PREVALENCE AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM β-LACTAMASES IN KLEBSIELLA PNEUMONIAE ISOLATES FROM CANCER PATIENTS AND OTHERS

Objective: Klebsiella pneumoniae is highly prevalent in hospitals and causes many nosocomial infections. The study sought to determine prevalence rates of extended spectrum β-lactamases (ESBLs) in clinical isolates of K. pneumoniae from Cairo, Egypt and to detect the ESBL-encoding genes in the isolates.Methods: K. pneumoniae isolates were collected through two-year period (2011-2012). Identification of K. pneumoniae was carried out using automated Microscan and standard biochemical tests. ESBL pattern and minimum inhibitory concentrations (MICs) were detected using Clinical and Laboratory Standards Institute guidelines and confirmatory tests. Multiplex polymerase chain reaction for ESBL-encoding genes and plasmid profiling were performed.Results: In the present work; 112 isolates, 75 of them from cancer patients, were characterized. High proportion (52 of 112, 46 %â€) of ESBLs among the isolates were detected. Highest prevalence of ESBLs was seen among cancer patients, 39 isolates of 75 (52%). Plasmid profile for ESBL-producing K. pneumoniae isolates showed different sizes and numbers of plasmids in all isolates. MICs for all ESBL-producing isolates revealed high resistance rates with tetracycline (100%), cefepime (96%), gentamycin (90%) and ciprofloxacin (79%). Whereas, only two isolates (4%) were resistant to both carbapenem drugs tested, imipenem and meropenem. blaTEM, blaSHV, and bla CTX-M were performed for all ESBL-producing isolates. Five patterns of ESBL-encoding genes were detected. The most prevalent ESBL-encoding gene was blaTEM;alone in 40% and with other ESBL-encoding gene(s) in 48% of the isolates.Conclusion: High prevalence of ESBL (46%) in our isolates suggesting the need for continuous monitoring of emergence of this pattern in our region.Â

Interaction of CO molecules with (Cu , Ag and Au) deposited on regular and defective MgO and BaO(001) surfaces: Density functional calculations

The adsorption properties and characteristics of CO on Cu, Ag and Au atoms deposited on various sites of the alkaline earth oxide MgO and BaO. The three members of morphological irregularities, terrace, edge, and oxygen terminated corner of MgO and BaO (001) surface have been studied by means of density functional calculations and embedded cluster model. The examined clusters were embedded in the simulated Coulomb fields that closely approximate the Madelung fields of the host surfaces. The  adsorption  properties  of  CO  have  been  analyzed  with reference  to  the  nature  of  the  oxide  support,  pairwise  and  non-pairwise  ..........Please read the full paper

BIOAVAILABILITY STUDY OF ONDANSETRON GEL IN RABBITS AND HUMAN VOLUNTEERS APPLING UPLC AS ANALYTICAL TOOL AND EVALUATION OF THE ANTIEMETIC EFFECT OF ONDANSETRON GEL IN CISPLATIN-INDUCED EMESIS IN RATS

Objective: This study was undertaken to determine the bioavailability of ondansetron gel in experimental animals and humans applying UPLC as an analytical tool and evaluation of the antiemetic effect of ondansetron gel in cisplatin-induced emesis in rats. Methods: Ondansetron gel (F13: sodium alginate 7% w/w) was used, marketed I. V. ondansetron (Zofran) ® was chosen as reference. The bioavailability study in rabbits was selected as a parallel design using nine healthy rabbits divided into three groups whereas, bioavailability study in humans was an open-label, wherein 6 healthy subjects administered ondansetron gel. The potential effect of ondansetron gel was evaluated for the prevention of different phases of emesis motivated by exposure to antineoplastic drugs (cisplatin) by determination of body weight loss, water and food intake applying kaolin-pica model in rats using seventy-two rats divided into six groups. Results: Ondansetron gel (0.5%) showed detectable plasma concentration 22.833±2.17 ng/m1 after ¼ h and 419.55±2.17 ng/ml after 1-h post-treatment in rabbits and human respectively and concentration was maintained above-reported minimum effective concentration for more than 2.5 h for rabbits and 7 h for humans compared to 1.75 h after I. V. administration. The ondansetron gel significantly reduces all phases of cisplatin-induced emesis and a decrease in body weight, water, and food consumption was significantly attenuated. Conclusion: Based on the high efficacy of gel on emesis induced by cisplatin, and its high bioavailability, transdermal ondansetron gel could be a promising convenient system to prevent nausea and vomiting following administration of antineoplastic drugs

