14 research outputs found

    ํญํ’์˜์–ธ๋• ์—ฐ๊ตฌ:Catherine์˜ ๋น„๊ทน์„ ์ค‘์‹ฌ์œผ๋กœ

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    ๋Œ€ํ•™์ƒ์˜ ์ง„๋กœ๊ฒฐ์ •์ˆ˜์ค€ ๋ฐ ์ง„๋กœํƒ์ƒ‰ํ–‰๋™๊ณผ ์„ฑ๊ฒฉ 5์š”์ธ์˜ ๊ด€๊ณ„

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    ํ•™์œ„๋…ผ๋ฌธ(์„์‚ฌ)--์„œ์šธ๋Œ€ํ•™๊ต ๋Œ€ํ•™์› :๊ต์œกํ•™๊ณผ ๊ต์œก์ƒ๋‹ด์ „๊ณต,1999.Maste

    Jane Eyre์—ฐ๊ตฌ : ์—ฌ์„ฑ์ƒ์„ ์ค‘์‹ฌ์œผ๋กœ

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    1970๋…„๋Œ€ ํ›„๋ฐ˜ ์ดํ›„ Elaine Showalter, Susan Gubar, Sandra Gilbert, Judith Newton ๋“ฑ์˜ ์—ฌ์„ฑ๋ก  ๋น„ํ‰๊ฐ€๋“ค์€ Brontรซ ์ž‘ํ’ˆ์— ๋Œ€ํ•ด ์ƒˆ๋กœ์šด ํ•ด์„์˜ ์žฅ์„ ์—ด์–ด ์ฃผ์—ˆ๋‹ค. ์ด๋“ค์€ Jane์˜ ๊ฒฝํ—˜์„ ๊ฐ•๋ ฌํ•˜๋‚˜ ์ฃผ๊ด€์ ์ธ ๊ฐœ์ธ์  ๊ฒฝํ—˜์— ๊ทธ์น˜๋Š” ๊ฒƒ์œผ๋กœ ๋ณธ David Cecil๊ณผ๋„, Jane์˜ ๊ฒฝํ—˜ ์†์—์„œ ํ† ์ง€ ์  ํŠธ๋ฆฌ์™€ ์‚ฐ์—…๊ณ„๊ธ‰ ๊ฐ„์˜ ๊ฐˆ๋“ฑ๊ณผ ํƒ€ํ˜‘์˜ ๋ฒ”์ฃผ์  ๊ตฌ์กฐ๋ฅผ ์ฐพ๋Š” Eagleton๊ณผ๋„ ๋‹ค๋ฅธ ์ž…์žฅ์—์„œ Jane Eyre์— ์ ‘๊ทผํ•œ๋‹ค. ์ด๋“ค์€ R. Williams๊ฐ€ ๋‹ค์†Œ ๋ง‰์—ฐํ•˜๊ฒŒ ์–ต์•• ๊ตฌ์กฐ๋ผ๊ณ  ํ–ˆ๋˜ ๊ฒƒ ๋Œ€์‹  ๊ฐ€๋ถ€์žฅ์ œ ์‚ฌํšŒ์˜ ์–ต์•• ์•ž์—์„œ ์ž์‹ ์˜ ์ •์ฒด์„ฑ์„ ํ™•์ธํ•ด๊ฐ€๋Š” ์—ฌ์„ฑ์œผ๋กœ์„œ์˜ Jane์˜ ๊ฒฝํ—˜์— ์ฃผ๋ชฉํ•œ๋‹ค. Elaine Showalter๋Š” ์—ฌ์„ฑ์„ ์ •์‹ ์ ์ธ ์กด์žฌ์ธ ๊ฐ€์ •์˜ ์ฒœ์‚ฌ๋‚˜ ์„ฑ์˜ ํ™”์‹ ์ธ ์œก์ฒด์˜ ์•…๋งˆ๋กœ ์–‘๋ถ„ํ•ด์„œ ๋ณด๋Š” ์‚ฌํšŒ์˜ ์–ต์•• ์•ž์—์„œ Jane์ด ํš๋“ํ•œ ์œก์ฒด์™€ ์ •์‹ ์˜ ํ†ตํ•ฉ์„ ์ฃผ๋กœ ๊ณ ์ฐฐํ•˜๊ณ  ์žˆ์œผ๋ฉฐ, Gilbert์™€ Gubar ์—ญ ์‹œ Madwoman in the Attic์—์„œ ์‚ฌํšŒ๊ฐ€ ๊ฐ•์š”ํ•˜๋Š” ์ด์ƒ์ ์ธ ์—ฌ์„ฑ์ƒ์€ ๊ฐ€์ •์˜ ์ฒœ์‚ฌ์ธ ์ ์— ์ฃผ๋ชฉํ•œ๋‹ค. ๊ทธ๋Ÿฌ๋‚˜ Gilbert์™€ Gubar๋Š” Showalter์ฒ˜๋Ÿผ ์„ฑ์˜ ๋ฌธ์ œ์— ์ง‘์ค‘ํ•˜์ง€ ์•Š๊ณ  ๊ฐ€์ •์˜ ์ฒœ์‚ฌ๋ฅผ ์ˆ˜๋™์ ์ด๋ฉฐ ์ž์•„๋ฅผ ์ƒ์‹คํ•œ ์กด์žฌ๋กœ ํŒŒ์•…ํ•˜๊ณ , ๊ฐ€๋ถ€์žฅ์ œ๊ฐ€ ๊ฐ•์š”ํ•˜๋Š” ์ˆœ์‘์ ์ธ ์—ญํ• ์„ ๊ฑฐ๋ถ€ํ•˜๋Š” ์—ฌ์„ฑ์€ ๊ดด๋ฌผ๋กœ ํˆฌ์‚ฌ๋จ์„ ์ง€์ ํ•œ๋‹ค. Gilbert์™€ Gubar๋Š” Jane Eyre์˜ ํ•ต์‹ฌ์ ์ธ ๋ฌธ์ œ๊ฐ€ ์„ฑ์˜ ๋ฌธ์ œ๊ฐ€ ์•„๋‹ˆ๊ณ  ์‚ฌํšŒ์˜ ๊ธฐ์ค€์— ๋Œ€ํ•œ Jane์˜ ๋ฐ˜ํ•ญ๊ณผ ๋ถ„๋…ธ์ด๋ฉฐ, ์ด๊ฒƒ์€ ์ง์ ‘ ๋‚˜ํƒ€๋‚˜๋Š” ๊ฒƒ์ด ์•„๋‹ˆ๋ผ, Jane์˜ double์•ˆ Bertha์˜ ํ–‰๋™์— ํˆฌ์‚ฌ๋œ๋‹ค๊ณ  ๋ณธ๋‹ค

