Identification and functional characterization of a highly divergent N-acetylglucosaminyltransferase I (TbGnTI) in <em>Trypanosoma brucei</em>

Trypanosoma brucei expresses a diverse repertoire of N-glycans, ranging from oligomannose and paucimannose structures to exceptionally large complex N-glycans. Despite the presence of the latter, no obvious homologues of known β1–4-galactosyltransferase or β1–2- or β1–6-N-acetylglucosaminyltransferase genes have been found in the parasite genome. However, we previously reported a family of putative UDP-sugar-dependent glycosyltransferases with similarity to the mammalian β1–3-glycosyltransferase family. Here we characterize one of these genes, TbGT11, and show that it encodes a Golgi apparatus resident UDP-GlcNAc:α3-d-mannoside β1–2-N-acetylglucosaminyltransferase I activity (TbGnTI). The bloodstream-form TbGT11 null mutant exhibited significantly modified protein N-glycans but normal growth in vitro and infectivity to rodents. In contrast to multicellular organisms, where the GnTI reaction is essential for biosynthesis of both complex and hybrid N-glycans, T. brucei TbGT11 null mutants expressed atypical “pseudohybrid” glycans, indicating that TbGnTII activity is not dependent on prior TbGnTI action. Using a functional in vitro assay, we showed that TbGnTI transfers UDP-GlcNAc to biantennary Man(3)GlcNAc(2), but not to triantennary Man(5)GlcNAc(2), which is the preferred substrate for metazoan GnTIs. Sequence alignment reveals that the T. brucei enzyme is far removed from the metazoan GnTI family and suggests that the parasite has adapted the β3-glycosyltransferase family to catalyze β1–2 linkages

The Globular Cluster Systems in the Coma Ellipticals. III: The Unique Case of IC 4051

Using archival \hst WFPC2 data, we derive the metallicity distribution, luminosity function, and spatial structure of the globular cluster system around IC 4051, a giant E galaxy on the outskirts of the Coma cluster core. The metallicity distribution derived from the (V-I) colors has a mean [Fe/H] = -0.3, a near-complete lack of metal-poor clusters, and only a small metallicity gradient with radius; it may, however, have two roughly equal metallicity subcomponents, centered at [Fe/H] ~ 0.0 and -1.0. The luminosity distribution (GCLF) has the Gaussian-like form observed in all other giant E galaxies, with a peak (turnover) at V = 27.8, consistent with a Coma distance of 100 Mpc. The radial profiles of both the GCS and the halo light show an unusually steep falloff which may indicate that the halo of this galaxy has been tidally truncated. Lastly, the specific frequency of the GCS is remarkably large: we find S_N = 11 +- 2, resembling the central cD-type galaxies even though IC 4051 is not a cD or brightest cluster elliptical. A formation model consistent with most of the observations would be that this galaxy was subjected to removal of a large fraction of its protogalactic gas shortly after its main phase of globular cluster formation, probably by its first passage through the Coma core. Since then, no significant additions due to accretions or mergers have taken place.Comment: 24 pp. plus 13 Figures. Postscript file for the complete paper can also be downloaded from http://www.physun.mcmaster.ca/~harris/WEHarris.html. Astron.J., in pres

The FORS Deep Field Spectroscopic Survey

We present a catalogue and atlas of low-resolution spectra of a well defined sample of 341 objects in the FORS Deep Field. All spectra were obtained with the FORS instruments at the ESO VLT with essentially the same spectroscopic set-up. The observed extragalactic objects cover the redshift range 0.1 to 5.0. 98 objects are starburst galaxies and QSOs at z > 2. Using this data set we investigated the evolution of the characteristic spectral properties of bright starburst galaxies and their mutual relations as a function of the redshift. Significant evolutionary effects were found for redshifts 2 < z < 4. Most conspicuous are the increase of the average C IV absorption strength, of the dust reddening, and of the intrinsic UV luminosity, and the decrease of the average Ly alpha emission strength with decreasing redshift. In part the observed evolutionary effects can be attributed to an increase of the metallicity of the galaxies with cosmic age. Moreover, the increase of the total star-formation rates and the stronger obscuration of the starburst cores by dusty gas clouds suggest the occurrence of more massive starbursts at later cosmic epochs.Comment: 24 pages, 25 figures (35 PS files), 4 tables, accepted for publication in A&A. v2: minor typos corrected and references update

