Strong electron-phonon coupling in delta-phase stabilized Pu

Heat capacity measurements of the delta-phase stabilized alloy Pu-Al suggest that strong electron-phonon coupling is required to explain the moderate renormalization of the electronic density of states near the Fermi energy. We calculate the heat capacity contributions from the lattice and electronic degrees of freedom as well as from the electron-lattice coupling term and find good overall agreement between experiment and theory assuming a dimensionless electron-phonon coupling parameter of order unity, lambda ~ 0.8. This large electron-phonon coupling parameter is comparable to reported values in other superconducting metals with face-centered cubic crystal structure, for example, Pd (lambda ~ 0.7) and Pb (lambda ~ 1.5). Further, our analysis shows evidence of a sizable residual low-temperature entropy contribution, S_{res} ~ 0.4 k_B (per atom). We can fit the residual specific heat to a two-level system. Therefore, we speculate that the observed residual entropy originates from crystal-electric field effects of the Pu atoms or from self-irradiation induced defects frozen in at low temperatures.Comment: 9 pages, 11 figures, to appear in Phys. Rev.

Nutrient Source and Tillage Effects on Maize: II. Yield, Soil Carbon, and Carbon Dioxide Emissions

There is a need to understand the potential benefits of using the biotechnology waste by‐product from manufacturing as a fertilizer replacement in agriculture, by quantifying the economic value for the farmer and measuring the environmental impact. Measuring CO2 emissions can be used to assess environmental impact, including three widely used micrometeorological methodologies: (i) the Bowen Ratio Energy Balance (BREB), (ii) aerodynamic flux‐gradient theory, and (iii) eddy covariance (EC). As a first step in quantifying benefits of applying biotechnology waste in agriculture, a detailed examination of these three methods was conducted to understand their effectiveness in quantifying CO2 emissions for this specific circumstance. The study measured micrometeorological properties over a field planted to maize (Zea mays L. var. indentata ), one plot treated with biotechnology waste applied as a nutrient amendment, and one plot treated with a typical farmer fertilizer practice. Carbon dioxide flux measurements took place over 1 yr, using both BREB and EC systems. The aerodynamic method was used to gap‐fill BREB system measurements, and those flux estimates were compared with estimates produced separately by the aerodynamic and EC methods. All methods found greater emissions over the biotechnology waste application. The aerodynamic method CO2 flux estimates were considerably greater than both the EC and a combined BREB‐aerodynamic approach. During the day, the EC and BREB methods agree. At night, the aerodynamic approach detects and accounts for buildup of CO2 at the surface during stable periods. The BREB systems combined with aerodynamic approaches provide alternate methods to EC in examining micrometeorological properties near the surface

SMARTer Discontinuation Trial Designs for Developing an Adaptive Treatment Strategy

Abstract Objective: Developing evidenced-based practices for the management of childhood psychiatric disorders requires research studies that address how to treat children during both the acute phase of the disorder and beyond. Given the selection of a medication for acute treatment, discontinuation trials are used to evaluate the effects of treatment duration (e.g., time on medication) and/or maintenance strategies following successful acute-phase treatment. Recently, sequential multiple assignment randomized trials (SMART) have been proposed for use in informing sequences of critical clinical decisions such as those mentioned. The objective of this article is to illustrate how a SMART study is related to the standard discontinuation trial design, while addressing additional clinically important questions with similar trial resources. Method: The recently completed Child/Adolescent Anxiety Multimodal Study (CAMS), a randomized trial that examined the relative efficacy of three acute-phase treatments for pediatric anxiety disorders, along with a next logical step, a standard discontinuation trial design, is used to clarify the ideas. This example is used to compare the discontinuation trial design relative to the SMART design. Results: We find that the standard discontinuation trial can be modified slightly using a SMART design to yield high-quality data that can be used to address a wider variety of questions in addition to the impact of treatment duration. We discuss how this innovative trial design is ultimately more efficient and less costly than the standard discontinuation trial, and may result in more representative comparisons between treatments. Conclusions: Mental health researchers who are interested in addressing questions concerning the effects of continued treatment (for different durations) following successful acute-phase treatment should consider SMART designs in place of discontinuation trial designs in their research. SMART designs can be used to address these and other questions concerning individualized sequences of treatment, such as the choice of a rescue treatment in case of postacute phase relapse.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98496/1/cap%2E2011%2E0073.pd

