8 research outputs found
Protein domain prediction for protein tertiary structure prediction
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ผ๋ฌธ (์์ฌ)-- ์์ธ๋ํ๊ต ๋ํ์ : ํ๋๊ณผ์ ์๋ฌผ์ ๋ณดํ์ ๊ณต, 2015. 8. ์์ฐจ์ฅ.Protein structure prediction results provide important biological information such as structural, functional analysis of proteins and protein design. Protein structure prediction methods can be divided into two categories. First, ab initio modeling methods predict structure based on physical principles. Second, Template-based modeling methods predict structures by selecting template structures from the database of known experimental structures. This can lead to more accurate predictions.
Protein domain information further improves the predictive power of template-based modeling. This is because protein domain information improves the fold recognition step, one of the main parts of template-based modeling, which in turn determines the quality of final predicted protein structure. However, they do not account for the fact that some multi-domain proteins have related proteins with experimental structures of multiple domains resolved together in the structure database. The goal of GalaxyDom is to assign modeling units such that the maximal information can be extracted from the structure database for structure prediction of each unit, allowing prediction of domain-domain interactions as well as individual domain structures when reliable templates are available in the database.
Our method uses HHsearch as the fold recognition tool. We use CASP domain definition in order to evaluate the true protein domain boundary. The CASP domain definition is determined by the CASP assessor and the true domain boundary is ยฑ 10 residues. Also, the protein domain predictions of GalaxyDom find better templates than other domain prediction toolsโ results. The better the method is at predicting reliable modeling unit regions, the better the ability of template-based modeling to find better template proteins.1. Introduction 1
1.1. Structure and Function: Why do we need protein structure? 1
1.2. Protein structure prediction overcomes the limitation of experimental methods 3
1.3. Protein domain prediction improves ability of fold recognition and Modeling unit detection 7
1.4. Outline of this thesis 10
2. Methods 11
2.1. Overall procedure of GalaxyDom-PDB and GalaxyDom-PDB/SCOP 11
2.2. Selection of PDB and SCOP templates 14
2.3. Calculation of chunk score 15
2.4. Determination of modeling units 19
2.5. Comparison with other domain prediction methods 24
2.6. Test set 25
3. Results and Discussion 26
3.1. Detection of modeling units 26
3.2. Effect of domain prediction on template selection 30
4. Conclusions 38
Appendix 39
Bibliography 41
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