Delayed hepatic uptake of multi-phosphonic acid poly(ethylene glycol) coated iron oxide measured by real-time Magnetic Resonance Imaging

We report on the synthesis, characterization, stability and pharmacokinetics of novel iron based contrast agents for magnetic resonance imaging (MRI). Statistical copolymers combining multiple phosphonic acid groups and poly(ethylene glycol) (PEG) were synthesized and used as coating agents for 10 nm iron oxide nanocrystals. In vitro, protein corona and stability assays show that phosphonic acid PEG copolymers outperform all other coating types examined, including low molecular weight anionic ligands and polymers. In vivo, the particle pharmacokinetics is investigated by monitoring the MRI signal intensity from mouse liver, spleen and arteries as a function of the time, between one minute and seven days after injection. Iron oxide particles coated with multi-phosphonic acid PEG polymers are shown to have a blood circulation lifetime of 250 minutes, i.e. 10 to 50 times greater than that of recently published PEGylated probes and benchmarks. The clearance from the liver takes in average 2 to 3 days and is independent of the core size, coating and particle stability. By comparing identical core particles with different coatings, we are able to determine the optimum conditions for stealth MRI probes.Comment: 19 pages 8 figures, RSC Advances, 201

Vanillin-Based Photocurable Anticorrosion Coatings Reinforced with Nanoclays

This study investigates the chemical-physical properties and anticorrosion effectiveness of UV-cured coatings produced using epoxidized vanillin (DGEVA) as biobased precursor, then reinforced by the addition of nanoclay. After optimizing the UV-curing parameters of three different formulations by Fourier transform infrared spectroscopy (FTIR), the thermo-mechanical properties of the coatings are assessed by differential scanning calorimetric analysis (DSC), dynamic thermal mechanical analysis (DTMA), and pencil hardness. The coatings are applied on mild steel substrates and then their barrier properties are investigated by electrochemical impedance spectroscopy measurements, immersing the samples in 3.5 wt% NaCl aerated solutions. The results show the good corrosion protective effectiveness of the biobased coatings. The nanoclay addition has a beneficial effect, as it hinders the diffusion of the aggressive ions from the electrolyte solution to the metal substrate. The reported findings demonstrate the possibility of using biobased precursors and UV-curing technology to reduce the environmental impact of the coating industry.A photocurable epoxy composite is realized using epoxidized vanillin (DGEVA) as a biobased precursor, and functionalized nanoclay as filler. The coating shows good thermo-mechanical properties, high chemical resistance, and satisfactory corrosion protection effectiveness in aggressive environments containing chlorides. imag

Preventing corona effects: multi-phosphonic acid poly(ethylene glycol) copolymers for stable stealth iron oxide nanoparticles

When disperse in biological fluids, engineered nanoparticles are selectively coated with proteins, resulting in the formation of a protein corona. It is suggested that the protein corona is critical in regulating the conditions of entry into the cytoplasm of living cells. Recent reports describe this phenomenon as ubiquitous and independent of the nature of the particle. For nanomedicine applications however, there is a need to design advanced and cost-effective coatings that are resistant to protein adsorption and that increase the biodistribution in vivo. In this study, phosphonic acid poly(ethylene glycol) copolymers were synthesized and used to coat iron oxide particles. The copolymer composition was optimized to provide simple and scalable protocols as well as long-term stability in culture media. It is shown that polymers with multiple phosphonic acid functionalities and PEG chains outperform other types of coating, including ligands, polyelectrolytes and carboxylic acid functionalized PEG. PEGylated particles exhibit moreover exceptional low cellular uptake, of the order of 100 femtograms of iron per cell. The present approach demonstrates that the surface chemistry of engineered particles is a key parameter in the interactions with cells. It also opens up new avenues for the efficient functionalization of inorganic surfaces.Comment: 21 page, 7 figures,Biomacromolecules 201

UV-crosslinked Polymeric Materials for Encapsulation of ZnO Nanowires in Piezoelectric Fingerprint Sensors

The work presented here describes new UV-crosslinkable thin layer polymeric materials for the encapsulation of ZnO nanowires (NWs) in multi-NWs pressure based fingerprint sensors. Such innovative sensor is a novel technology for fingerprint capture developed within the PiezoMAT FP7 European project. The sensing principle is based on the piezoelectric property of ZnO NWs, on which a potential difference is generated when they undergo compression and/or bending forces. Since the pressure induced by the finger cannot be directly applied on the NWs, the deformation is applied through a polymeric material that aims at transferring forces from the finger onto the array of NWs without altering their integrity. Besides, since it is dedicated to be in direct contact with human finger or oil pollutants, the encapsulation layer must also exhibit chemical inertness, as well as hydrophobicity and oleophobicity

