Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively

Anther culture response in F1 hybrids of winter wheat [Triticum aestivum L.]

The effect of cold pretreatment of spikes on somatic embryo induction and anther culture response of 25 F₁ winter wheat hybrids was investigated. The efficiency of androgenic embryos was the highest when spikes were incubated at 4°C for 6-9 days. A total of 2242 (73.0%) green and 885 (27.0%) albino plants were obtained from 9900 cultured anthers. Anther culture response in wheat was found to be markedly affected by the genotype of donor plants. The percentage of green plants varied from 0 to 115.7%. A great majority of anther-derived regenerants were haploids (82.35%), while the remaining plants were spontanoeus diploids (13.73%) and aneuploids (3.92%)

Regeneration of oat androgenic plants in relation to induction media and culture conditions of embryo-like structures

The effect of C17 and W14 induction media on the formation of embryo-like structures (ELS) from F3 generation of nine hexaploid oat hybrids was investigated in the study. In all genotypes, the highest number of ELS (0.6 - 12.1/100 anthers) was obtained on C17 medium. The efficiency of plant regeneration on medium 190-2 was tested, in relation to different ELS culture conditions. The highest rate of green plants per 100 ELS (3.3 - 42.4) was produced by incubation at 22oC in the dark for the first two weeks. Among 36 green regenerants, 28 (77.8%) were haploid and 8 (22.2%) were spontaneous doubled haploids, fully fertile. After colchicine treatment of haploid plants, 19 were partially fertile and set from 1 to 15 seed per panicle