Microsatellite diversity and effective population size in a germplasm bank of Hymenaea courbaril var. stilbocarpa (Leguminosae), an andangered tropical tree: recommendations for conservation.

Made available in DSpace on 2018-06-13T00:44:07Z (GMT). No. of bitstreams: 1 SP19753ID31775.pdf: 319689 bytes, checksum: 4102fddb197838c87dc923afabc26672 (MD5) Previous issue date: 2009-09-1

Variabilidade genética em genótipos de feijoeiro comum avaliada com marcadores moleculares.

Baseados em evidências morfológicas e em marcadores bioquímicos (isoenzimas e faseolinas) foram definidos os pools gênicos Mesoamericano e Andino de Phaseolus vulgaris. Os progressos no melhoramento do feijoeiro comum têm sido freqüentemente baixos e pouco decisivos, situação que pode estar ligada à limitada ou estreita variabilidade dos genitores, quase sempre selecionados dentro dos mesmos pools. Visando o estudo da variabilidade genética, 21 cultivares elite dos Ensaios Regionais de Feijão coordenados pela Embrapa Arroz e Feijão, foram caracterizados marcadores moleculares RAPD (Random Amplified Polymorphic DNA). Também os pedigrees das 21 cultivares elite foram pesquisados com o objetivo de estudar o número de genitores envolvidos nos seus desenvolvimentos

Marcadores moleculares ligados a genes de resistência identificados no programa de melhoramento do feijoeiro do BIOAGRO/UFV.

O programa de melhoramento do feijoeiro visando resistência a doenças com auxílio de marcadores moleculares do BIOAGRO/UFV vem sendo conduzido desde 1992, com auxílio do PADCT/FINEP e da FAPEMIG. O programa é baseado em retrocruzamentos visando a piramidação de genes de resistência à antracnose, mancha-angular e ferrugem no cultivar Carioca Rudá (A 285). Na primeira fase do projeto, o programa de melhoramento teve como objetivos determinar o modo de herança dos genes das fontes de resistência a antracnose, ferrugem e mancha angular do feijoeiro causadas pelos fungos Colletotrichum lindemuthianum, Uromyces appendiculatus e Phaeoisariopses griseola, respectivamente, usadas no programa, e identificar marcadores moleculares do tipo RAPD ligados a esses genes

Linkage mapping of the Phg-1 and Co-14 genes for resistance to angular leaf spot and anthracnose in the common bean cultivar AND 277

The Andean common bean AND 277 has the Co-14 and the Phg-1 alleles that confer resistance to 21 and eight races, respectively, of the anthracnose (ANT) and angular leaf spot (ALS) pathogens. Because of its broad resistance spectrum, Co-14 is one of the main genes used in ANT resistance breeding. Additionally, Phg-1 is used for resistance to ALS. In this study, we elucidate the inheritance of the resistance of AND 277 to both pathogens using F2 populations from the AND 277 × Rudá and AND 277 × Ouro Negro crosses and F2:3 families from the AND 277 × Ouro Negro cross. Rudá and Ouro Negro are susceptible to all of the above races of both pathogens. Co-segregation analysis revealed that a single dominant gene in AND 277 confers resistance to races 65, 73, and 2047 of the ANT and to race 63-23 of the ALS pathogens. Co-14 and Phg-1 are tightly linked (0.0 cM) on linkage group Pv01. Through synteny mapping between common bean and soybean we also identified two new molecular markers, CV542014450 and TGA1.1570, tagging the Co-14 and Phg-1 loci. These markers are linked at 0.7 and 1.3 cM, respectively, from the Co-14/Phg-1 locus in coupling phase. The analysis of allele segregation in the BAT 93/Jalo EEP558 and California Dark Red Kidney/Yolano recombinant populations revealed that CV542014450 and TGA1.1570 segregated in the expected 1:1 ratio. Due to the physical linkage in cis configuration, Co-14 and Phg-1 are inherited together and can be monitored indirectly with the CV542014450 and TGA1.1570 markers. These results illustrate the rapid discovery of new markers through synteny mapping. These markers will reduce the time and costs associated with the pyramiding of these two disease resistance genes

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Analysis of the pathogenic variability of Phaeoisariopsis griseola in Brazil.

The main goals of this work was to identify among the Phaeoisariopsis griseola (Sacc.) Ferraris differential cultivars, those with widest resistance spectra for this pathogen in Brazil

Analysis of the pathogenic variability of Colletotrichum lindemuthianum in Brazil.

The main goal of this work was to identify, among the Colletotrichum lindemuthianum differential cultivars, those with most ample resistance spectra for this pathogen in Brazil.Made available in DSpace on 2011-04-09T14:32:03Z (GMT). No. of bitstreams: 1 BIC200403.pdf: 68461 bytes, checksum: fc2b6122785ec569e281fe6f60c16c28 (MD5) Previous issue date: 2004-09-28200

Analysis of the pathogenic variability of Colletotrichum lindemuthianum in Brazil.

The main goal of this work was to identify, among the Colletotrichum lindemuthianum differential cultivars, those with most ample resistance spectra for this pathogen in Brazil