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배출시설관리

TRUETRU

알긴산올리고당유도체를이용한가축질병예방및치료제

발명요약

Methods for risky species management at ports in Korea

According to the GloBallast Programme, relative risk-species threat can be acquired from the equation such as (NIS + [suspected harmfulsｘ3] + [Known harmfulsｘ10]) / Total sum All Source Ports for calculation of relative overall risk coefficients.The three categorized species lists are represented as the number of species recorded from the field surveys and retrospective data in our study. The present risk assessment is primarily based on the port-to-port comparativeness, and furthermore, on the bio-region to bio-region comparison on global scale. Thus, we have tried to collect the species lists that are assigned to the three levels of threat from the four major ports (Incheon, Gwangyang, Pusan and Ulsan) corresponding to the three bio-regions (NWP4c, NWP3a and NWP4a, orderly). Besides, to acquire species references to distinguish native species from introduced species in the near future, port environmental and biological investigations have been conducted seasonally at the major ports since last year.Taking consideration of potential risk identification qualitatively or quantitatively, abundance and composition of species are simultaneously analyzed from the samples collected seasonally at the ports. Even though incomplete lists of species and constraints in the identifications reliability exist in the constructing the species database, consultation for confirmation of the species lists has been carried out through taxonomic specialists in Korea. From the port environment, non-indigenous species, suspected and known harmful organisms have been considered and categorized based on the information from previously published and already settled organisms by unpublished reports. To fill the gaps of species lists from the periodical and literature investigations, species lists observed in the ship’s ballast water tanks have been also investigated from various types of ships entering the four ports. And, adaptation experiments of foreign species via ballast wat2

적조원인종인 Prorocentrum minimum 의 온도에 따른 생화학적 조성의 변화

22othe

Spatio-temporal distribution in copepods of the genus Acartia at ports in Korea

Present study described basically occurring pattern of copepods of genus Acartia on the seasonal basis and tried to find ballast water-mediated introduced species from natural zooplankton community at major ports. Mesozooplankton samples were taken at four major ports such as Incheon, Gwangyang, Pusan and Ulsan since 2007. The number and position of stations sampled were identical, and samples were collected by using same gear (60 cm in diameter and 200㎛ mesh standard type net. Mesozooplankton samplings were conducted using the R.V.󰡒Jangmok󰡓at ports of Pusan and Ulsan and were collected using the ships of opportunity at ports of Incheon and Gwangyang. The collected zooplankton were identified and enumerated under a stereomicroscope (ZEISS Model Stemi-2000C) in a laboratory. Acartia hongi occurred across all seasons, while A. hudsonica showed up in spring and fall at Incheon port. A. omorii and A. pacifica occurred only in summer. A. omorii showed up at four ports during the study periods. Distribution of A. hudsonica was confined to Incheon port, even though its habitat area is not limited to the coastal waters of the Yellow Sea. A. hudsonica occurs at three seas, but was not widely distributed compared to A. omorii. A. hongi consistently occurred at Incheon port and the adjacent waters which was known as one of their original habitat. Interestingly, the copepod showed up at ports of Gwangyang, Pusan and Ulsan beyond their biogeographic range on May and August 2007, and May 2008. A. erythraea showed only at Gwangyang port in summer, and at Pusan port in summer and fall only inside the Pusan port. A. danae was found outside the ports of Ulsan and Pusan in Fall. Exceptionally, A. negligens was observed at station 9 outside the port of Pusan. In the present study, only 6 species were found at ports of Incheon, Gwangyang, Ulsan and Pusan, which did not include entire coastal waters of Korea during the study periods.2

Methods for risky species management at ports in Korea

2

저수온기, 동해연안의 식물플랑크톤 군집 조사

대양에서 미소플랑크톤(20 ㎛)과 미소플랑크톤(<20 ㎛)으로 나누어 현존량을 조사하기 시작하였다. 특히 저수온기에 미소 플랑크톤이 식물플랑크톤 현존량의 50% 이상을 차지하였다는 연구가 보고되었다. 따라서 본 연구는 저수온기에 동해에서 식물플랑크톤의 군집조성을 조사하기 위해, 속초에서부터 울산 내에 10개의 정점을 선정하여, 2012년 3월 중 2회 현장조사를 수행하였다. 식물플랑크톤의 크기에 따라 미세플랑크톤과 미소플랑크톤으로 구분하였으며, 미소플랑크톤은 형태에 따라 각각 나누어 분류하였다. 조사 기간 중, 수온은 8.24∼13.05 ℃, 염분은 26.25∼35.35 PSU로 조사 지점에 따라 큰 차이를 보였다. 식물플랑크톤의 현존량은 1.94×105∼4.36×106 cells L-1로 조사되었으며, 미소플랑크톤의 현존량은 전체 식물플랑크톤 현존량의 최고 80% 이상을 차지하는 지역도 관찰되었다. 그리고 20 여 종의 미세플랑크톤과 F(filament), O(oval), OF(oval & flagella), R(rod), S(spherical) type의 미소플랑크톤이 관찰되었다. 전체 식물플랑크톤 출현종 중 미세플랑크톤은 규조류의 비중이 가장 높게 나왔으며, 미소플랑크톤은 S type이 일부지역에서 60% 이상을 차지하여 가장 높은 비중을 차지하였다. 본 연구를 통하여, 동해에서 저수온기에 미소플랑크톤의 현존량이 큰 비중을 차지하고, 또한 다양한 type으로 구성되어 있음을 확인할 수 있었다. 향후 미소플랑크톤에 대한 분류와 생태학적 특성을 이해하는 것이 동해 생태사하기 시작하였다. 특히 저수온기에 미소 플랑크톤이 식물플랑크톤 현존량의 50% 이상을 차지하였다는 연구가 보고되었다. 따라서 본 연구는 저수온기에 동해에서 식물플랑크톤의 군집조성을 조사하기 위해, 속초에서부터 울산 내에 10개의 정점을 선정하여, 2012년 3월 중 2회 현장조사를 수행하였다. 식물플랑크톤의 크기에 따라 미세플랑크톤과 미소플랑크톤으로 구분하였으며, 미소플랑크톤은 형태에 따라 각각 나누어 분류하였다. 조사 기간 중, 수온은 8.24∼13.05 ℃, 염분은 26.25∼35.35 PSU로 조사 지점에 따라 큰 차이를 보였다. 식물플랑크톤의 현존량은 1.94×105∼4.36×106 ce2

Preliminary results of planktonic copepod, Acartia omorii as biomarker for environmental toxicity

Zooplankton is an important component of the food webs of marine and brackish ecosystems. Copepod zooplankton is very abundant and plays as a mediator between phytoplankton and small fish in the food webs. Ecotoxicological risk to zooplankton is evaluated by the biomarker approach, can be used as an early warning signal of risk to the health of marine ecosystems. We conducted two experiments to estimate the toxicity of benzo[a]pyrene and pore water on a copepod species Acartia omorii (i) effects of benzo[a]pyrene on DNA single strand breakage and (ii) toxicity of pore water on mortality. DNA damage was observed in homogenates of whole organisms. DNA strand breakage in individual cells increased with addition of benzo[a]pyrene. It was similar to H2O2 using as positive control. Pore water was extracted from bottom sediments of Gwangyang Bay, which is located in the southern part of Korea. In all sampling sites, the mortality of the copepod increased with addition of pore water, and the response of copepod to pore water was significantly different among the sampling sites. It might be related to the concentrations of organic pollutants in sediments. This study suggests that Acartia omorii can be used as a biomarker for monitoring of environmental pollution1