PHYTOCHEMICAL STUDY OF BIOACTIVE CONSTITUENTS FROM SATUREJA MONTANA L. GROWING IN EGYPT AND THEIR ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES

 Objective: This work aimed to investigate the lipid constituents and flavonoidal compounds of Satureja montana, in addition to evaluation of different extracts and/or isolated compounds as antimicrobials and antioxidants.Methods: The volatile and lipid constituents were extracted with n-hexane by partition from hydroalcoholic extract of S. montana L. aerial parts, after then were fractionated to unsaponifiable matters and fatty acid methyl esters which were identified by gas–liquid chromatography and/or gas chromatography–mass spectrometry. The phenolic constituents were isolated from the ethyl acetate fraction of the aqueous methanolic extract of the aerial parts of the plant. The antimicrobial activity of different extracts and the isolated compounds was evaluated against Gram-positive, Gram-negative bacteria, yeast, and fungus using a modified Kirby-Bauer disc diffusion method.Results: The identified compounds are luteolin-7-rhamnoside-4'-O-β-glucopyranoside (1), quercetin-3-O-α-L-rhamnopyranoside (2), quercetin- 7-O-glucopyranoside (3), luteolin-7-O-glucopyranoside (4), 5-hydroxy-6,7,8,4'-tetramethoxy flavone (5), gallic acid (6), 2,3-hexahydroxydiphenoyl 1-galloyl glucopyranoside (7), and quercetin (8). The structure of all isolated compounds was established using different chromatographic and spectroscopic measurements (PC, thin-layer chromatography, ultraviolet [UV], 1D, 2D-nuclear magnetic resonance, and MS). Compound-2 showed the highest antibacterial activity against all the tested microorganisms. Hydroalcoholic extract exhibited high antioxidant activity (87.7%). On the other hand, hexane fraction showed a low antioxidant activity (46.4%), in addition to the compound-8 showed the highest antioxidant activity (96.27%) in 2,2-diphenyl-1-picrylhydrazyl assay.Conclusion: It can be concluded that the hydroalcoholic extract of S. montana showed significant antimicrobial and antioxidant activity

INVESTIGATION OF SOME CHEMICAL CONSTITUENTS AND ANTIOXIDANT ACTIVITY OF ASPARAGUS SPERNGERI

Objective: Asparagus sprengeri (A S) is an ornamental plant cultivated in EGYPT. This study was designed to investigate some lipid and flavanoid constituents, in addition to the evaluation of antioxidant activity of different extracts of the plant. Methods: Two phospholipid were identified by (HPTLC). The fatty acid methyl ester and unsaponifiable matter were analyzed by GC. Four flavonoids were isolated by CC and purified by PPc. Identification of isolated flavanoidal compounds was carried by spectroscopic analysis Viz, TLC,PC, UV, EL-MS and H-1– NMR. The different extracts were tested for their free radical scavenging activity using DPPH and β-Carotene- linoleic acid bleaching assay. Results: Four flavonoids, were isolated, apigenin, dihydroquercitin, naringenin, apigenin- 7-o- glucoside Nine fatty acids, Myrestic and linoliec acids were the major components. The unsaponifiable matter was found to be a mixture of hydrocarbons from (C13– C28) and cholesterol two classes of phospholipid were identified namely L-α-Phosphatidyl-DL-glycerol and L-α- Phosphatidylethanolamine. The alcoholic extract of the plant has moderate antioxidant activity with EC50 0.114 and 0.110 mg/ml for the DppH and β-carotene methods respectively Conclusion: Asparagus sprengeri can be used as the natural antioxidant. Flavonoids are suggested to be a group of key antioxidants in Asparagus

Study of Some Chemical Constituents and Antioxidant Activity of Beaumontia grandiflora Wall. grown in Egypt

of hydrocarbons (n-C -n-C ) in addition to â-sitosterol, stigmasterol, cholesterol, campasterol and á-amyrine.The flavonoidal constituents were isolated from the ethyl acetate fraction of the alcoholic extract. Four flavonoids were identified as; quercetin i, quercetin-3-O-galactoside ii, diosmetin-7-Orhamnoside iii and kaempferol-3-O-neohesperidoside iv. The crude extracts (total alcoholic, ethyl acetate, and butanol extracts) and the isolated compounds were screened for their antioxidant activity using DPPH radical Scavenging method. Ethyl acetate extract and compound 1 showed high antioxidant activity compared to Trolox (standard antioxidant compound) 91, 89% respectively

Vicia faba peel extracts bearing fatty acids moieties as a cost-effective and green corrosion inhibitor for mild steel in marine water: computational and electrochemical studies

Abstract The goal of this research is to determine what chemicals are present in two different extracts (hexane and acetone) of Vicia faba (family Fabaceae, VF) peels and evaluate their effectiveness as a corrosion inhibitor on mild steel in a saline media containing 3.5% sodium chloride. Gas chromatography-mass spectrometry (GC/MS) was used to determine the composition of various extracts. It was determined that fourteen different chemicals were present in the hexane extract, the most prominent of which were octacosane, tetrasodium tetracontane, palmitic acid, and ethyl palmitate. Heptacosane, lauric acid, myristic acid, ethyl palmitate, and methyl stearate were some of the 13 chemicals found in the acetone extract. Using open circuit potential, potentiodynamic polarisation, and electrochemical impedance spectroscopic techniques, we can approximate the inhibitory effects of (VF) extracts on mild steel. The most effective inhibitory concentrations were found to be 200 ppm for both the hexane and acetone extracts (97.84% for the hexane extract and 88.67% for the acetone extract). Evaluation experiments were conducted at 298 K, with a 3.5% (wt/v) NaCl content and a flow velocity of about 250 rpm. Langmuir adsorption isotherm shows that the two extracts function as a mixed-type inhibitor in nature. Docking models were used to investigate the putative mechanism of corrosion inhibition, and GC/MS was used to identify the major and secondary components of the two extracts. Surface roughness values were calculated after analyzing the morphology of the metal's surface with and without (VF) using a scanning electron microscope (SEM). The results showed that throughout the surface of the mild steel, a thick adsorbate layer was formed. Quantum chemical calculations conducted on the two extracts as part of the theoretical research of quantum chemical calculation demonstrated a connection between the experimental analysis results and the theoretical study of the major chemical components

Removal of Lead(II) from Synthetic Wastewater by Lavandula pubescens Decne Biosorbent: Insight into Composition–Adsorption Relationship

In this work, the widely-abundant, cheap, wild plant Lavandula pubescens Decne was evaluated as an adsorbent for removing Pb(II) ions from wastewater. The chemical composition of the plant was partially isolated and characterized by the corresponding techniques, including gas chromatography–mass spectrometry, gas liquid chromatography, and FTIR spectroscopy. The adsorption capacity of the dried plant material for Pb(II) ions increased with increasing contact time, initial ion concentration, and temperature, while it decreased with increasing adsorbent dosage. The optimum condition for Pb(II) adsorption was determined as 550 mg/L initial metal concentration, pH ≤ 7, and 90 min of contact. The best fit for Pb(II) adsorption isotherms was the linear form of the Freundlich model; however, the maximum capacity indicated by Langmuir was 91.32 mg/g. The experimental data fit better the pseudo-second-order kinetic model (R2 = 0.969), suggesting chemisorption process. Thermodynamic data revealed an endothermic, nonspontaneous, and adsorption process favored at higher concentrations