    Ego Identity Status and Confirmation Bias in Career Information Search

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    The purpose of this study is to examine the differences in confirmation bias in career information search among ego identity statuses. The present study focuses specifically on the differences between individuals with a foreclosed ego identity status, those who have simply adopted the opinions and attitudes of their significant others such as parents without proper exploration, and individuals with an achievement ego identity status, those who have explored several alternatives and then established their identity. Participants were selected from undergraduate students at teachers colleges, located in Seoul, and Kyeong-gi province. A total of 737 students participated in this study; 548 (74.4%) were women and 189(25.6%) were men. K-EOMEIS-2 was utilized to measure ego identity status. A selective exposure paradigm was used to measure confirmation bias in career information search. In order to construct the selective exposure paradigm, occupational information on teachers was given. This occupational information on teachers consisted of 16 articles. Each article was summarized by a title and a main thesis (two or three sentences). The procedures were followed as the general procedure of experiments based on the cognitive dissonance theory. In order to focus on the effects of ego identity status, confidence and grade variables (which were proved to be related to confirmation bias in the preliminary analysis) were controlled. Confirmation bias was measured in terms of the selection and the evaluation of information. The findings from the current study have been summarized below. First, there were significant differences in confirmation bias in terms of the selection of information among ego identity statuses. The foreclosed students showed the most confirmation bias. In other words, the extent to which the foreclosed students selected the supporting information exclusively was larger than the students in the other statuses. The students in moratorium status showed the second highest level of confirmation bias, followed by students with achievement status. The students with a diffusion status showed the lowest level in confirmation bias in the selection of information. Second, there were also significant differences in confirmation bias in the evaluation of information among ego identity statuses. There were significant differences in the evaluation of supporting information; however, there were no significant differences in the evaluation of threatening information. In the evaluation of supporting information, students with foreclosure and moratorium statuses wanted to read the supporting information more and evaluated the supporting information as more important compared to the students in diffusion and achievement statuses. In conclusion, the foreclosed students showed the highest level of confirmation bias in the selection of information. They also showed higher level of confirmation bias in the evaluation of supporting information; however, there was no significant difference in the evaluation of threatening information among ego identity statuses. Implications for career guidance and counseling, and directions for future research were discussed.