Oxidative status and prolidase activities in generalized anxiety disorder

SAHIN, KAZIM/0000-0002-6459-1853; Ercan, Cenk/0000-0001-9384-3653WOS: 000395258400020PubMed: 28262130Objective: Prolidase (Pro), an intracellular enzyme necessary for collagen turnover, matrix remodelling and cell growth has been shown to be related to Oxidative Stress (OS). To our knowledge, serum Pro activity in generalized anxiety disorder (GAD) has not been documented yet. in this study, we aimed to evaluate OS and its relation with Pro activity in patients diagnosed with GAD. Method: Thirty untreated GAD patients and 30 healthy controls were included in the study. Blood samples were collected from all subjects to quantify total oxidant status (TOS), total antioxidant status (TAS) and Pro activity. Oxidative stress index (OSI), the ratio of TOS to TAS, is calculated to evaluate the balance between antioxidants and oxidants. Hamilton Anxiety Rating Scale (HARS) was used to determine the anxiety levels of all subjects. Results: GAD group demonstrated statistically significantly higher TOS, OSI and Pro levels, when compared with the control group (t = 2.947, p = 0.005; t = 2.874, p = 0.006; and t = 9.396, p < 0.001 respectively). HARS scores were found to be positively correlated with TOS, OSI and Pro levels (p = 0.008, r = 0.338; p = 0.008, r = 0.339; and p < 0.001, r = 0.751 respectively). Conclusion: the degree of severity of OS is correlated with the levels of Pro. Thus, Pro might be the target enzyme, promising to be a marker for the follow-up of GAD patients. To the best of our knowledge, this study is the first to report a significant relation between Pro activity and GAD. (C) 2016 Elsevier B.V. All rights reserved

Türkiye’nin sönmeyen ateşi : Kav kibrit

Ankara : İhsan Doğramacı Bilkent Üniversitesi İktisadi, İdari ve Sosyal Bilimler Fakültesi, Tarih Bölümü, 2014.This work is a student project of the The Department of History, Faculty of Economics, Administrative and Social Sciences, İhsan Doğramacı Bilkent University.by Öztürk, İbrahim Mert

The evaluation of chromogenic medium in identification of candida species isolated from various clinical samples

Amaç: Bu çalışmada çeşitli klinik örneklerden izole edilen mayaların tür düzeyinde tanımlanmasında konvansiyonel mikolojik yöntemler ile Kromojenik besiyeri (Oxoid Brill iance™ Candida agar, İngiltere) (KB) kullanılarak yapılan tanımlamaların karşılaştırılması am açlandı. Yöntem : Otuz üç idrar, 34 transtrakeal aspirat (TTA), 7 bro n koalvaolar lavaj (BAL) örneği, 13 balgam, 12 kan, 9 yara, üç kateter ve iki vajinal akıntı olmak üzere toplam 113 klinik örnek çalışmaya alındı. İzole edilen suşların konvansiyonel mikolo jik yöntemlerle; mikroskopik ve makroskopik mo rfoloji, germ tüp testi, üre hidrolizi, ka rbonhidrat kullanımı (API 20 C AUX, Biomerieux, Fransa), üreme ısısı ve sikl o heksimid hasasiyetine göre tür tanı mlaması yapıldı. Suşlar kromojenik besiyerine (Oxoid Bri ll iance™ Candida agar, İngiltere) ekilerek renk değişimleri değerlendirildi ve her iki yöntem karşılaştırıldı. Bulgular: Çalışılan klinik örneklerde her iki yöntemle to plam 113 örneğin 103’ünde (% 91.2) aynı tür tanımlaması yapılırken,10 (%8.8) tanesinde farklı tür tanımlanmıştır. Kromejenik besiyeri C. albicans suşlarını %94.1, C. paraps ilosis %88.9 , C. glabrata %81.3 oranında doğru tanımlarken birer suş olan C. krusei ve C. tropicalis doğru, C. lusitania yanlış tanımlanmıştır. Stok kültürlerimizin 6 tan esinden birinin bakteri ile 5’inin iki çeşit Candida türü ile karışık kültür olduğu kromojenik besiyeri ile anlaşılmıştır. Sonuç : İnfeksiyon etkeni olarak sık karşılaşılan Candida suşlarının tür düzeyinde tanımlanmasında ve karışık kü ltürlerin ayırt edilme sinde kromojenik besiyerinin duyarlıl ığının yüksek olmasına rağmen nadir karşılaşılan maya türl e ri için daha fazla çalışmalara gereksinim vardır.Objective : To compare of conventional mycologic methods used to determine yeast or candida spp extracted from some clinical samples with the definitions identified using the chromogenic medium (CM, Oxoid Brilliance™ Candida agar, United Kingdom) was aimed in this study. Method : Total of 113 clinical samples (33 of urine, 34 of transtracheal aspirate (TAT), 7 of bronchoalveolar lavage (BAL), 13 of sputum, 12 of blood, 9 of wound, 3 of catheter, 2 of vaginal smear) were included. Species identification was carried out using convantional mycologic methods; microscopic and macroscopic morphology, germ tube test, urea hydrolysis, carbonhydrate use (API 20C AUX, Biom erieux, France), growth temperature and cycloheximide sensitivity. The strains were inoculated in the chromogenic medium (Oxoid Brilliance™ Candida agar, UK) and colour changes were evaluated, and two method s were comp ared. Results : With both method, 91.2% of (n=103) total 113 samples was identified as similar whilest 8.8% of them (n=10) was defined differently. 94.1% of Candida albicans spp, 88.9% of C. parapsilosis and 81.3% of C. glabrata was truly identi fied. C. krusei and C. tropicalis were correctly identified whereas C. lusitania was wrongly identified. It was understood that one of total 6 of our stock cultures was found as mixed culture with bacteria, and the rest of them was found as mixed with two types of Candida sp ecies, with using chromogenic medium. Conclusion : There is a need to further studies for rare yeast species although the sensitivity of chromogenic medium is high for using the determination of common candida sp ecies as infectious agents.

