(The)Effect of regular exercise on cardiopulmonary function, inflammation marker, and heart rate variability in patients with chronic heart failure

학위논문(박사)--서울대학교 대학원 :체육교육과,2005.Docto

RDBMS 기반의 토픽맵 무결성 검사 기법

학위논문(석사)--서울대학교 대학원 :전기·컴퓨터공학부,2004.Maste

메틸자스몬산에 의한 수확후 딸기 과실의 착색 및 안토시아닌 생합성 유도

학위논문 (석사)-- 서울대학교 대학원 : 식물생산과학부 원예생명공학 전공, 2016. 2. 이은진.Strawberry (Fragaria × ananassa) is an important fruit worldwide. The fruit has high nutritional value such as anthocyanins and ascorbic acid. However, strawberry fruit is delicate and highly perishable to mechanical injury, desiccation, and physiological disorders after harvest. Methyl jasmonate (MeJA), a naturally occurring plant endogenous hormone, plays an important role in plant development, fruit ripening, and defense mechanism. Furthermore, postharvest application of MeJA has been reported to effectively enhance antioxidant capacity in berry fruits including strawberry and suppress postharvest diseases caused by necrotrophic pathogens. In this study, the effects of postharvest MeJA treatment on anthocyanin biosynthesis and postharvest decay in Seolhyang strawberry fruit were investigated. Peduncles of strawberry fruits were immersed in 100 μM MeJA solution and stored at 10 ± 0.5oC and 90 ± 5% relative humidity for 3 weeks. Fruits firmness and respiration rate were not significantly affected by MeJA. Red coloration by MeJA was significantly increased to a greater degree compared to that in control. Total anthocyanins contents were higher in MeJA treatment (27.5 ± mg·100g-1 fresh weight) than in control (13.4 ± mg·100g-1 fresh weight) at 2 weeks in storage. Changes in phenylalanin ammonia-lyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and flavonoid 3-O-glucosyltransferase (UFGT) mRNA levels involved in anthocyanin biosynthesis were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR). PAL and CHS expression levels were higher than CHI, F3H, DFR, ANS, and UFGT expression levels in control fruits at 3 and 6 days in storage. CHI, F3H, DFR, ANS, and UFGT expression levels in MeJA treatment were higher than those in control fruit at 6 and 8 days in storage. On the other hand, the expression level of genes involved in anthocyanin biosynthesis were higher in control fruits than in MeJA treated fruits after 10 days in storage. These results suggest that postharvest MeJA treatment on Seolhyang strawberry fruits increased the red coloration by up-regulating mRNA expression of these genes during early storage. The MeJA treatment significantly reduced fruit decay. At the end of storage, decay rate in MeJA treatment was only 21%, whereas 67% of fruit decay was shown in control fruit. Exogenous MeJA treatment also increased endogenous MeJA contents in the strawberry fruits. These results indicated that MeJA can effectively improve red coloration by increasing anthocyanin biosynthesis and reduce decay on Seolhyang strawberry fruits. Thus it will help to elucidate the ripening mechanism of non-climacteric strawberry fruit, and it will be beneficial in distributing the high quality strawberry through developing the decay inhibition technique by MeJA.INTRODUCTION 1 LITERATURE REVIEW 4 1. Seolhyang strawberry 4 2. Role of plant hormone to induce fruit ripening and inhibit decay 7 3. Jasmonate 10 MATERIALS AND METHODS 14 1. Plant material 14 2. MeJA treatment 14 3. Fruit quality assessments 16 4. Anthocyanin analysis 17 5. Gene expression analysis 19 6. Statistical analysis 24 RESULTS 25 1. Changes of fruit quality and physiological parameters of strawberry fruit by MeJA treatment 25 2. Quantitative analysis of anthocyanin and biosynthesis gene expression in Seolhyang strawberry fruit by MeJA treatment 28 3. Quantitative analysis of biosynthesis gene expression in Seolhyang strawberry fruit by MeJA treatment 35 DISCUSSION 37 REFERENCES 41 ABSTRACT IN KOREAN 48Maste

견관절의 반복적인 등속성 운동에 따른 근피로도와 회전력에 관한 상관분석

학위논문(석사)--서울대학교 대학원 :체육교육과,1999.Maste