Absorbable Guided Bone Regeneration Membrane Fabricated from Dehydrothermal Treated Porcine Collagen

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuide?) compared to of the non-crosslinked porcine type I, III collagen (BioGide?) and the glutaldehyde cross-linked bovine type I collagen (BioMend?) in surgically created bone defects in rat mandible. Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuide?, BioMend?, or BioGide? (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A 3×4 mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done. 원고접수일 2010년 9월 20일, 게재확정일 2011년 1월 4일책임저자 이종호(110-749) 서울시 종로구 연건동 28번지, 서울대학교 치의학대학원 구강악안면외과학교실Tel: 02-2072-2630, Fax: 02-766-4948, E-mail: [email protected] RECEIVED September 20, 2010, ACCEPTED January 4, 2011Correspondence to Jong-Ho Lee Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University 28, Yeongun-dong, Chongro-gu, Seoul 110-749, Korea Tel: 82-2-2072-2630, Fax: 82-2-766-4948, E-mail: [email protected] CC This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-Gide? membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed. Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGide? and BioMend?, thus, could be clinically useful.N