Intracellular Cholesterol Lowering as Novel Target for Anti‐Atherosclerotic Therapy

Atherosclerosis and disorders associated with cardiovascular system remain the major problem of modern medicine and the leading cause of mortality in developed countries. According to the current knowledge, atherosclerosis development can begin early in life. Clinically silent early‐stage lesions can be detected in a large population of young adults. Despite substantial progress in the recent years, therapy of atherosclerosis mostly remains limited to plasma lipid profile correction. Moreover, no therapy is currently available for the treatment of asymptomatic early stages of the disease. The existing synthetic drugs could not be used for this purpose, because of the unfavourable risk/benefit ratio and high cost of treatment, which has to be long‐lasting. In this regard, medications based on natural agents with anti‐atherosclerotic activity may offer interesting possibilities. Current research should focus on detection and evaluation of such agents. One of the important tools for anti‐atherosclerotic drug evaluation is a cell‐based model, which allows measurement of intracellular lipid accumulation. Anti‐atherosclerotic activity of various substances can therefore be evaluated by the decrease of intracellular lipid storage. In this chapter, we will discuss the development and application of cellular models based on primary culture of human arterial wall cells that are suitable for detection and measurement of anti‐atherosclerotic activity of various substances. Using these models, several natural agents have been successfully evaluated, which led to the development of pharmaceutical products with anti‐atherosclerotic activity based on botanicals

On a Search for Hidden Photon CDM by a Multi-Cathode Counter

We report on a new technique of a Multi-Cathode Counter (MCC) developed to search for hidden photon (HP) cold dark matter (CDM) with a mass from 5 to 500 eV. The method is suggested in the assumption that HP-photon mixing causes emission of single electrons from a metal cathode if the mass of hidden photon is greater than a work function of the metal. The measured effect from HP should be dependent on work function of the metal and on the structure of electronic shells of the metal used as a cathode. Potentially this can be used for a verification of the results obtained. Some preliminary results for the upper limit for mixing parameter X have been obtained for HP with a mass from 5 eV to 10 keV as a pure illustration of the potential of this technique. The efforts are continued to refine the procedure of data treatment and to improve the work of MCC. A new detector with a more developed design is under construction.Comment: 13 pages, 9 figures; v.2: minor changes/corrections made, following referee's recommendations; accepted for publication in "Advances in High Energy Physics", open special issue. arXiv admin note: text overlap with arXiv:1512.0467