Apparatus and method for determining Alzheimer's disease score using multi-channel fluorescence lifetime signals

다채널 형광 시상수 신호를 이용한 알츠하이머 지수 판단 장치 및 방법이 개시된다. 일 실시예에 따른 알츠하이머 지수 판단 장치는 피검체에 광을 조사하고 상기 피검체로부터 방출되는 형광신호를 다채널로 검출하여, 채널별 형광신호 세기 영상, 채널별 형광신호의 시상수 영상, 및 채널간 형광신호 세기의 비율 영상을 생성하는 형광시상수 이미징 현미경; 상기 채널별 형광신호 세기 영상, 상기 채널별 형광신호의 시상수 영상, 및 상기 채널간 형광신호 세기의 비율 영상 각각의 대표값을 산출하는 대표값 산출부; 및 각 영상의 대표값을 기반으로 상기 피검체의 알츠하이머 지수를 판단하는 알츠하이머 지수 판단부; 를 포함할 수 있다

비축수차 및 짧은 시야심도가 개선된 내시경용 고분해능 광간섭단층촬영용 이미징 프루브 개발

Endoscopic micro optical coherence tomography (μOCT) is a biological imaging modality which provides high resolution endoscopic three dimensional imaging in vivo. Despite its usefulness, there are following several limitations in practical applications. First of all, cylindrical sheath protecting the probe inherently causes astigmatism. Second, high numerical aperture makes shorter depth of field (DOF). These are critical because astigmatism degrades image quality and short DOF restrains imaging range in axial direction. To address these aforementioned limitations, we developed a new imaging probe incorporating a zone plate pattern and multimode fiber (MMF). One dimensional zone plate pattern was inserted right after GRIN lens to resolve the astigmatism. And self-imaging wavefront division optical system using multimode fiber made coaxially focused multimode beam to extend depth of field. We designed the 1-D zone plate pattern using optical simulation software (OpticStudio, Zemax) and fabricated by direct laser writing technique. We connected optical elements using glass processor (GPX3300, Vytran) and epoxy. Furthermore, we evaluated the performance of our probe by getting point spread function using beam profiler. The proposed probe showed promising performance for improved image quality and imaging range of micro optical coherence tomography

Establishing In Vitro 3D Sarcopenia Model Using Skeletal Muscle-on-a-Chip

Sarcopenia, the progressive loss of muscle mass that occurs with aging, leads to impaired movement, increased risk of injury, and reduced quality of life. The pathogenic roles of advanced glycation end-products (AGEs) in skeletal muscle have been reported, but practical experiments to assess the impact of AGEs on skeletal muscle from a mechanical standpoint are lacking. To address this issue, we have developed a skeletal muscle-on-a-chip system that generates 3D skeletal muscle tissue and measures muscular morphology and contractile function. Using this system, we investigate the impact of AGEs on skeletal muscle for the first time, demonstrating their damaging effects on contractile function, myotube degeneration, and cell death in skeletal muscle tissue. Our results are confirmed through various methods, including contraction force measurement, biochemical assays, Next Generation Sequencing (NGS), and Fluorescence-lifetime imaging microscopy (FLIM). This study provides a 3D in vitro sarcopenia model using an organ-on-a-chip system, which enables the investigation of the impact of AGEs on skeletal muscle. Our approach offers a new perspective on sarcopenia and provides a platform for investigating other substances as well