fluid collection on first ultrasound at 24hrs was higher in drained group than in non-drained groups. Further, the difference became insignificant on subsequent ultrasound at 72hrs. Incidence of post-operative drain site pain was present in 25% of patients with drain (more at drain site). Incidence drain site infection was present in 16.6% of patients in drain group. Majority of the patients with drain group (n=24) required hospital stay for ≥3 days, while for majority of patients without drain group (n=20), required hospital stay was 1 day.Conclusions: An uncomplicated gall stone disease can be treated by laparoscopic cholecystectomy without need for drain with reasonable safety by an experienced surgeon. With no usage of drain, it is significantly advantageous in terms of post-operative pain, use of analgesics and hospital stay

Izdvajanje, identifikacija i molekularna karakterizacija izolata vrste Pasteurella multocida izdvojenih iz emua (Dromaius novaehollandiae) u državi Gujarat, Indija.

A total of 168 samples (bone marrow, lung tissue, liver and blood clot) were collected from 42 dead emus suspected to have died from fowl cholera. Samples were subjected for cultural isolation on blood agar. Of these, a total of 22 isolates of Pasteurella spp. were isolated and characterized biochemically and identified as P. multocida by PCR. Isolates were later tested for capsular type by multiplex PCR assay and all were found to be of capsular type A. This is the first report on the isolation, identification and molecular characterization of P. multocida from emu in the state of Gujarat, India. This study has revealed that emus are also susceptible to P. multocida (fowl cholera) and can act as a potential carrier of the organism.Ukupno je 168 uzoraka (koštane srži, tkiva plućiju, jetre i krvnih ugrušaka) bilo prikupljeno od 42 emua uginula pod sumnjom na koleru peradi. Uzorci su bili nacijepljeni na krvni agar. Ukupno su bila izdvojena 22 izolata Pasteurella spp. te su biokemijski i lančanom reakcijom polimerazom identificirani kao vrsta P. multocida. Izolati su zatim bili pretraženi na kapsulni tip višestrukom lančanom reakcijom polimerazom te je ustanovljeno da su svi pripadali kapsulnom tipu A. Ovo je prvo izvješće o izdvajanju, identifikaciji i molekularnoj karakterizaciji vrste P. multocida izdvojenoj iz emua u državi Gujarat u Indiji. Istraživanje je pokazalo da je i emu prijemljiv na infekciju vrstom P. multocida, uzročnikom kolere peradi te može biti njezin kliconoša

Role of triple assessment modalities in diagnosis of palpable breast lump

Background: One fourth of women suffer from breast disease in their lifetime. Carcinoma of breast is the second most common cancer in the world. Timely and accurate diagnosis of breast lump with early intervention can be life saving. There are various modalities for the diagnosis of breast lump such as USG, FNAC and Mammography, MRI etc. but none of them is perfect. There are numerous reports suggesting that if the results of clinical assessment, mammography and FNAC are all combined, the diagnostic accuracy is nearly 100%. Furthermore, these techniques also provide information about tumor size, number, extent and grade preoperatively.Methods: Study was randomized, prospective, observational and longitudinal including 100 patients, selected according to inclusion criteria.Results: The sensitivity, specificity and accuracy of triple test in present study were 98.68%, 87.5%, and 96% respectively. Out of 100, 76 patients were confirmed as having benign lesion and 24 patients having malignant lesion by histopathology examination.Conclusions: Results of triple assessment are same as the results by histopathology examination in majority of cases. It is highly accurate, can be used as a confirmatory diagnostic tool for breast lump, thus there is no need to perform diagnostic open biopsy for breast lump

A study of skin closure of surgical wound by subcuticular sutures with polyglactin 910 fast (rapide vicryl) in planned surgery

Background: The skin is the largest and among the most complex organs of the body. Although the skin functions simply as a protective barrier to interface with our environment, its structure and physiology are complex. Suturing is commonly used today as a mean by which wounds are closed to prevent infection and fasten healing with minimal scarring. Cosmetic results of healed wound are also important and as awareness is increasing among general population, they are more concerned with cosmetic scar. Various techniques are developed to give cosmetically better scar, like subcuticular suture, adhesive tapes, staplers, etc.Methods: Study was randomized, prospective, observational and longitudinal including 100 patients, selected according to inclusion criteria.Results: Cosmetic result of the study were good; 87% patients were having good to excellent cosmetic results. And 13% patients were having scar which was cosmetically not good (scar was either hypertrophied or thickened). complication was not significant (only 3% having on post-operative day 5).Conclusions: Surgical wound closure with subcuticular suture with absorbable suture material gives excellent cosmetic results, cheaper and cost-effective compare to other technique of wound closure having similar cosmetic result. As the removal of suture is not required, no follow up visit needed, and it saves the extra time and expenditure of patient

Distinct host cell proteins incorporated by SIV replicating in CD4+ T Cells from natural disease resistant versus non-natural disease susceptible hosts

<p>Abstract</p> <p>Background</p> <p>Enveloped viruses including the simian immunodeficiency virus (SIV) replicating within host cells acquire host proteins upon egress from the host cells. A number of studies have catalogued such host proteins, and a few have documented the potential positive and negative biological functions of such host proteins. The studies conducted herein utilized proteomic analysis to identify differences in the spectrum of host proteins acquired by a single source of SIV replicating within CD4⁺T cells from disease resistant sooty mangabeys and disease susceptible rhesus macaques.</p> <p>Results</p> <p>While a total of 202 host derived proteins were present in viral preparations from CD4⁺T cells from both species, there were 4 host-derived proteins that consistently and uniquely associated with SIV replicating within CD4⁺T cells from rhesus macaques but not sooty mangabeys; and, similarly, 28 host-derived proteins that uniquely associated with SIV replicating within CD4⁺T cells from sooty mangabeys, but not rhesus macaques. Of interest was the finding that of the 4 proteins uniquely present in SIV preparations from rhesus macaques was a 26 S protease subunit 7 (MSS1) that was shown to enhance HIV-1 'tat" mediated transactivation. Among the 28 proteins found in SIV preparations from sooty mangabeys included several molecules associated with immune function such as CD2, CD3ε, TLR4, TLR9 and TNFR and a bioactive form of IL-13.</p> <p>Conclusions</p> <p>The finding of 4 host proteins that are uniquely associated with SIV replicating within CD4⁺T cells from disease susceptible rhesus macaques and 28 host proteins that are uniquely associated with SIV replicating within CD4⁺T cells from disease resistant sooty mangabeys provide the foundation for determining the potential role of each of these unique host-derived proteins in contributing to the polarized clinical outcome in these 2 species of nonhuman primates.</p