๋ณธ ์—ฐ๊ตฌ์˜ ๋ชฉ์ ์€ ์ง„๋กœ ์ •๋ณด ํƒ์ƒ‰์— ์žˆ์–ด์„œ ์ž์•„์ •์ฒด์„ฑ ์ง€์œ„๋“ค ๊ฐ„์˜ ํ™•์ฆํŽธํŒŒ์˜ ์ฐจ์ด๋ฅผ ์‚ดํŽด๋ณด๊ณ ์ž ํ•˜๋Š” ๊ฒƒ์ด๋‹ค. ํŠนํžˆ, ๋ณธ ์—ฐ๊ตฌ๋Š” ์ ์ ˆํ•œ ํƒ์ƒ‰์—†์ด ๋ถ€๋ชจ์™€ ๊ฐ™์€ ์ค‘์š”ํ•œ ํƒ€์ธ์˜ ์˜๊ฒฌ๊ณผ ํƒœ๋„๋ฅผ ๊ทธ๋Œ€๋กœ ๋ฐ›์•„๋“ค์—ฌ ์ž์‹ ์˜ ์ •์ฒด์„ฑ์„ ํ˜•์„ฑํ•œ ์ž์•„์ •์ฒด์„ฑ ์กฐ๊ธฐ์™„๋ฃŒ ์ง€์œ„์™€ ์—ฌ๋Ÿฌ ๋Œ€์•ˆ๋“ค์„ ํƒ์ƒ‰ํ•˜๊ณ  ๋‚˜์„œ ์ž์‹ ์˜ ์ •์ฒด์„ฑ์„ ํ™•๋ฆฝํ•œ ์„ฑ์ทจ ์ง€์œ„ ์‚ฌ์ด์˜ ์ฐจ์ด์ ์— ์ดˆ์ ์„ ๋‘์—ˆ๋‹ค. ์—ฐ๊ตฌ ์ฐธ๊ฐ€์ž๋“ค์€ ์„œ์šธ๊ณผ ๊ฒฝ๊ธฐ ์ง€์—ญ์— ์œ„์น˜ํ•œ ๊ต๋Œ€์ƒ๋“ค์„ ๋ชจ์ง‘ํ•˜์˜€๋‹ค. ์ด 737๋ช…์˜ ํ•™์ƒ์ด ์ฐธ์—ฌํ•˜์˜€๊ณ  ์—ฌํ•™์ƒ์ด 548(74.4%)๋ช…์ด์—ˆ๊ณ  ๋‚จํ•™์ƒ์€ 189(25.6%)๋ช… ์ด์—ˆ๋‹ค. ํ•œ๊ตญ์–ดํŒ ์ž์•„์ •์ฒด์„ฑ์ง€์œ„๊ฒ€์‚ฌ(EOM-EISII)๋ฅผ ์‚ฌ์šฉํ•˜์—ฌ ์ž์•„์ •์ฒด์„ฑ์ง€์œ„๋ฅผ ์ธก์ •ํ•˜์˜€๋‹ค. ์ง„๋กœ ์ •๋ณด ํƒ์ƒ‰์—์„œ์˜ ํ™•์ฆํŽธํŒŒ๋ฅผ ์ธก์ •ํ•˜๊ธฐ ์œ„ํ•ด์„œ ์„ ํƒ์  ๋…ธ์ถœ ํŒจ๋Ÿฌ๋‹ค์ž„์„ ํ™œ์šฉํ•˜์˜€๋‹ค. ์„ ํƒ์  ๋…ธ์ถœ ํŒจ๋Ÿฌ๋‹ค์ž„์„ ๊ตฌ์„ฑํ•˜๊ธฐ ์œ„ํ•ด, ๊ต์‚ฌ์— ๊ด€ํ•œ ์ง์—… ์ •๋ณด๋ฅผ ์ž‘์„ฑํ•˜์˜€๋‹ค. ๊ต์‚ฌ์— ๊ด€ํ•œ ์ •๋ณด๋Š” ์ œ๋ชฉ๊ณผ 2-3์ค„์˜ ๊ฐ„๋žตํ•œ ์š”์•ฝ๋ฌธ์œผ๋กœ ์ด๋ฃจ์–ด์ง„ 16๊ฐœ ๊ธฐ์‚ฌ๋กœ ๊ตฌ์„ฑ๋˜์—ˆ๋‹ค. ์—ฐ๊ตฌ์ ˆ์ฐจ๋Š” ์ธ์ง€๋ถ€์กฐํ™” ์ด๋ก ์— ๊ทผ๊ฑฐํ•œ ์ผ๋ฐ˜์ ์ธ ์‹คํ—˜์ ˆ์ฐจ๋ฅผ ๋”ฐ๋ž๋‹ค. ์ž์•„์ •์ฒด์„ฑ์ง€์œ„์˜ ํšจ๊ณผ์— ์ดˆ์ ์„ ๋‘๊ธฐ ์œ„ํ•ด, ์˜ˆ๋น„ ๋ถ„์„์—์„œ ํ™•์ฆํŽธํŒŒ์™€ ๊ด€๋ จ์ด ์žˆ๋Š” ๊ฒƒ์œผ๋กœ ๋ฐํ˜€์ง„ ํ™•์‹ ๋„์™€ ํ•™๋…„ ๋ณ€์ธ์€ ํ†ต์ œํ•˜์˜€๋‹ค. ํ™•์ฆํŽธํŒŒ๋Š” ์ •๋ณด์˜ ์„ ํƒ๊ณผ ํ‰๊ฐ€๋ผ๋Š” ์ธก๋ฉด์—์„œ ์ธก์ •๋˜์—ˆ๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ ์–ป์€ ๊ฒฐ๊ณผ๋“ค์„ ์š”์•ฝํ•˜๋ฉด ๋‹ค์Œ๊ณผ ๊ฐ™๋‹ค. ์ฒซ์งธ, ์ •๋ณด์˜ ์„ ํƒ์ด๋ผ๋Š” ์ธก๋ฉด์—์„œ ์ž์•„์ •์ฒด์„ฑ ์ง€์œ„๊ฐ„์— ์œ ์˜๋ฏธํ•œ ํ™•์ฆํŽธํŒŒ์˜ ์ฐจ์ด๊ฐ€ ์žˆ์—ˆ๋‹ค. ์กฐ๊ธฐ์™„๋ฃŒ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์ด ๊ฐ€์žฅ ๋งŽ์€ ํ™•์ฆํŽธํŒŒ๋ฅผ ๋ณด์˜€๋‹ค. ์ฆ‰, ์กฐ๊ธฐ์™„๋ฃŒ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์ด ๋ฐฐํƒ€์ ์œผ๋กœ ์ง€์ง€ ์ •๋ณด๋ฅผ ์„ ํƒํ•œ ์ •๋„๊ฐ€ ๋‹ค๋ฅธ ์ง€์œ„์˜ ํ•™์ƒ๋“ค๋ณด๋‹ค ๋” ์ปธ๋‹ค. ์œ ์˜ˆ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์€ ๋‘๋ฒˆ์งธ๋กœ ๋†’์€ ์ˆ˜์ค€์˜ ํ™•์ฆํŽธํŒŒ๋ฅผ ๋ณด์˜€๊ณ , ๋‹ค์Œ์€ ์„ฑ์ทจ ์ง€์œ„์˜€๋‹ค. ํ˜ผ๋ฏธ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์€ ์ •๋ณด ์„ ํƒ์—์„œ ๊ฐ€์žฅ ๋‚ฎ์€ ์ˆ˜์ค€์˜ ํ™•์ฆํŽธํŒŒ๋ฅผ ๋ณด์˜€๋‹ค. ๋‘˜์งธ, ์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ๋„ ์ž์•„์ •์ฒด์„ฑ ์ง€์œ„๊ฐ„ ์œ ์˜๋ฏธํ•œ ์ฐจ์ด๊ฐ€ ์žˆ์—ˆ๋‹ค. ์ง€์ง€์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ๋Š” ์œ ์˜๋ฏธํ•œ ์ฐจ์ด๊ฐ€ ์žˆ์—ˆ์œผ๋‚˜, ์œ„ํ˜‘์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ๋Š” ์œ ์˜๋ฏธํ•œ ์ฐจ์ด๊ฐ€ ์—†์—ˆ๋‹ค. ์ง€์ง€ ์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ, ์กฐ๊ธฐ์™„๋ฃŒ์™€ ์œ ์˜ˆ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์€ ํ˜ผ๋ฏธ์™€ ์„ฑ์ทจ ์ง€์œ„์˜ ํ•™์ƒ๋“ค ๋ณด๋‹ค ์ง€์ง€์ •๋ณด๋ฅผ ๋” ์ฝ๊ณ  ์‹ถ์–ดํ–ˆ๊ณ  ์ง€์ง€์ •๋ณด๋ฅผ ๋” ์ค‘์š”ํ•˜๊ฒŒ ํ‰๊ฐ€ํ–ˆ๋‹ค. ๊ฒฐ๋ก ์ ์œผ๋กœ, ์กฐ๊ธฐ์™„๋ฃŒ ์ง€์œ„์˜ ํ•™์ƒ๋“ค์€ ์ •๋ณด์˜ ์„ ํƒ์—์„œ ๊ฐ€์žฅ ๋†’์€ ์ˆ˜์ค€์˜ ํ™•์ฆ ํŽธํŒŒ๋ฅผ ๋ณด์˜€๋‹ค. ๊ทธ๋“ค์€ ๋˜ํ•œ ์ง€์ง€ ์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ๋„ ๋†’์€ ์ˆ˜์ค€์˜ ํ™•์ฆํŽธํŒŒ๋ฅผ ๋ณด์˜€์ง€๋งŒ, ์œ„ํ˜‘์ •๋ณด์˜ ํ‰๊ฐ€์—์„œ๋Š” ์ž์•„์ •์ฒด์„ฑ ์ง€์œ„๊ฐ„์— ์œ ์˜๋ฏธํ•œ ์ฐจ์ด๊ฐ€ ์—†์—ˆ๋‹ค. ์ด๋Ÿฌํ•œ ๊ฒฐ๊ณผ๋ฅผ ๋ฐ”ํƒ•์œผ๋กœ, ์ง„๋กœ ์ง€๋„์™€ ์ƒ๋‹ด์— ๋Œ€ํ•œ ํ•จ์˜์™€ ํ–ฅํ›„ ์—ฐ๊ตฌ ๋ฐฉํ–ฅ์„ ๋…ผ์˜ํ•˜์˜€๋‹ค.Docto

    ์ข… ํŠน์ด์  ๋ฏธํ† ์ฝ˜๋“œ๋ฆฌ์•„ ์„œ์—ด์„ ํ™œ์šฉํ•œ ์‹ ์†์œ ์ „์ž๋“ฑ์˜จ์ฆํญ๊ธฐ๋ฒ•์˜ ์œก์ข… ๊ฐ๋ณ„ ์ ์šฉ

