Identifying drivers of leaf water and cellulose stable isotope enrichment in Eucalyptus in northern Australia

Several previous studies have investigated the use of the stable hydrogen and oxygen isotope composi- tions in plant materials as indicators of palaeoclimate. However, accurate interpretation relies on a detailed understanding of both physiological and environmental drivers of the variations in isotopic enrichments that occur in leaf water and associated organic compounds. To pro- gress this aim we measured δ18O and δ2H values in euca- lypt leaf and stem water and δ18O values in leaf cellulose, along with the isotopic compositions of water vapour, across a north-eastern Australian aridity gradient. Here we compare observed leaf water enrichment, along with pre- viously published enrichment data from a similar north Australian transect, to Craig–Gordon-modelled predic- tions of leaf water isotopic enrichment. Our investigation of model parameters shows that observed 18O enrichment across the aridity gradients is dominated by the relation- ship between atmospheric and internal leaf water vapour pressure while 2H enrichment is driven mainly by variation in the water vapour—source water isotopic disequilibrium. During exceptionally dry and hot conditions (RH 37 °C) we observed strong deviations from Craig– Gordon predicted isotope enrichments caused by partial stomatal closure. The atmospheric–leaf vapour pressure relationship is also a strong predictor of the observed leaf cellulose δ18O values across one aridity gradient. Our find- ing supports a wider applicability of leaf cellulose δ18O composition as a climate proxy for atmospheric humidity conditions during the leaf growing season than previously documented

Stable isotopes in leaf water of terrestrial plants

Leaf water contains naturally occurring stable isotopes of oxygen and hydrogen in abundances that vary spatially and temporally. When sufficiently understood, these can be harnessed for a wide range of applications. Here, we review the current state of knowledge of stable isotope enrichment of leaf water, and its relevance for isotopic signals incorporated into plant organic matter and atmospheric gases