15 research outputs found
Sn delta-doping in GaAs
We have prepared a number of GaAs structures delta-doped by Sn using the
well-known molecular beam epitaxy growth technique. The samples obtained for a
wide range of Sn doping densities were characterised by magnetotransport
experiments at low temperatures and in high magnetic fields up to 38 T.
Hall-effect and Shubnikov-de Haas measurements show that the electron densities
reached are higher than for other delta-dopants, like Si and Be. The maximum
carrier density determined by the Hall effect equals 8.4x10^13 cm^-2. For all
samples several Shubnikov-de Haas frequencies were observed, indicating the
population of multiple subbands. The depopulation fields of the subbands were
determined by measuring the magnetoresistance with the magnetic field in the
plane of the delta-layer. The experimental results are in good agreement with
selfconsistent bandstructure calculations. These calculation shows that in the
sample with the highest electron density also the conduction band at the L
point is populated.Comment: 11 pages text (ps), 9 figures (ps), submitted to Semicon. Science
Tech
Bak and Bcl-xL Participate in Regulating Sensitivity of Solid Tumor Derived Cell Lines to Mcl-1 Inhibitors
BH3 mimetics represent a promising tool in cancer treatment. Recently, the drugs targeting the Mcl-1 protein progressed into clinical trials, and numerous studies are focused on the investigation of their activity in various preclinical models. We investigated two BH3 mimetics to Mcl-1, A1210477 and S63845, and found their different efficacies in on-target doses, despite the fact that both agents interacted with the target. Thus, S63845 induced apoptosis more effectively through a Bak-dependent mechanism. There was an increase in the level of Bcl-xL protein in cells with acquired resistance to Mcl-1 inhibition. Cell lines sensitive to S63845 demonstrated low expression of Bcl-xL. Tumor tissues from patients with lung adenocarcinoma were characterized by decreased Bcl-xL and increased Bak levels of both mRNA and proteins. Concomitant inhibition of Bcl-xL and Mcl-1 demonstrated dramatic cytotoxicity in six of seven studied cell lines. We proposed that co-targeting Bcl-xL and Mcl-1 might lead to a release of Bak, which cannot be neutralized by other anti-apoptotic proteins. Surprisingly, in Bak-knockout cells, inhibition of Mcl-1 and Bcl-xL still resulted in pronounced cell death, arguing against a sole role of Bak in the studied phenomenon. We demonstrate that Bak and Bcl-xL are co-factors for, respectively, sensitivity and resistance to Mcl-1 inhibition