WOUND-HEALING TEST OF PIPER BETLE LEAF EXTRACT AND ALOE VERA IN GEL PREPARATION

Objective: Burns are skin injuries primarily caused by heat, or due to electricity, chemicals, friction, radiation or fire. It is known that Piper betle and Aloe vera are able to accelerate the healing process of burns. The aim of this research was to evaluate the burn-healing activity of a Piper betle L. and Aloe vera combination in a gel preparation.Methods: Carboxymethylcellulose (CMC) at various concentration was used in the formulation of gel. The formulation of the gel was evaluated for an organoleptic observation, measurement of pH, viscosity, and stability test. A burn-healing evaluation was performed in vivo by making a wound with a hot plate on a wistar rat male. Then 600 mg of gel was applied to the wound. After that, the presence of erythema, eschar, edema, and the extent of the burns were observed for 15 d after gel administration.Results: The results showed that the best formulation of gel consisted of 2 % w/w of carboxymethyl cellulose (CMC) 1.1 % w/w of Aloe vera extract, and 3 % w/w of Piper betle extract. The gel preparation of Piper betle and Aloe vera extract could accelerate burn healing, where the healing percentage on the 9th d (53+1.3 %) is higher than the control (21+1.2%) as well as the erythema and eschar, which is lower than the control.Conclusion: The gel preparation of the Piper betle and Aloe vera extract consisting of 2 % w/w, CMC 1.1 % w/w of Aloe vera extract, and 3 % w/w of Piper betle L. extract can accelerate burn healing.Â