The Bulge-Disk Orthogonal Decoupling in Galaxies: NGC 4698

The R-band isophotal map of the Sa galaxy NGC 4698 shows that the inner region of the bulge structure is elongated perpendicularly to the major axis of the disk, this is also true for the outer parts of the bulge if a parametric photometric decomposition is adopted. At the same time the stellar component is characterized by an inner velocity gradient and a central zero-velocity plateau along the minor and major axis of the disk respectively. This remarkable geometric and kinematic decoupling suggests that a second event occurred in the formation history of this galaxy.Comment: 12 pages, LaTex, with 4 PostScript figures. Accepted for publication in The Astrophysical Journal Letter

Mid-infrared colour gradients and the colour-magnitude relation in Virgo early-type galaxies

We make use of Spitzer imaging between 4 and 16 micron and near-infrared data at 2.2 micron to investigate the nature and distribution of the mid-infrared emission in a sample of early-type galaxies in the Virgo cluster. These data allow us to conclude, with some confidence, that the emission at 16 micron in passive ETGs is stellar in origin, consistent with previous work concluding that the excess mid-infrared emission comes from the dusty envelopes around evolved AGB stars. There is little evidence for the mid-infrared emission of an unresolved central component, as might arise in the presence of a dusty torus associated with a low-luminosity AGN. We nonetheless find that the 16 micron emission is more centrally peaked than the near-infrared emission, implying a radial stellar population gradient. By comparing with independent evidence from studies at optical wavelengths, we conclude that a metallicity that falls with increasing radius is the principal driver of the observed gradient. We also plot the mid-infrared colour-magnitude diagram and combine with similar work on the Coma cluster to define the colour-magnitude relation for absolute K-band magnitudes from -26 to -19. Because a correlation between mass and age would produce a relation with a gradient in the opposite sense to that observed, we conclude that the relation reflects the fact that passive ETGs of lower mass also have a lower average metallicity. The colour-magnitude relation is thus driven by metallicity effects. In contrast to what is found in Coma, we do not find any objects with anomalously bright 16 micron emission relative to the colour-magnitude relation. Although there is little overlap in the mass ranges probed in the two clusters, this may suggest that observable ``rejuvenation'' episodes are limited to intermediate mass objects.Comment: 8 pages, 4 figure

The Tully-Fisher relation at intermediate redshift

Using the Very Large Telescope in Multi Object Spectroscopy mode, we have observed a sample of 113 field spiral galaxies in the FORS Deep Field (FDF) with redshifts in the range 0.1<z<1.0. The galaxies were selected upon apparent brightness (R<23) and encompass all late spectrophotometric types from Sa to Sdm/Im. Spatially resolved rotation curves have been extracted for 77 galaxies and fitted with synthetic velocity fields taking into account all observational effects from inclination and slit misalignment to seeing and slit width. We also compared different shapes for the intrinsic rotation curve. To gain robust values of V_max, our analysis is focussed on galaxies with rotation curves which extend well into the region of constant rotation velocity at large radii. If the slope of the local Tully-Fisher relation (TFR) is held fixed, we find evidence for a mass-dependent luminosity evolution which is as large as up to 2 mag for the lowest-mass galaxies, but is small or even negligible for the highest-mass systems in our sample. In effect, the TFR slope is shallower at z~0.5 in comparison to the local sample. We argue for a mass-dependent evolution of the mass-to-light ratio. An additional population of blue, low-mass spirals does not seem a very appealing explanation. The flatter tilt we find for the distant TFR is in contradiction to the predictions of recent semi-analytic simulations.Comment: 18 pages, 14 figures, A&A, in press. Section on sample completeness added. Please note that the entire analysis is based on undisturbed, high quality rotation curves! Potential effects of tidal interactions are also discusse