Nutrient Source and Tillage Effects on Maize: I. Micrometeorological Methods for Measuring Carbon Dioxide Emissions

There is a need to understand the potential benefits of using the biotechnology waste by‐product from manufacturing as a fertilizer replacement in agriculture, by quantifying the economic value for the farmer and measuring the environmental impact. Measuring CO2 emissions can be used to assess environmental impact, including three widely used micrometeorological methodologies: (i) the Bowen Ratio Energy Balance (BREB), (ii) aerodynamic flux‐gradient theory, and (iii) eddy covariance (EC). As a first step in quantifying benefits of applying biotechnology waste in agriculture, a detailed examination of these three methods was conducted to understand their effectiveness in quantifying CO2 emissions for this specific circumstance. The study measured micrometeorological properties over a field planted to maize (Zea mays L. var. indentata ), one plot treated with biotechnology waste applied as a nutrient amendment, and one plot treated with a typical farmer fertilizer practice. Carbon dioxide flux measurements took place over 1 yr, using both BREB and EC systems. The aerodynamic method was used to gap‐fill BREB system measurements, and those flux estimates were compared with estimates produced separately by the aerodynamic and EC methods. All methods found greater emissions over the biotechnology waste application. The aerodynamic method CO2 flux estimates were considerably greater than both the EC and a combined BREB‐aerodynamic approach. During the day, the EC and BREB methods agree. At night, the aerodynamic approach detects and accounts for buildup of CO2 at the surface during stable periods. The BREB systems combined with aerodynamic approaches provide alternate methods to EC in examining micrometeorological properties near the surface

Conservation agriculture as a climate change mitigation strategy in Zimbabwe

There is a need to quantify agriculture’s potential to sequester carbon (C) to inform global approaches aimed at mitigating climate change effects. Many factors including climate, crop, soil management practices, and soil type can influence the contribution of agriculture to the global carbon cycle. The objective of this study was to investigate the C sequestration potential of conservation agriculture (CA) (defined by minimal soil disturbance, maintaining permanent soil cover, and crop rotations). This study used micrometeorological methods to measure carbon dioxide (CO2) flux from several alternative CA practices in Harare, central Zimbabwe. Micrometeorological methods can detect differences in total CO2 emissions of agricultural management practices; our results show that CA practices produce less CO2 emissions. Over three years of measurement, the mean and standard error (SE) of CO2 emissions for the plot with the most consistent CA practices was 0.564 ± 0.0122 g CO2 m-2 h-1, significantly less than 0.928 ± 0.00859 g CO2 m-2 h-1 for the conventional tillage practice. Overall CA practices of no-till with the use of cover crops produced fewer CO2 emissions than conventional tillage or fallow

A post-labeling method for multiplexed and multicolored genotyping analysis of SSR, indel and SNP markers in single tube with bar-coded split tag (BStag)

<p>Abstract</p> <p>Background</p> <p>Genotyping analysis using capillary DNA sequencing with fluorescently labeled primer pairs obtained by polymerase chain reaction (PCR) is widely used, but is expensive. The post-PCR labeling method using fluorescently labeled short oligonucleotides and nested PCR of the amplified product obtained from unlabeled primer pairs is a simple and inexpensive alternative. However, previously reported protocols often produced spurious peaks or inconsistent amplification under multiplexed analysis as a result of simultaneous progress of both the amplification and labeling reactions and local homology of the attached tag sequence.</p> <p>Results</p> <p>A set of 16 bp-long oligonucleotide sequences termed bar-coded split tag (BStag), comprising a common basal region, a three-nucleotide 'bar-code' sequence, and a mismatched nucleotide at the middle position were designed for selective post-PCR labeling. The BStag was attached at the 5' end of the forward primer of interest. The melting temperature of the BStag was low enough to separate the labeling reaction from initial PCR amplification, and each sequence was minimally divergent but maintained maximum selectivity. Post-PCR labeling of the amplified product was achieved by extending for three cycles at a lower annealing temperature after the conventional amplification program with the appropriate fluorescently labeled BStag primer. No amplification was confirmed with BStag primers for 12 plant species. The electropherogram of the labeled product obtained using this method was consistent with that of prelabeled primer, except for their apparent size.</p> <p>Conclusions</p> <p>BStag enabled multiplexed post-PCR labeling of simple sequence repeat or insertion/deletion markers with different dyes in a single tube. BStag in conjunction with locus specific oligo and allele specific oligo was also useful for single nucleotide polymorphism analysis. The labeling protocol was simple and no additional operation was required. Single-tube multiplexed post-PCR labeling is useful for a wide variety of genotyping studies with maximal flexibility and minimal costs.</p

