The immediate upstream region of the 5 '-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana

The nucleotide sequence around the translational initiation site is an important cis-acting element for post-transcriptional regulation. However, it has not been fully understood how the sequence context at the 5'-untranslated region (5'-UTR) affects the translational efficiency of individual mRNAs. In this study, we provide evidence that the 5'-UTRs of Arabidopsis genes showing a great difference in the nucleotide sequence vary greatly in translational efficiency with more than a 200-fold difference. Of the four types of nucleotides, the A residue was the most favourable nucleotide from positions -1 to -21 of the 5'-UTRs in Arabidopsis genes. In particular, the A residue in the 5'-UTR from positions -1 to -5 was required for a high-level translational efficiency. In contrast, the T residue in the 5'-UTR from positions -1 to -5 was the least favourable nucleotide in translational efficiency. Furthermore, the effect of the sequence context in the -1 to -21 region of the 5'-UTR was conserved in different plant species. Based on these observations, we propose that the sequence context immediately upstream of the AUG initiation codon plays a crucial role in determining the translational efficiency of plant genes.ope

식물에서의 단백질 대량 생산 시스템의 구축과 활용

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Arabidopsis dynamin-like protein 2 is involved in the biogenesis of chloroplast in Arabidopsis

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Targeting of Proteins to the chloroplast outer envelope membranes

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Multiple pathways for controlling ABA homeostasis.

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AtBG2, a beta-glucosidase homolog localized in the vacuole increases cellular ABA via hydrosis of ABA-GE.

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Molecular players involved in vacuolar trafficking of proteins in plant cells?

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Adaptors for clathrin-mediated trafficking in plant cells.

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Can the protein targeting mechanisms serve as molecularfossil to reveal the evolution of two endosymbiotic organelles, chloroplasts and mitochondria?

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Trafficking of proteins to two different vacuoles, the protein storage vacuole and the central vacuole.

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