528 research outputs found
Contractions from to the M-theory superalgebra extended by additional fermionic generators
We study here the generalized Weimar-Woods contractions of the superalgebra
in order to obtain a suitable algebra that could
describe the gauge group of supergravity. The contracted superalgebras
are assumed to be given in terms of fermionic extensions of the M-theory
superalgebra. We show that the only superalgebra of this type obtained by
contraction is the only one for which the three-form of supergravity
cannot be trivialized. Therefore, supergravity cannot be connected in
this way with a contraction of
Web-based learning and teaching resources for microscopic detection of human parasites.
DMU e-Parasitology (http://parasitology.dmu.ac.uk) presents novel web-based resources co-developed by EU academics at De Montfort University (DMU) for the teaching and learning of microscopic diagnoses of common and emerging human parasites. The package will be completed early in 2019 and presents a Virtual Laboratory and Microscope, which are equipped with engaging units for learning parasitological staining and fresh preparation techniques for detecting cysts, oocysts, eggs and spores, in conjunction with a library of digitised clinical slides. Units are equipped with short videos of academics performing the different techniques and quizzes and exercises, to provide students with the most practical experience possible
Virtual libraries of tissue and clinical samples: potential role of a 3-D microscope.
Our international innovative teaching group from different European Universities (De Montfort University, DMU, UK; and the Spanish University of Alcalá, University Miguel Hernández and University of San Pablo CEU), in conjunction with practicing biomedical scientists in the National Health Service (UK) and biomedical researchers, are developing two complete e-learning packages for teaching and learning medical parasitology, named DMU e-Parasitology (accessible at: http://parasitology.dmu.ac.uk), and biology and chemistry, named DMU e-Biology (accessible at: http://parasitology.dmu.ac.uk/ebiology/index.htm), respectively. Both packages will include a virtual microscope with a complete library of digitised tissue images, clinical slides and cell culture slides/mini-videos for enhancing the teaching and learning of a myriad of techniques applicable to health science undergraduate and postgraduate students. Thus, these packages include detecting human parasites, by becoming familiar with their infective structures and/or organs (e.g. eggs, cysts) and/or explore pathogenic tissues stained with traditional (e.g. haematoxylin & eosin) or more modern (e.g. immunohistochemistry) techniques. The Virtual Microscope (VM) module in the DMU e-Parasitology package is almost completed (accessible at: http://parasitology.dmu.ac.uk/learn/microscope.htm) and contains a section for the three major groups of human-pathogenic parasites (Peña-Fernández et al., 2018) [1]. Digitised slides are provided with the functionality of a microscope by using the gadget Zoomify®, and we consider that they can enhance learning, as previous studies reported in the literature have reported similar sensitivity and specificity rates for identification of parasites for both digitised and real slides. The DMU e-Biology’s VM, currently in development, will provide healthy and pathological tissue samples from a range of mammalian tissues and organs.
This communication will provide a description of both virtual libraries and the process of developing them. In conjunction, we will use a three-dimensional (3D) super-resolution microscopy, 3D Cell Explorer (Nanolive, Lausanne, Switzerland), to incorporate potential 3D microscopic photographs/short videos of cells to provide students with information about the spatial arrangement and morphologies of cells that are essential for life
Cryptosporidium spp. in the English urban environment: a public health concern?