C-Terminus Glycans with Critical Functional Role in the Maturation of Secretory Glycoproteins

The N-glycans of membrane glycoproteins are mainly exposed to the extracellular space. Human tyrosinase is a transmembrane glycoprotein with six or seven bulky N-glycans exposed towards the lumen of subcellular organelles. The central active site region of human tyrosinase is modeled here within less than 2.5 Å accuracy starting from Streptomyces castaneoglobisporus tyrosinase. The model accounts for the last five C-terminus glycosylation sites of which four are occupied and indicates that these cluster in two pairs - one in close vicinity to the active site and the other on the opposite side. We have analyzed and compared the roles of all tyrosinase N-glycans during tyrosinase processing with a special focus on the proximal to the active site N-glycans, s6:N337 and s7:N371, versus s3:N161 and s4:N230 which decorate the opposite side of the domain. To this end, we have constructed mutants of human tyrosinase in which its seven N-glycosylation sites were deleted. Ablation of the s6:N337 and s7:N371 sites arrests the post-translational productive folding process resulting in terminally misfolded mutants subjected to degradation through the mannosidase driven ERAD pathway. In contrast, single mutants of the other five N-glycans located either opposite to the active site or into the N-terminus Cys1 extension of tyrosinase are temperature-sensitive mutants and recover enzymatic activity at the permissive temperature of 31°C. Sites s3 and s4 display selective calreticulin binding properties. The C-terminus sites s7 and s6 are critical for the endoplasmic reticulum retention and intracellular disposal. Results herein suggest that individual N-glycan location is critical for the stability, regional folding control and secretion of human tyrosinase and explains some tyrosinase gene missense mutations associated with oculocutaneous albinism type I

Prevalence of nonmedical methamphetamine use in the United States

<p>Abstract</p> <p>Background</p> <p>Illicit methamphetamine use continues to be a public health concern in the United States. The goal of the current study was to use a relatively inexpensive methodology to examine the prevalence and demographic correlates of nonmedical methamphetamine use in the United States.</p> <p>Methods</p> <p>The sample was obtained through an internet survey of noninstitutionalized adults (n = 4,297) aged 18 to 49 in the United States in 2005. Propensity weighting methods using information from the U.S. Census and the 2003 National Survey on Drug Use and Health (NSDUH) were used to estimate national-level prevalence rates.</p> <p>Results</p> <p>The overall prevalence of current nonmedical methamphetamine use was estimated to be 0.27%. Lifetime use was estimated to be 8.6%. Current use rates for men (0.32%) and women (0.23%) did not differ, although men had a higher 3-year prevalence rate (3.1%) than women (1.1%). Within the age subgroup with the highest overall methamphetamine use (18 to 25 year olds), non-students had substantially higher methamphetamine use (0.85% current; 2.4% past year) than students (0.23% current; 0.79% past year). Methamphetamine use was not constrained to those with publicly funded health care insurance.</p> <p>Conclusion</p> <p>Through the use of an internet panel weighted to reflect U.S. population norms, the estimated lifetime prevalence of methamphetamine use among 18 to 49 year olds was 8.6%. These findings give rates of use comparable to those reported in the 2005 NSDUH. Internet surveys are a relatively inexpensive way to provide complimentary data to telephone or in-person interviews.</p