Interview with Barbara Mikulski by Brien Williams

Biographical NoteBarbara Ann Mikulski was born on July 20, 1936, and grew up in the Highlandtown neighborhood of East Baltimore, Maryland. She attended Mount Saint Agnes College and received her M.S.W. from the University of Maryland School of Social Work. She became a social worker, community organizer, and Baltimore city councilor, and she made an unsuccessful bid for the U.S. Senate in 1974 before winning election to the U.S. House of Representatives in 1976. After serving in the House for ten years, she ran for the U.S. Senate in 1986, becoming the first elected woman Democratic U.S. senator. She has won numerous re-elections and continued to serve in the Senate as its longest-serving female senator at the time of this interview. SummaryInterview includes discussion of: Mikulski’s run for the Senate in 1986 and Mitchell’s DSCC role in supporting her campaign; Mitchell dancing with Mikulski at a fund raiser; Mikulski’s reception in the Senate as a female senator; Mikulski-Mitchell ‘spousal impoverishment’ amendment; committee assignments during Mikulski’s first term; women in Congress; DSCC Women’s Senate Network; women’s issues worth legislating and fighting for in the Senate; Mitchell’s qualities as a leader; common constituent interests among ‘coastal senators;’ NAFTA; how Mitchell related to women; Mikulski’s reaction to Mitchell’s retirement from the Senate; Mitchell’s legacy as Senate leader

Numerical Study on Hydrogen–Gasoline Dual-Fuel Spark Ignition Engine

Data Availability Statement: This study did not report any data.Copyright © 2022 by the authors. Hydrogen, as a suitable and clean energy carrier, has been long considered a primary fuel or in combination with other conventional fuels such as gasoline and diesel. Since the density of hydrogen is very low, in port fuel-injection configuration, the engine’s volumetric efficiency reduces due to the replacement of hydrogen by intake air. Therefore, hydrogen direct in-cylinder injection (injection after the intake valve closes) can be a suitable solution for hydrogen utilization in spark ignition (SI) engines. In this study, the effects of hydrogen direct injection with different hydrogen energy shares (HES) on the performance and emissions characteristics of a gasoline port-injection SI engine are investigated based on reactive computational fluid dynamics. Three different injection timings of hydrogen together with five different HES are applied at low and full load on a hydrogen–gasoline dual-fuel SI engine. The results show that retarded hydrogen injection timing increases the concentration of hydrogen near the spark plug, resulting in areas with higher average temperatures, which led to NOX emission deterioration at −120 Crank angle degree After Top Dead Center (CAD aTDC) start of injection (SOI) compared to the other modes. At −120 CAD aTDC SOI for 50% HES, the amount of NOX was 26% higher than −140 CAD aTDC SOI. In the meanwhile, an advanced hydrogen injection timing formed a homogeneous mixture of hydrogen, which decreased the HC and soot concentration, so that −140 CAD aTDC SOI implied the lowest amount of HC and soot. Moreover, with the increase in the amount of HES, the concentrations of CO, CO2 and soot were reduced. Having the HES by 50% at −140 CAD aTDC SOI, the concentrations of particulate matter (PM), CO and CO2 were reduced by 96.3%, 90% and 46%, respectively. However, due to more complete combustion and an elevated combustion average temperature, the amount of NOX emission increased drastically.This research received no external funding

Post-release survival rates and welfare of rehabilitated vervet monkeys in Malawi

Research on primate rehabilitation-release (R&R) is limited, and released troop mortality is generally high. We investigated factors affecting survival and welfare of a rehabilitant troop of vervet monkeys (Chlorocebus pygerythrus rufoviridis) released in Malawi in 2016. Using 9 months of pre- and post-release data from the Lilongwe Wildlife Trust (LWT) and linear modeling, survival analysis, and social network analysis, we considered several potential factors influencing survival. The LWT troop survival rate was 36% and results suggest high ranking individuals, juveniles, and highly socially connected individuals were more likely to survive. Mortality patterns suggest released troops may benefit from platform feeders that encourage greater canopy use, more time at the release site before the rainy season when predation is more common, and predator-awareness training. Future studies using behavioral diversity to assess welfare should use detailed ethograms to capture unique behaviors. LWT’s extensive pre- and post-release monitoring provides vital insight into the troop’s survival. Other rehabilitation centers should follow this strategy to help improve primate R&R programs.Accepted manuscrip

Diversity and toxicity of Pseudo-nitzschia species in Monterey Bay : perspectives from targeted and adaptive sampling

Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Harmful Algae 78 (2018): 129-141, doi:10.1016/j.hal.2018.08.006.Monterey Bay, California experiences near-annual blooms of Pseudo-nitzschia that can affect marine animal health and the economy, including impacts to tourism and commercial/recreational fisheries. One species in particular, P. australis, has been implicated in the most toxic of events, however other species within the genus can contribute to widespread variability in community structure and associated toxicity across years. Current monitoring methods are limited in their spatial coverage as well as their ability to capture the full suite of species present, thereby hindering understanding of HAB events and limiting predictive accuracy. An integrated deployment of multiple in situ platforms, some with autonomous adaptive sampling capabilities, occurred during two divergent bloom years in the bay, and uncovered detailed aspects of population and toxicity dynamics. A bloom in 2013 was characterized by spatial differences in Pseudo39 nitzschia populations, with the low-toxin producer P. fraudulenta dominating the inshore community and toxic P. australis dominating the offshore community. An exceptionally toxic bloom in 2015 developed as a diverse Pseudo-nitzschia community abruptly transitioned into a bloom of highly toxic P. australis within the time frame of a week. Increases in cell density and proliferation coincided with strong upwelling of nutrients. High toxicity was driven by silicate limitation of the dense bloom. This temporal shift in species composition mirrored the shift observed further north in the California Current System off Oregon and Washington. The broad scope of sampling and unique platform capabilities employed during these studies revealed important patterns in bloom formation and persistence for Pseudo-nitzschia. Results underscore the benefit of expanded biological observing capabilities and targeted sampling methods to capture more comprehensive spatial and temporal scales for studying and predicting future events.This work was supported by the National Oceanic and Atmospheric Administration (NA11NOS4780055, NA11NOS4780056, NA11NOS4780030) and a fellowship to H. Bowers from the Packard Foundation

Differential Levels of Stress Proteins (HSPs) in Male and Female Daphnia magna in Response to Thermal Stress: A Consequence of Sex-Related Behavioral Differences?

In two independent experiments, we compared: (1) water depth selection (and accompanying temperature selection) by male and female Daphnia magna under different kinds of environmental stress, including the presence of filamentous cyanobacteria, the risk of predation from fish, and the presence of toxic compounds; and (2) sex-dependent production of heat shock proteins (HSP60, 70, and 90) in response to a sudden change in temperature. Male D. magna selected deep water strata, which offer a relatively stable environment, and thereby avoided the threat of predation and the presence of toxic compounds in surface waters. Correlated with this behavior, males reduce their molecular defenses against stress, such as the production of heat shock proteins (HSPs), and do not maintain the physiological machinery that triggers an increase in HSP levels in response to stress. In contrast, female D. magna actively select habitats that offer optimal conditions for growth and production of offspring. Consequently, females are exposed to variable environmental conditions that may be associated with increased stress. To permit survival in these different habitats, D. magna females require molecular mechanisms to protect their cells from rapid changes in stress levels. Thus, they maintain high constitutive levels of the heat shock proteins from HSP 60, 70, and 90 families, and they have the potential to further enhance the production of the majority of these proteins under stress conditions. The results of this study indicate that the separate habitats selected by male and female D. magna result in different patterns of HSP production, leading us to hypothesize that that male and female Daphnia magna adopt different strategies to maximize the fitness of the species

Onconase responsive genes in human mesothelioma cells: implications for an RNA damaging therapeutic agent

<p>Abstract</p> <p>Background</p> <p>Onconase represents a new class of RNA-damaging drugs. Mechanistically, Onconase is thought to internalize, where it degrades intracellular RNAs such as tRNA and double-stranded RNA, and thereby suppresses protein synthesis. However, there may be additional or alternative mechanism(s) of action.</p> <p>Methods</p> <p>In this study, microarray analysis was used to compare gene expression profiles in untreated human malignant mesothelioma (MM) cell lines and cells exposed to 5 μg/ml Onconase for 24 h. A total of 155 genes were found to be regulated by Onconase that were common to both epithelial and biphasic MM cell lines. Some of these genes are known to significantly affect apoptosis (IL-24, TNFAIP3), transcription (ATF3, DDIT3, MAFF, HDAC9, SNAPC1) or inflammation and the immune response (IL-6, COX-2). RT-PCR analysis of selected up- or down-regulated genes treated with varying doses and times of Onconase generally confirmed the expression array findings in four MM cell lines.</p> <p>Results</p> <p>Onconase treatment consistently resulted in up-regulation of IL-24, previously shown to have tumor suppressive activity, as well as ATF3 and IL-6. Induction of ATF3 and the pro-apoptotic factor IL-24 by Onconase was highest in the two most responsive MM cell lines, as defined by DNA fragmentation analysis. In addition to apoptosis, gene ontology analysis indicated that pathways impacted by Onconase include MAPK signaling, cytokine-cytokine-receptor interactions, and Jak-STAT signaling.</p> <p>Conclusions</p> <p>These results provide a broad picture of gene activity after treatment with a drug that targets small non-coding RNAs and contribute to our overall understanding of MM cell response to Onconase as a therapeutic strategy. The findings provide insights regarding mechanisms that may contribute to the efficacy of this novel drug in clinical trials of MM patients who have failed first line chemotherapy or radiation treatment.</p