Piezo-force and vibration analysis of ZnO nanowire arrays for sensor application

To estimate the potential of ZnO nanostructures for force sensing applications, arrays of single nanowires and arrays of nanowire bundles have been fabricated by wet chemical growth method. The piezoelectrical and electrical properties of the single nanowires have been investigated by atomic force microscopy based techniques. The piezoelectric constant d(33) = 15 pm/V has been determined from vibration analyses. The electrical response in the range up to 400 fA upon applying force between 40 nN and 1 mu N has been recorded. The nanowire bundles were studied by electro-mechanical macro probing technique within the force range 1 - 10 mN, where a reproducible response in pA range has been measured

Tracing baculovirus AcMNPV infection using a real time method based on ANCHORTM DNA labeling technology

Many steps in the baculovirus life cycle, from initial ingestion to the subsequent infection of all larval cells, remain largely unknown; primarily because it has hitherto not been possible to follow individual genomes and their lineages. Use of ANCHORTM technology allows a high intensity fluorescent labelling of DNA. When applied to a virus genome, it is possible to follow individual particles, and the overall course of infection. This technology has been adapted to enable labelling of the baculovirus Autographa californica Multiple NucleoPolyhedroVirus genome, as a first step to its application to other baculoviruses. AcMNPV was modified by inserting the two components of ANCHORTM: a specific DNA-binding protein fused to a fluorescent reporter, and the corresponding DNA recognition sequence. The resulting modified virus was stable, infectious, and replicated correctly in Spodoptera frugiperda 9 (Sf9) cells and in vivo. Both budded viruses and occlusion bodies were clearly distinguishable, and infecting cells or larvae allowed the infection process to be monitored in living cells or tissues. The level of fluorescence in the culture medium of infected cells in vitro showed a good correlation with the number of infectious budded viruses. A cassette that can be used in other baculoviruses has been designed. Altogether our results introduce for the first time the generation of autofluorescent baculovirus and their application to follow infection dynamics directly in living cells or tissues

Rivestimenti epossidici fotopolimerizzati derivati da oli vegetali = UV-cured epoxy coatings from vegetable oils

This study presents the characterization of innovative epoxy coatings derived from vegetable oils. They are photopolymerized materials derived from epoxidized grape seed oils or epoxidized rose hip seed oils. A complete characterization of the coatings was performed, both to study chemico-physical properties such as contact angle and glass transition temperature, and to investigate their corrosion protection effectiveness. The coatings were deposited on mild steel substrates and then characterized by means of Electrochemical Impedance Spectroscopy (EIS) in order to analyse their barrier properties. A good protection effectiveness was found, specifically for the coatings characterized by a higher crosslinking density. Thus, results show the possibility to optimize the barrier properties of these bio-based materials by means of a proper choice of the precursor oils

Polymer coated cerium oxide nanoparticles as oxidoreductase-like catalysts

Cerium oxide nanoparticles have been shown to mimic oxidoreductase enzymes by catalyzing the decomposition of organic substrates and reactive oxygen species. This mimicry can be found in superoxide radicals and hydrogen peroxides, harmful molecules produced in oxidative stress asso-ciated diseases. Despite the fact that nanoparticle functionalization is mandatory in the context of nanomedicine, the influence of polymer coatings on their enzyme-like catalytic activity is poorly understood. In this work, six polymer coated cerium oxide nanoparticles are prepared by associa-tion of 7.8 nm cerium oxide cores with two poly(sodium acrylate) and four poly(ethylene glycol) (PEG) grafted copolymers with different terminal or anchoring end groups, such as phosphonic acids. The superoxide dismutase-, catalase-, peroxidase- and oxidase-like catalytic activities of the coated nanoparticles were systematically studied. It is shown that the polymer coatings do not af-fect the superoxide dismutase-like, impair the catalase-like and oxidase-like and surprisingly im-proves peroxidase-like catalytic activities of cerium oxide nanoparticles. It is also demonstrated that the particles coated with the PEG-grafted copolymers perform better than the poly(acrylic acid) coated ones as oxidoreductase-like enzymes, a result that confirms the benefit of having phosphon-ic acids as anchoring groups at the particle surface.Comment: 23 pages, 8 figures, 3 table