A New Flavonoid C-Glycoside from Celtis australis L. and Celtis occidentalis L. Leaves and Potential Antioxidant and Cytotoxic Activities

A major development over the past two decades has been the realization that free radical induced lipid peroxidation and DNA damage are associated with major health problems, e.g. cancer and ageing. Plant-derived antioxidants are increasingly found beneficial in protecting against these diseases. Celtis australis L. and Celtis occidentalis L. are two plants that have a variety of uses in folk medicine but have not been evaluated before for their antioxidant and cytotoxic properties. Therefore, the extracts of both plants’ leaves were investigated for these activities, as well as isolation of the bioactive compounds responsible for the activities. Molecular structures of the compounds were elucidated by UV, HRESIMS, 1D (1H and 13C) and 2D (1H-13C HSQC and 1H-13C HMBC) NMR analyses. The ethanolic and aqueous extracts, n-butanol fractions and the isolated major compound were tested for their antioxidant activity using DPPH radical scavenging assay, xanthine oxidase-induced generation of superoxide radical and lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat tissue homogenates. Cytotoxic activities were studied using standard MTT assay. A novel flavonoid C-triglycoside, 4‴-α-rhamnopyranosyl-2″-O-β-d-galactopyranosylvitexin, was isolated from both plants’ leaves, together with seven known flavonoids. The n-butanol fractions and the major compound 2″-O-β-galactopyranosylvitexin showed significant antioxidant activities, more pronounced than the tested standards BHT and dl-α-tocopherol in most tests. All extracts showed variable cytotoxic activities. This study provides strong evidence for the antioxidant and cytotoxic activities of the extracts of Celtis australis L. and Celtis occidentalis L. leaves, which were attributed to the polar n-butanol fractions and the major isolated flavonoid 2″-galactosylvitexin

Behavior of Beam to Column Cold-Formed Section Connections Subjected to Bending Moments

Cold formed sections are often used in the construction of mid-rise buildings due to their high strength weight ratios, and fast erection. In these buildings, the connections between joists and studs are mainly simple connections. However, application of these sections can be extended to moderate span frames where connections between members are subjected to bending moments. Strength and stability of such frames depends to large extent on the behaviour of the connections between their members. Over the last twenty years, several researchers undertake tests on cold formed section connections subjected to bending moments. Major of them classify the connections as semi-rigid, but some suggested that as we reach the maximum capacity of the connected sections so we can consider it rigid

DETECTION OF CHRONIC TYPHOID CARRIERS AMONG FOOD HANDLERS IN WAD MEDANI , GEZIRA STATE , SUDAN

Typhoid fever remains a disease of major public health importance in the tropics. This cross sectional prospective descriptive study was carried out between July 2005 to July 2008 in Wad Medani Town. The objective of the study was to identify chronic typhoid carriers among food handlers in Wad Medani. To achieve this objective, Vi agglutination test was used to determine suggested typhoid carriers among food handlers then stool culture was performed on those with a positive Vi agglutination test. A questionnaire was designed to collect data from suggested typhoid carriers about hygienic practices during food handling and processing .  The collected data were reviewed and coded. Data were analyzed using SPSS versions 10.0 software for tabulation and statistical analysis. The results showed that, ten percent of the examined food handlers were found positive typhoid carriers by Vi agglutination test, 48.5% of the suspected of typhoid carriers were found positive by stool culture, street vendors were more common among suspected typhoid carriers, all of whom had not received any health education about typhoid disease. The study suggested the following recommendations: Regular health authority inspection visitor food handling personnel specially street vendors to exclude typhoid carriers among them, education and training course in good hygienic practices should be provided to all food handlers specially typhoid carriers by Food Control Department, Ministry of Health, Gezira State