Motion of a massive microsphere bound to a spherical vesicle

We study the motion of a small solid particle (a few micrometers in size) attached to the membrane of a spherical giant lipid vesicle. By means of optical manipulation, the particle is first brought near the top of the vesicle, and released. We determine the friction experienced by the particle moving along the vesicle surface under the action of gravity. From experiments with latex and glass beads, we check that SOPC membranes are fluid at room temperature (static shear modulus u = 0) and estimate the shear viscosity of SOPC bilayers: nm = 3 * 10^(-6) surface poise

Motion of a massive microsphere bound to a spherical vesicle

We study the motion of a small solid particle (a few micrometers in size) attached to the membrane of a spherical giant lipid vesicle. By means of optical manipulation, the particle is first brought near the top of the vesicle, and released. We determine the friction experienced by the particle moving along the vesicle surface under the action of gravity. From experiments with latex and glass beads, we check that SOPC membranes are fluid at room temperature (static shear modulus $\mu=0$) and estimate the shear viscosity of SOPC bilayers: $\eta_{\rm m}\approx 3\cdot 10^{-6}$ surface poise

Falling ball viscosimetry of giant vesicle membranes: Finite-size effects

We study the general problem of the friction felt by a spherical solid particle which moves parallel to the membrane of a spherical vesicle. Experiments are carried out with SOPC vesicles at room temperature, with different particle and vesicle sizes. Experimental data show considerable finite-size effects whenever the particle is not very small compared to the vesicle. These effects are found consistent with the hydrodynamical theory of the vesicle-particle problem. This agreement allows for a "robust" determination of membrane viscosity, independently of particle and vesicle sizes