A composition for prevention and treatment of fish viral hemorrhagic septicemia containing extracts of Ecklonia cava

본 발명은 감태 추출물 또는 그로부터 유래된 성분을 포함하는 바이러스성 출혈성 패혈증 (viral hemorrhagic septicemia, VHS)에 대한 예방 또는 치료용 약학 조성물, 상기 약학조성물을 개체에 투여하는 단계를 포함하는 바이러스성 출혈성 패혈증의 예방 또는 치료 방법, 감태 추출물로부터 유래된 성분을 포함하는 바이러스성 출혈성 패혈증 바이러스에 대한 항바이러스용 조성물, 감태 추출물 또는 그로부터 유래된 성분을 포함하는 사료첨가용 조성물 및 상기 사료첨가용 조성물을 포함하는 사료를 어류에 섭식시키는 단계를 포함하는 어류의 사육방법에 관한 것이다.ope

Implication of CD69(+)CD103(+) tissue-resident-like CD8(+) T cells as a potential immunotherapeutic target for cholangiocarcinoma

Background The heterogeneous immune landscapes of intrahepatic cholangiocarcinoma (ICC) remain largely unknown. Here we aimed to investigate the implications of tissue-resident memory (TRM)-related features of tumour-infiltrating CD8(+) T cells (CD8(+) TILs) from ICC patients. Methods From ICC patients, we obtained blood samples and ICC surgical specimens (n = 33). We performed multicolour flow cytometry, multiplexed immunohistochemistry and RNA sequencing. Results When compared to peripheral CD8(+) T cells, the CD8(+) TILs included significantly higher proportions of the CD69(+)CD103(-) and CD69(+)CD103(+) TRM-like subsets (P < .001 for both). Relative to CD69(-) and CD69(+)CD103(-) cells, the CD69(+)CD103(+) CD8(+) TILs harboured higher levels of T-cell markers representing tumour specificity (ie CD39), proliferation (ie Ki-67) and T-cell activation (ie HLA-DR and CD38) (all P < .001). Moreover, compared to the stroma, the tumour margin and core density each had a significantly higher density of CD103(+) CD8(+) TILs (P < .001 for both). ICCs with high proportions of CD69(+)CD103(+) cells displayed higher levels of parameters associated with response to immune checkpoint inhibitors (ICIs)-including number of CD8(+) TIL infiltrates (P = .019), PD-L1 expression in the tumour (P = .046) and expression of the T cell-inflamed gene signature (P < .001). ICCs with lower proportions of CD69(+)CD103(+) CD8(+) TILs exhibited significant enrichment of genes related to the Wnt/beta-catenin (P < .001) and TGF-beta pathways (P = .002). Conclusion CD69(+)CD103(+) TRM-like CD8(+) TILs represent prominent tumour-specific immune responses and hold promise as a potential therapeutic target in ICC patients. Differential TRM-related features of ICCs may help develop future immunotherapeutic strategies such as maximizing TRM responses or inhibiting pathways contributing to immune evasion