Several species of Cryptosporidium can infect humans and have been described as opportunistic parasites. Different outbreaks have been described in the UK as oocysts of these pathogens can spread through contaminated water and food as some species of Cryptosporidium, such as C. parvum, exhibit resistance to harsh environmental conditions. These pathogens have been found in animal faeces, thus we reported the presence of Cryptosporidium spp. in a dog faecal sample collected in a highly frequented public park in Leicester city centre (UK), after screening 9 topsoil and 18 faecal samples. As a result, and to determine potential risks to the Leicester population, we collected 132 animal faecal samples [37 deer, 13 dogs, 4 cats and 78 avian (27 uncertain due to diarrhoea, 25 pigeon, 14 waterfowl and 12 songbird)] across different parks in Leicester from June 2017 to May 2018. Animal faecal samples were appropriately screened using Kinyoun's acid-fast staining. We observed structures related to Cryptosporidium spp. in 16 faecal samples as follows: 10.3% avian (3 pigeon, 2 songbird and 3 diarrhoeic), 18.9% deer and 7.7% dog. However, and in order to characterise the risks to the local population, molecular analysis will be required to determine if the oocysts of Cryptosporidium spp. found are from anthroponotic species. Our results might highlight the relevance of performing environmental monitoring studies to determine the presence of these pathogens in the urban environment due to the unprecedented expansion of the urban media that is occurring to a global scale
Introducing medical parasitology at the University of Makeni, Sierra Leone
The file attached to this record is the author's final peer reviewed version.Capacity building in Sierra Leone (West Africa) is critical to prevent potential future outbreaks similar to
the 2013-16 Ebola outbreak that had devastating effects for the country and its poorly developed
healthcare system. De Montfort University (DMU) in the United Kingdom (UK), in collaboration with
parasitologists from the Spanish Universities of San Pablo CEU and Miguel Hernández de Elche, is
leading a project to build the teaching and research capabilities of medical parasitology at the University
of Makeni (UniMak, Sierra Leone). This project has two objectives: a) to introduce and enhance the
teaching of medical parasitology, both theoretical and practical; and b) to implement and develop
parasitology research related to important emerging human parasites such as Cryptosporidium spp. due
to their public health significance. Two UniMak academics, hired to help initiate and implement the
research part of the project, shared their culturally sensitive public health expertise to broker parasitology
research in communities and perform a comprehensive environmental monitoring study for the detection
of different emerging human parasites. The presence of targeted parasites are being studied
microscopically using different staining techniques, which in turn have allowed UniMak’s academics to
learn these techniques to develop new practicals in parasitology. To train UniMak’s academics and
develop both parts of our project, a DMU researcher visited UniMak for two weeks in April 2019 and
provided a voluntary short training course in basic parasitology, which is currently not taught in any of
their programmes, and was attended by 31 students. These sessions covered basic introduction to
medical parasitology and life-cycle, pathogenesis, detection, treatment and prevention of: a) coccidian
parasites (Cryptosporidium, Cyclospora and Cystoisospora); b) Giardia intestinalis, Entamoeba and
free-living amoebas; c) malaria and d) microsporidia. A theoretical session on common staining
techniques was also provided. To facilitate the teaching and learning of these parasites, the novel
resource DMU e-Parasitology was used, a package developed by the above participating universities
and biomedical scientists from the UK National Health Service (NHS): http://parasitology.dmu.ac.uk/
index.htm. Following the two weeks of training, UniMak’s academics performed different curriculum
modifications to the undergraduate programme ‘Public Health: Medical Laboratory Sciences’, which
includes the introduction of new practicals in parasitology and changes to enhance the content of
medical parasitology that will be subjected to examination. Thus, a new voluntary practical on Kinyoun
stain for the detection of coccidian parasites was introduced in the final year module of ‘Medical
Bacteriology and Parasitology’; eighteen students in pairs processed faecal samples from pigs provided
by the Department of Agriculture and Food Security from a nearby farm. Academics at UniMak used the
Kinyoun staining unit (available at http://parasitology.dmu.ac.uk/learn/lab/Kinyoun/story_html5.html; [1])
to deliver this practical. Although our project is at a preliminary stage, it has been shown to be effective
in promoting the introduction and establishment of medical parasitology at UniMak and could be viewed
as a case-study for other universities in low-income countries to promote the United Nations (UN)
Sustainable Development Goals (SDGs) and improve public health understanding of infectious
diseases
Human-related microsporidia in the English urban environment: what we know so far?
The potential zoonotic transmission route of Enterocytozoon bieneusi and Encephalitozoon spp. (E. intestinalis, E. hellem and E. cuniculi) is under discussion. “Urban animals” could represent a risk to the population that should be carefully studied. Following the detection of human-related microsporidia in 2 out of 18 dog faecal samples collected in the city centre of Leicester (UK) in January 2016, 228 animal faecal samples were randomly sampled from different parks and recreational areas across the city from June 2016 to February 2017. The presence of microsporidia species were sought by real time polymerase chain reaction (RT-PCR) with melting curve analysis after extracting DNA by dysrupting the spores using Fast-Prep for soil following previous methodologies. 28 stool samples were positive for human-pathogenic microsporidia, as follows: Enterocytozoon bieneusi was detected in 2 waterfowl stool samples. A higher prevalence of Encephalitozoon spp. was identified. Thus, 26 faecal samples (14 deer, 7 avian [3 waterfowl, 2 songbird, 1 pigeon, 1 uncertain], 2 dog, 1 fox and 2 unidentified due to diarhoea) were positive for Encephalitozoon spp. Specifically, E. intestinalis/ E. hellem were detected in two samples from avian species (waterfowl and diarrhoeic sample) and E. cuniculi in 7 (6 deer and 1 fox). Our results indicate that human-related microsporidia would be present in urban parks and recreational areas in Leicester, which could represent a risk for humans. Interventions to tackle this potential risk should be applicable to a variety of animals, although more studies are needed to fully understand the potential zoonotic role of these pathogens
Evaluation of a novel digital environment for learning medical parasitology.
