Research of Design and Valve Dynamics in Mechanical Electro Hydraulic Valve Train

학위논문 (박사)-- 서울대학교 대학원 : 기계항공공학부, 2011.2. 민경덕.Docto

Protective effect of original endotoxin protein and possible immunological mechanism

Pseudomonas aeruginosa has been known as one of the major causative microorganisms of the opportun: istic infection. However, a variety of chemotherapeutic :m easures have been found to be unsatisfactory because of its intrinsic resistance to most of currently used chemotherapeutic agents and of its ability to acquire antimicrobial resistance. Therefore, immunological measures have been tried as an alternative approach to preγent pseudomonas infection. In this study, original endotoxin protein(OEP) , which was purified from autolytic culture lysates of P. aeruginosa N•10 , was studied 、，v i t h respect to its preventive effect in pseudomonas infection and its possible mechanisms of action. The preventive effect was evaluated by comparing LDso of P. aeruginosa in burned mice with or 、iv i t h o u t OEP immunization (5μg/mouse， twice) The protective mechanism of OEP 、 as studied in the aspect of the humoral immune response and phagocytic and bactericidal activities of peripheral polymorphonuclear leukocytes in the immunized mice. The results were as follows. 1. LD50 of P. aeruginosa was significantly (0、er the 95% confidence interval) increased in the OEP immune ICR mice, against challenge with 13 serotYr:es of P. aeruginosa isolated from clinical specimen of Seoul National University Hospital 2. After OEP immunization, rising of agglutinin titer to 10 kinds of P. aeruginosa serotype were olserved. Ten serotypes was as follow; A(Pa-13), DCPa 48) , E(Pa-16) , GCPa•55) , H(Pa-79) , I(Pa•49) , ](Pa-84), K(Pa-80) , LCPa-83) , and M(Pa-85) But agglutinins to 3 kinds of P. aeruginosa, serotype B(Pa-4) , C(Pa•29) and F serotype (Pa9) were not ohserved. 3. Only 5. 696 of P. aerugiηosa were survived after reacting with the OEP immune rabbit serum with complement for 24 hrs , at 4'C. Decreasing viable P. aerugiηosa after reacting with the OEP immune rabbit serum was partially inhibited by absorbing the OEP immune serum with Pa-13. 4. When rabbit peripheral hlood was reacted with Pa-13 (5 X 107 microorganism/ml) for 1 hour at 37°C, phagocytic act ivity was found in 80.096 of polymorphonuclear leukocytes from OEP immune rabbit and 28.9% from normal rabbit 5. Number of viable P. aeruginosa was decreased to 6% after reacting with the OEP immune rabbit blood for 1 hour at 37°C, in contrast it was decreased to 77% in normal rabbit blood

충남공공디자인클리닉센터 하반기 워크숍 (이충훈, 최명식, 김상열)

1-1. 1차 운영위원 워크숍 □ 목 적 ○ 충남의 경쟁력 향상을 위한 공공디자인 추진 방안 ○ 지자체 공공디자인 자문 및 컨설팅 방향 모색 □ 개 요 일 시 : 2009년 3월 27일(금) 15:00~17:30 장 소 : 충남발전연구원 1층 대회의실 주제발표 : 최명식 교수(경희대학교) – “지자체 공공디자인 추진정책” 김상열 선임연구원(한국디자인진흥원) – “공공디자인 컨설팅 방향” 이충훈 센터장(공공디자인클리닉센터) – “충남공공디자인 기본계획” -이후 생략충남 공공디자인클리닉센터 하반기 워크숍 ￭01_2009 충남공공디자인클리닉센터 운영성과 및 내년도 계획 ￭02_충청남도 공공디자인 기본계획 (이충훈 충남발전연구원) 계획의 배경 및 목적 기본구상 가이드라인 상세계획 실행계획 ￭03_(공주시) "최고와 일등:"을 추구하는 디자인 명품도시 지역소개 디자인 정책이념 및 전략 디자인 경영활동 디자인 경영성과 디자인 공주의 미래 ￭04_(논산시)같은 공간에서 만난 다섯가지 이야기 '논산오거리 거리공간 조성사업' 사업개요 추진현황 문제점 및 대책 논산오거리 거리공간조성사업 ￭05_(아산시) 2009아산시 공공디자인 추진사례 일반현황 주요 추진 사업 소