The negative prognostic impact of bone metastasis with a tumor mass

OBJECTIVE:Typically, bone metastasis causes osteolytic and osteoblastic lesions resulting from the interactions of tumor cells with osteoclasts and osteoblasts. In addition to these interactions, tumor tissues may grow inside bones and cause mass lesions. In the present study, we aimed to demonstrate the negative impact of a tumor mass in a large cohort of patients with bone metastatic cancer.METHODS:Data from 335 patients with bone metastases were retrospectively reviewed. For the analysis, all patients were divided into three subgroups with respect to the type of bone metastasis: osteolytic, osteoblastic, or mixed. The patients were subsequently categorized as having bone metastasis with or without a tumor mass, and statistically significant differences in median survival and 2-year overall survival were observed between these patients (the median survival and 2-year overall survival were respectively 3 months and 16% in patients with a tumor mass and 11 months and 26% in patients without a tumor mass; p<0.001).RESULTS:According to multivariate analysis, the presence of bone metastasis with a tumor mass was found to be an independent prognostic factor (p=0.011, hazard ratio: 1.62, 95% confidence interval: 1.11–1.76). Bone metastasis with a tumor mass was more strongly associated with osteolytic lesions, other primary diseases (except for primary breast and prostate cancers), and spinal cord compression.CONCLUSION:Bone metastasis with a tumor mass is a strong and independent negative prognostic factor for survival in cancer patients

The Prognostic Value Of Uhrf-1 And P53 In Gastric Cancer

Background/Aims: This study aimed to examine whether UHRF-1 and p53 overexpression is a prognostic marker for gastric cancer. Patients and Methods: Sixty-four patients with gastric cancer (study group) and 23 patients with gastritis (control group) were evaluated. Immunohistochemistry was used to examine expression of UHRF-1 and p53 in gastric cancers and a control group diagnosed with gastritis. Results: The median age was 63 years (18-83 years) in the study group. UHRF-1 was positive in 15 (23%) patients with gastric cancer and five (21.7%) patients with gastritis (P = 0.559). UHRF1 expression level in gastric cancer is more powerful than in gastritis (P = 0.046). Thirty-seven (61%) patients with gastric cancer and only one patient with gastritis were p53 positive (P < 0.001). After a median follow-up of 12 months (1–110), the 2-year overall survival rates were 55% and 30% in negative and positive p53, respectively (P = 0.084). Also, the 2-year overall survival rates were 45% and 53% in negative and positive UHRF-1, respectively (P = 0.132). Conclusion: According to this study, UHRF-1and p53 were not prognostic factors for gastric cancer, whereas they may have a diagnostic value for differantiating between gastric cancer and gastritis.PubMedWoSScopu