open access articleEukaryotic parasites represent a serious human health threat requiring health professionals with parasitology skills to counteract this threat. However, recent surveys highlight an erosion of teaching of parasitology in medical and veterinary schools, despite reports of increasing instances of food and water borne parasitic infections. To address this we developed a web-based resource, DMU e-Parasitology®, to facilitate the teaching and learning of parasitology, comprising four sections: theoretical; virtual laboratory; virtual microscopy; virtual clinical case studies. Testing the package was performed using a questionnaire given to ninety-five Pharmacy students in 2017/18 to assess effectiveness of the package as a teaching and learning tool. 89.5% of students reported appropriate acquisition of knowledge of the pathology, prevention and treatment of some parasitic diseases. 82.1% also welcomed the clinical specialism of the package as it helped them to acquire basic diagnostic skills, through learning infective features/morphology of the parasites
Studying the presence of Cyclospora and Cystoisospora in urban parks from Leicester, UK.
Cyclospora cayetanensis and Cystoisospora belli (formerly known as Isospora belli) are emerging coccidian parasites that can spread by ingesting contaminated food or water. Despite their presence is more common in tropical and subtropical regions, different studies have described domestic outbreaks due to these pathogens around the world. Zoonotic transmission of these pathogens is under discussion as they have been found in various animals and birds. We have performed a preliminary study to investigate their potential presence in an English urban environment. 132 animal faecal samples were collected between Summer 2017 and Spring 2018 from 7 different urban parks
across Leicester (UK). A veterinarian confirmed animal species as: 78 avian (25 pigeon, 14 waterfowl, 12 songbird, 27 uncertain due to diarrhoea), 37 deer, 13 dogs and 4 cats. Smears were microscopically analysed by Kinyoun's acid-fast staining technique. Cyclospora spp. were observed in three faecal samples (2.3%), two from deer and one from avian (diarrheic sample); however, further analysis are required to determine if the oocysts observed are from Cyclospora cayetanensis. Contrarily, Cystoisospora spp. were not found in any of the screened stool samples. Despite our results should be considered as preliminary, the presence of Cyclospora spp. oocysts in 2.3% of the animal faecal
samples collected across Leicester might represent a potential human risk that, although minor, should be throughly studied to protect the local community. Moreover, Cyclospora spp. have been found in different animal species, which may require different interventions to target those specific animals to protect the public health
Presence of Giardia in urban parks from Leicester, UK
Giardia duodenalis (syn. G. lamblia and G. intestinalis) is a worldwide leading diarrheagenic human protozoan parasite with potential to contaminate water, food or fomites. Animal-to‑animal and animal‑to‑human transmissions are major concerns, however human giardiasis in the United Kingdom (UK) are underdiagnosed. The main aim of this study was to evaluate if Giardia is present in an English urban environment to determine if public health interventions were necessary to protect human health. Thus, 21 fresh animal faecal samples were collected from Humberstone Park in Leicester (UK) in August 2017, when there had been no precipitation for at least the preceding 48 h. A veterinarian identified the possible animal species as: 7 avian (2 songbird, 1 pigeon, 4 uncertain) and 14 canine (dog). Smears were stained with Trichrome as described elsewhere. Giardia spp. cysts were observed in three faecal samples from dogs (21.4%), confirming our previous results in which we detected the presence of G. duodenalis in one dog faecal sample collected in the Leicester’s Castle Park (central Leicester) in a pilot study performed in winter 2016 using the immunoassay ImmunoCard STAT!®. Our results, although preliminary, indicate the presence of Giardia spp. in Leicester’s urban environment. Moreover, dogs could act as reservoirs and might play a potential role in the transmission of these pathogens in Leicester. Thus, public parks could be an important source of infection, particularly for children, as they can accidentally ingest this parasite from soil contaminated with dog faeces. This pilot study has provided critical insight for performing a more comprehensive study to determine the size of this risk and the necessity of enhancing public health awareness to reduce canine faecal pollution in Leicester to protect human health
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