자성 다층 박막에서의 공명 연 X-선 광자기 효과에 관한 연구

Thesis(doctors) --서울대학교 대학원 :재료공학부,2010.2.Docto

Genetic studies on mitochondrial factors that regulate anti-bacterial immunity in Caenorhabditis elegans

DoctorMitochondria are important subcellular organelles that exert multiple functions in eurkaryotic cells. For the last decade, many studies have shown that mitochondria play key roles in cellular immunity against pathogens. However, immune functions of mitochondria at the organismal level remain incompletely understood. Here, we identified 16 evolutionarily conserved mitochondrial components that affected the immunity of C. elegans against pathogenic bacteria, Pseudomonas aeruginosa (PA14), by performing a large scale genetic screen. Subsequent analyses revealed that the mitochondrial components modulated anti-bacterial immunity through distinct immune pathways. Among the immune-regulatory mitochondrial factors, mitochondrial chaperone HSP-60 was necessary and sufficient for increasing resistance against PA14. We demonstrated that HSP-60 increased the activity of p38 MAP kinase (PMK-1), which boosted the anti-PA14 immunity. In addition, we found that a fraction of HSP-60 localized to the cytosol and cytosolic HSP-60 was sufficient for increasing PA14 resistance. Cytosol-localized HSP-60 physically interacted with and stabilized MAP kinase kinase (SEK-1), which in turn up-regulated p38 MAP kinase signaling and immunity. Altogether, our data showing the HSP-60-dependent transmission of immune-regulatory signals from mitochondria to the cytosol provides fascinating insights into the role of crosstalk between organelles in host defense mechanisms. Furthermore, our study proposes an intriguing possibility that organisms exploit a component of mitochondria, which may have originated from ancient symbiotic bacteria, to protect host animals via monitoring pathogenic bacteria

SREBP and MDT-15 reduce toxic effects of glucose on lifespan by promoting fat synthesis in C. elegans

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A genome-wide RNAi screen for the identification of genes involved in the life-shortening effect by glucose

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Identification of glucose-regulatory genes using a genome-wide RNAi screen.

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Functional characterization of genes crucial for the effects of glucose on lifespan identified through a genome-wide RNAi screen

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The role of cpsf-2/cleavage and polyadenylation specificity factor 2 in C. elegans lifespan regulation

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Method for displaying multifunctional cellulolytic enzyme in bacterial cell surface and use thereof

본 발명은 다기능 셀룰라아제(multifunctional cellulase)의 세포표면 발현방법 및 이의 용도에 관한 것으로, 상세하게는 소의 루멘(rumen) 부유물에서 추출한 메타게놈 라이브러리로부터 로봇 기반의 초고속 탐색 시스템을 이용하여 스크리닝한, 엑소-셀룰라아제, 엔도-셀룰라아제 및 β-글루코시다아제의 기능을 모두 가지고 있는 다기능 셀룰라아제를 세포 외막 단백질 C(Omp C)를 부착 모티프로 하여 대장균의 세포표면에 발현하는 방법에 관한 것이다.국

The role of Krebs cycle metabolites in innate immunity in C. elegans

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Identification and Characterization of Glucose-regulatory Genes through a Genome-wide RNAi Screen in C. elegans

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