Use of Bactec MGIT 960 ParaTB system for the diagnosis of bovine paratuberculosis

In the present study, Bactec MGIT 960 ParaTB system, a liquid culture system has been used to culture the Mycobacterium avium paratuberculosis (Map). Faecal, milk and serum samples were collected in separate sterile containers from 20 milking crossbred cows, aged above 4 years with a history of chronic diarrhea, weak body condition and weight loss. Five (25.0%, 5/20) animals reacted positively to single intra-dermal Johnin test. Four (20.0%, 4/20) samples each of serum and milk were positive by ELISA. The positive milk and serum samples belonged to the same individuals. Direct PCR could not detect Map DNA in any of the faeces/milk samples. However, 1 out of 20 faecal samples was tagged positive in system on day 19 post culture. Z-N staining, conventional IS900 PCR and real-time PCR confirmed these to be Map

Project advisory committee subcommittee on Forest Genetics

"October 26-27, 1988."Mass clonal propagation of improved conifers: project 3223 / Becwar ... [et al.] ; Biochemistry of clonal propagation: project 3223-2 / Feirer, Johnson ; Mass clonal propagation of improved hardwoods: project 3223-3 / Dinus, Nagmani, Uddin -- Handouts

Chloroplast ultrastructure and gene expression in embryogenic conifer callus

"October, 1987.""This manuscript is based on results obtained in IPC Project 3223 and was presented at the IUFRO Molecular Genetics Working Group Conference in Chalk River, Ontario in June, 1987.

Biochemical differences between embryogenic and nonembryogenic calli of conifers

"May 1988.""This manuscript is based on results obtained in IPC Project 3223 and has been submitted for consideration for publication in Physiologia Plantarum.

Development and characterization of in vitro embryogenic systems in conifers

"September, 1987.""This manuscript was based on results obtained in IPC Project 3223. This paper has been submitted for publication in the proceedings of the International Union of Forestry Research Organization, Somatic Cell Genetics of Woody Plants, Martinus Nijhoff Publ., Dordrecht, The Netherlands.

The mass production of conifer hybrids. Project 3223, report sixteen : a progress report to the members of the Institute of Paper Chemistry

"February 25, 1989.""The Institute of Paper Chemistry, Ronald J. Dinus, Russell P. Feirer, Morris A. Johnson, Nagmani Rangaswamy, Rafique Uddin, Shirley A. Verhagen.

Status report to the Project Advisory Committee on Forest Genetics, October 28-29, 1986

"October 28-29, 1986."Project 3223. Mass Production of Conifers

Mass production of conifer hybrids. Project 3223, report fourteen : a progress report to the members of the Institute of Paper Chemistry

"March 15, 1987.""The Institute of Paper Chemistry, Morris A. Johnson, Russell P. Feirer, Steven Wann, Michael Becwar, Nagmani Rangaswamy, Shirley A. Verhagen, Ronald J. Dinus and Ronald A. Yeske, acting vice president.

Project advisory committee subcommittee on Forest Genetics

"March 30-31, 1988."Mass clonal propagation of improved conifers: project 3223 / Becwar ... [et al.] ; Mass clonal propagation of genetically improved/engineered hardwoods ; Biochemistry of clonal propagation / Feirer, Johnson -- Handouts