30 research outputs found
Structural–Functional Relationship of the Ribonucleolytic Activity of aIF5A from Sulfolobus solfataricus
The translation factor IF5A is a highly conserved protein playing a well-recognized and well-characterized role in protein synthesis; nevertheless, some of its features as well as its abundance in the cell suggest that it may perform additional functions related to RNA metabolism. Here, we have undertaken a structural and functional characterization of aIF5A from the crenarchaeal Sulfolobus solfataricus model organism. We confirm the association of aIF5A with several RNA molecules in vivo and demonstrate that the protein is endowed with a ribonuclease activity which is specific for long and structured RNA. By means of biochemical and structural approaches we show that aIF5A can exist in both monomeric and dimeric conformations and the monomer formation is favored by the association with RNA. Finally, modelling of the three-dimensional structure of S. solfataricus aIF5A shows an extended positively charged surface which may explain its strong tendency to associate to RNA in vivo
Building trust in agribusiness supply chains: A conceptual model of buyer-seller relationships in the seed potato industry in Asia
In the absence of a certified seed system, potato farmers in Asia must purchase replacement seed tubers from an informal seed system. With no third party assurance that the seed tubers purchased are of good quality, the farmer's decision to purchase seeds may be influenced by the long-standing relationships that have been established between buyers and sellers. Trust is the critical determinant of a good buyer-seller relationship. Through maintaining communication and the making of various relationship specific investments, a conceptual model is proposed which suggests that seed suppliers may engage in trust building behavior which should result in the preferred seed supplier enjoying a greater share of the farmer's patronage
Modeling, Managing, Exposing, and Linking Ontologies with a Wiki-based Tool
In the last decade, the need of having effective and useful tools for the creation and the management of linguistic resources significantly increased. One of the main reasons is the necessity of building linguistic resources (LRs) that, besides the goal of expressing effectively the domain that users want to model, may be exploited in several ways. In this paper we present a wiki-based collaborative tool for modeling ontologies, and more in general any kind of linguistic resources, called MoKi. This tool has been customized in the context of an EU-funded project for addressing three important aspects of LRs modeling: (i) the exposure of the created LRs, (ii) for providing features for linking the created resources to external ones, and (iii) for producing multilingual LRs in a safe manner
From Conditional Random Field (CRF) to Rhetorical Structure Theory (RST): incorporating context information in sentiment analysis
This paper investigates a method based on Conditional Random Fields (CRFs) to incorporate sentence structure (syntax and semantics) and context information to identify sentiments of sentences. It also demonstrates the usefulness of the Rhetorical Structure Theory (RST) taking into consideration the discourse role of text segments. Thus, this paper’s aim is to reconsider the effectiveness of CRF and RST methods in incorporating the contextual information into Sentiment Analysis systems. Both methods are evaluated on two, different in size and genre of information sources, the Movie Review Dataset and the Finegrained Sentiment Dataset (FSD). Finally, we discuss the lessons learned from these experimental settings w.r.t. addressing the following key research questions such as whether there is an appropriate type of social media repository to incorporate contextual information, whether extending the pool of the selected features could improve context incorporation into SA systems and which is the best performing feature combination to achieve such improved performance
The IRMUDOSA System at ESWC-2017 Challenge on Semantic Sentiment Analysis
Multi-Domain opinion mining consists in estimating the polarity of a document by exploiting domain-specific information. One of the main issue of the approaches discussed in literature is their poor capability of being applied on domains that have not been used for building the opinion model. In this paper, we present an approach exploiting the linguistic overlap between domains for building models enabling the estimation of polarities for documents belonging to any other domain. The system implementing such an approach has been presented at the third edition of the Semantic Sentiment Analysis Challenge co-located with ESWC 2017. Fuzzy representation of features polarity supports the modeling of information uncertainty learned from training set and integrated with knowledge extracted from two well-known resources used in the opinion mining field, namely Sentic.Net and the General Inquirer. The proposed technique has been validated on a multi-domain dataset and the results demonstrated the effectiveness of the proposed approach by setting a plausible starting point for future work
The NeuroSent System at ESWC-2018 Challenge on Semantic Sentiment Analysis
Multi-domain sentiment analysis consists in estimating the polarity of a given text by exploiting domain-specific information. One of the main issues common to the approaches discussed in the literature is their poor capabilities of being applied on domains which are different from those used for building the opinion model. In this paper, we will present an approach exploiting the linguistic overlap between domains to build sentiment models supporting polarity inference for documents belonging to every domain. Word embeddings together with a deep learning architecture have been implemented for enabling the building of multi-domain sentiment model. The proposed technique is validated by following the Dranziera protocol in order to ease the repeatability of the experiments and the comparison of the results. The outcomes demonstrate the effectiveness of the proposed approach and also set a plausible starting point for future work
The CLAUSY System at ESWC-2018 Challenge on Semantic Sentiment Analysis
With different social media and commercial platforms, users express their opinion about products in a textual form. Automatically extracting the polarity(i.e. whether the opinion is positive or negative) of a user can be useful for both actors: the online platform incorporating the feedback to improve their product as well as the client who might get recommendations according to his or her preferences. Different approaches for tackling the problem, have been suggested mainly using syntactic features. The “Challenge on Semantic Sentiment Analysis” aims to go beyond the word-level analysis by using semantic information. In this paper we propose a novel approach by employing the semantic information of grammatical unit called preposition. We try to derive the target of the review from the summary information, which serves as an input to identify the proposition in it. Our implementation relies on the hypothesis that the proposition expressing the target of the summary, usually containing the main polarity information
The FeatureSent System at ESWC-2018 Challenge on Semantic Sentiment Analysis
The approach described in this paper explores the use of semantic structured representation of sentences extracted from texts for multi-domain sentiment analysis purposes. The presented algorithm is built upon a domain-based supervised approach using index-like structured for representing information extracted from text. The algorithm extracts dependency parse relationships from the sentences containing in a training set. Then, such relationships are aggregated in a semantic structured together with either polarity and domain information. Such information is exploited in order to have a more fine-grained representation of the learned sentiment information. When the polarity of a new text has to be computed, such a text is converted in the same semantic representation that is used (i) for detecting the domain to which the text belongs to, and then (ii), once the domain is assigned to the text, the polarity is extracted from the index-like structure. First experiments performed by using the Blitzer dataset for training the system demonstrated the feasibility of the proposed approach