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    ์ตœ๊ทผ ์ผ๋ จ์˜ ์‹์œก๋ถˆ๋ฒ• ํ˜ผ์ž… ์‚ฌ๊ฑด ๋“ฑ์€ ์ด์™€ ๊ฐ™์€ ์‹์œก ๋ถˆ๋ฒ• ํ˜ผ์ž…์„ ์ •ํ™•ํžˆ ํŒ๋ณ„ํ•ด ๋‚ผ ์ˆ˜ ์žˆ๋Š” ๊ณผํ•™์ ์ธ ๊ธฐ๋ฒ•์— ๋Œ€ํ•œ ์†Œ๋น„์ž์˜ ์š”๊ตฌ๋ฅผ ์ฆ๊ฐ€์‹œ์ผฐ๋‹ค. ์ด๋Ÿฌํ•œ ์†Œ๋น„์ž์˜ ์š”๊ตฌ๋ฅผ ์ถฉ์กฑ์‹œํ‚ค๊ณ ์ž ์‹์œก ๊ฐ๋ณ„์„ ์œ„ํ•œ ์œ ์ „์ž์ฆํญ๊ธฐ๋ฒ•์€ ๋‹ค์–‘ํ•˜๊ฒŒ ๊ฐœ๋ฐœ ๋ฐ ํ™œ์šฉ๋˜์–ด ์™”๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ๋Š” ์ข… ํŠน์ด์  ๋ฏธํ† ์ฝ˜๋“œ๋ฆฌ์•„ ์„œ์—ด์„ ํ™œ์šฉํ•œ ์‹ ์†์œ ์ „์ž๋“ฑ์˜จ์ฆํญ๊ธฐ๋ฒ•์„ ๊ฐœ๋ฐœํ•˜์—ฌ ์†Œ, ๋ผ์ง€, ๋ง, ์—ผ์†Œ, ์–‘, ๋‹ญ, ์˜ค๋ฆฌ, ์น ๋ฉด์กฐ ์ด 8์ข…์˜ ์‹์œก์„ ํŠน์ด์ ์œผ๋กœ ๊ฒ€์ถœ ๋ฐ ๊ฐ๋ณ„ํ•˜๊ณ ์ž ํ•˜์˜€๋‹ค. ์ข… ํŠน์ด์  ๋ฏธํ† ์ฝ˜๋“œ๋ฆฌ์•„ ์„œ์—ด์„ ํ™œ์šฉํ•˜์—ฌ LAMP ํ”„๋ผ์ด๋จธ๋ฅผ ์ œ์ž‘ํ•˜์—ฌ ๊ฐ๊ธฐ ๋‹ค๋ฅธ annealing temperature๊ฐ€ ์„ค์ •๋˜๋„๋ก ํ•˜์˜€๊ณ , LAMP ์‹œ์•ฝ ๋‚ด intercalating dye๋ฅผ ์ฒจ๊ฐ€ํ•˜์—ฌ ๋ฐ˜์‘์‚ฐ๋ฌผ์˜ ์ฆํญ ๋ฐ ๋ถ„์„์„ ์‹ค์‹œ๊ฐ„ ๊ด€์ฐฐํ•  ์ˆ˜ ์žˆ๋„๋ก ํ•˜์˜€๋‹ค. ๊ฐ๊ฐ์˜ LAMP๊ธฐ๋ฒ•๋ณ„ ์ตœ์  ๋ฐ˜์‘ ์˜จ๋„ ๋ฐ ์‹œ์•ฝ ์กฐ๊ฑด์„ ์„ค์ •ํ•˜์˜€๊ณ  8์ข…์˜ ์‹์œก์„ 3๊ทธ๋ฃน์œผ๋กœ ๋ถ„๋ฅ˜ํ•œ ํ›„ ์‹œํ—˜ํ•œ ๊ฒฐ๊ณผ, ๊ต์ฐจ ๋ฐ˜์‘ ์—†์ด ๋ชจ๋‘ 100%๋กœ ๊ด€์ฐฐ๋˜์—ˆ์œผ๋ฉฐ ๊ฐ ์ข…๋งˆ๋‹ค ๊ณ ์œ ํ•œ ๊ฐ’์„ ๋‚˜ํƒ€๋‚ด์–ด ์ด๋ฅผ ํ† ๋Œ€๋กœ ๊ทธ๋ฃน ๋‚ด์—์„œ ํŠน์ • ์‹์œก ์ข…์„ ์„ฑ๊ณต์ ์œผ๋กœ ๋ถ„๋ฅ˜ํ•  ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๊ฐœ๋ฐœ๋œ LAMP๊ธฐ๋ฒ•์„ ์›๋ฃŒ์œก, ๊ฐ€์—ด์œก, ํ˜ผํ•ฉ์œก, ํ˜ผํ•ฉ๊ฐ€์—ด์œก ๋“ฑ ์ด 4๊ฐœ์˜ ์‹œ๋ฃŒ๊ตฐ์— ์ ์šฉํ•˜์—ฌ ๊ฒ€์ถœํ•œ๊ณ„๋ฅผ ๊ด€์ฐฐํ•˜์˜€๋‹ค. ์›๋ฃŒ์œก ๋ฐ ๊ฐ€์—ด์œก์—์„œ์˜ LAMP ๊ฒ€์ถœํ•œ๊ณ„๋Š” 10 pg/ฮผl to 100 fg/ฮผl์˜ ๋ฒ”์œ„์—์„œ ๊ด€์ฐฐ๋˜์—ˆ๊ณ , ํ˜ผํ•ฉ์œก ๋ฐ ํ˜ผํ•ฉ๊ฐ€์—ด์œก์—์„œ์˜ LAMP ๊ฒ€์ถœํ•œ๊ณ„๋Š” 0.01% to 0.0001%์˜๋ฒ”์œ„์—์„œ๊ด€์ฐฐ๋˜์—ˆ๋‹ค. ๋˜ํ•œ, ์‹ค์‹œ๊ฐ„์œผ๋กœ ๊ด€์ฐฐ๋œ ์ฆํญ์‚ฐ๋ฌผ์„ ํ† ๋Œ€๋กœ 30๋ถ„ ์ด๋‚ด์— ์ •๋Ÿ‰ํ™” ๊ณก์„ ์„ ๋„์ถœํ•  ์ˆ˜ ์žˆ์—ˆ๊ธฐ์— ๋งค์šฐ ์‹ ์†ํ•˜๊ณ  ์‹ค์‹œ๊ฐ„ ์ •๋Ÿ‰์ด ๊ฐ€๋Šฅํ•œ ๊ธฐ๋ฒ•์ž„์„ ์•Œ ์ˆ˜ ์žˆ์—ˆ๋‹ค. ๋ณธ ์—ฐ๊ตฌ์—์„œ ๊ฐœ๋ฐœํ•œ LAMP๊ธฐ๋ฒ•์€ ์‹ ์†์„ฑ, ๋ฏผ๊ฐ๋„, ์ •ํ™•๋„๊ฐ€ ๋†’์€ ์œ ์ „์ž ๋ถ„์„ ๊ธฐ๋ฒ•์œผ๋กœ์„œ ์‹์œก ์ข…์˜ ๊ฐ๋ณ„์„ ๋ณด๋‹ค ์‰ฝ๊ฒŒ ์ˆ˜ํ–‰ํ•  ์ˆ˜ ์žˆ์„ ๊ฒƒ์œผ๋กœ ๊ธฐ๋Œ€๋˜๋ฉฐ, ํ–ฅํ›„ ํ‘œ์ค€๋ฒ•๊ณผ์˜ ๋ณ‘ํ–‰ ํ™œ์šฉ ๊ฐ€์น˜ ๋˜ํ•œ ๋†’์„ ๊ฒƒ์œผ๋กœ ๊ธฐ๋Œ€๋œ๋‹ค.Meat fraud and adulteration has led to consumer demands for accurate methods of meat source. LAMP assays targeting species-specific mitochondrial DNA were designed to identify and discriminate eight meat species based on annealing curve analysis: cattle, pig, horse, goat, sheep, chicken, duck, and turkey. The LAMP primer sets were designed on the basis of a consensus of several mitochondrial sequences for each species. No cross-reaction was observed and all eight species were successfully discriminated by their unique annealing temperatures. The limits of detection (LoDs) of the LAMP assays in raw and cooked meat were determined at 10 pg/ยตl to 100 fg/ยตl levels, and those in raw and cooked meat admixtures were determined at 0.01% to 0.0001% of target meat levels within 30 min. The newly designed LAMP assays are a simple, rapid, accurate, and sensitive method for discrimination of eight meat species and could be used for meat species identification.ABSTRACT-------------------------------i CONTENTS------------------------------iii LIST OF ABBREVIATION----------------------iv INTRODUCTION---------------------------1 MATERIALS AND METHODS-------------------5 RESULTS AND DISSCUSION--------------------10 REFERENCES----------------------------28 ABSTRACT IN KOREAN (๊ตญ๋ฌธ์ดˆ๋ก)---------------38Maste
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