NGC 770: A Counter-Rotating Core in a Low-Luminosity Elliptical Galaxy

We present evidence for a counter-rotating core in the low-luminosity (M_B = -18.2) elliptical galaxy NGC 770 based on internal stellar kinematic data. This counter-rotating core is unusual as NGC 770 is not the primary galaxy in the region and it lies in an environment with evidence of on-going tidal interactions. We discovered the counter-rotating core via single-slit Keck/ESI echelle spectroscopy; subsequent integral field spectroscopy was obtained with the Gemini/GMOS IFU. The counter-rotating region has a peak rotation velocity of 21 km/s as compared to the main galaxy's rotation speed of greater than 45 km/s in the opposite direction. The counter-rotating region extends to a radius of 4'' (0.6 kpc), slightly smaller than the half-light radius of the galaxy which is 5.3'' (0.8 kpc) and is confined to a disk whose scale height is less than 0.8'' (0.1 kpc). We compute an age and metallicity of the inner counter-rotating region of 3 +/- 0.5 Gyr and [Fe/H] = 0.2 +/- 0.2 dex, based on Lick absorption-line indices. The lack of other large galaxies in this region limits possible scenarios for the formation of the counter-rotating core. We discuss several scenarios and favor one in which NGC 770 accreted a small gas-rich dwarf galaxy during a very minor merging event. If this scenario is correct, it represents one of the few known examples of merging between two dwarf-sized galaxies.Comment: 26 pages, 9 figures. Accepted to AJ. See this http://www.ociw.edu/~mgeha/geha.ps.gz for version with high resolution figure

The formation of the [alpha/Fe] radial gradients in the stars of elliptical galaxies

The scope of this paper is two-fold: i) to test and improve our previous models of an outside-in formation for the majority of ellipticals in the context of the SN-driven wind scenario, by means of a careful study of gas inflows/outflows; ii) to explain the observed slopes, either positive or negative, in the radial gradient of the mean stellar [alpha/Fe], and their apparent lack of any correlation with all the other observables. In order to pursue these goals we present a new class of hydrodynamical simulations for the formation of single elliptical galaxies in which we implement detailed prescriptions for the chemical evolution of H, He, O and Fe. We find that all the models which predict chemical properties (such as the central mass-weighted abundance ratios, the colours as well as the [] gradient) within the observed ranges for a typical elliptical, also exhibit a variety of gradients in the [] ratio, in agreement with the observations (namely positive, null or negative). All these models undergo an outside-in formation, in the sense that star formation stops earlier in the outermost than in the innermost regions, owing to the onset of a galactic wind. The typical [] gradients predicted by our models have a slope of -0.3 dex per decade variation in radius, consistent with the mean values of several observational samples. We can safely conclude that the history of star formation is fundamental for the creation of abundance gradients in ellipticals but that radial flows with different velocity in conjunction with the duration and efficiency of star formation in different galactic regions are responsible for the gradients in the [] ratios.Comment: A&A accepted, replaced with final version after the peer-review proces

The FORS Deep Field: Field selection, photometric observations and photometric catalog

The FORS Deep Field project is a multi-colour, multi-object spectroscopic investigation of an approx. 7 times 7 region near the south galactic pole based mostly on observations carried out with the FORS instruments attached to the VLT telescopes. It includes the QSO Q 0103-260 (z = 3.36). The goal of this study is to improve our understanding of the formation and evolution of galaxies in the young Universe. In this paper the field selection, the photometric observations, and the data reduction are described. The source detection and photometry of objects in the FORS Deep Field is discussed in detail. A combined B and I selected UBgRIJKs photometric catalog of 8753 objects in the FDF is presented and its properties are briefly discussed. The formal 50% completeness limits for point sources, derived from the co-added images, are 25.64, 27.69, 26.86, 26.68, 26.37, 23.60 and 21.57 in U, B, g, R, I, J and Ks (Vega-system), respectively. A comparison of the number counts in the FORS Deep Field to those derived in other deep field surveys shows very good agreement.Comment: 15 pages, 11 figures (included), accepted for publication in A&